Bambusicola loculata D.Q. Dai & K.D. Hyde, 2015

Dai, Dong-Qin, Bahkali, Ali H., Li, Wen-Jing, Bhat, D. Jayarama, Zhao, Rui-Lin & Hyde, Kevin D., 2015, Bambusicola loculata sp. nov. (Bambusicolaceae) from bamboo, Phytotaxa 213 (2), pp. 122-130 : 127

Bambusicola loculata D.Q. Dai & K.D. Hyde , sp. nov. Figure 2

Index Fungorum number: IF551064

Facesoffungi number: FoF 00587

Etymology: In reference to the loculate ascostromata.

Diagnosis: this new species differs from other species in the genus as it has multi-loculate ascostromata and is phylogenetically resolved from species where the asexual morph is only known.

Holotype: MFLU 15–0056 .

Saprobic on dead bamboo culms. Sexual morph: Ascostromata 150–200 μm high, 350–600 μm diam., solitary to clustered, or two in groups, becoming erumpent, but remaining immersed under host tissue at maturity, with a central ostiolar opening when mature, with 1–3 locules; individual locules 150–180 μm high, 200–300 μm diam., immersed in stromata, subglobose, with centrally located ostiole lined with periphyses. Peridium comprising host and fungal tissues, 5–10 μm thick at upper side, composed of brown and thick-walled cells of textura angularis, with basal part composed of thinner, hyaline, smaller cells, with side wall composed of 30–80 μm wide cells of textura prismatica. Hamathecium of dense, long and up to 1 μm wide pseudoparaphyses, branching and anastomosing above the asci. Asci 80–105 × 8–13 μm (= 90.1 × 11.8 μm, n = 20), 8-spored, bitunicate, cylindric-clavate, with a short furcate pedicel, with a shallow ocular chamber. Ascospores 22–26.5 × 5–6 μm (= 24.5 × 5.2 μm, n = 20), 2–3-seriate, narrowly fusiform, 1-septate, constricted at the septum, slightly curved, smooth-walled, narrowly rounded at both ends, hyaline, surrounded by an inconspicuous mucilaginous sheath. Asexual morph: Undetermined.

Culture on PDA: —Ascospores germinating on PDA within 24 h and germ tubes produced at both ends. Colonies slow growing, 40 mm diam. in 45 days at 25–32ºC, circular, with uneven margin, light-colored at the periphery, floccose and hyaline to light greenish brown from above; pure yellow at the centre from reverse.

Material examined: — THAILAND. Chiang Rai: Doi Mae Salong, temple side, on dead culm of bamboo, 15 August 2013, Dong-Qin Dai DDQ00266 ( MFLU 15–0056 , holotype), ibid. ( KUN! HKAS 86441 , isotype) ; ex-type living culture at MFLUCC 13–0856 and CBS 139961 .

Notes: — Bambusicola loculata differs from the uniloculate B. massarinia and B. bambusae in having multi-loculate ascostromata. In addition, the asci of the new species are longer than those of two latters (90.1 × 11.8 μm vs. 70.1 × 12 μm and 56.6 × 6.6 μm). Bambusicola irregulispora and B. splendida are only known from their asexual morphs. Moreover the new taxon is distinguished from the other species in the phylogenetic analyses ( Fig. 1 ). The new species can be morphologically and phylogenetically distinguish from Palmiascoma gregariascomum (99%/1.00 MLBS / BYPP support) ( Fig. 1 ) which has brown ascospores ( Liu et al. 2015).

Figures

FIGURE 1. Maximum likelihood (RAxML) tree based on combined dataset of LSU, SSU, RPB2 and TEF sequence. Bootstrap support (BS) values for RAxML above 50% and Bayesian posterior probabilitie grate than 0.95 are shown near nodes. Hyphen (“--”) indicates a value lower than 50 % (BS) or 0.90 (BYPP). The original strain numbers are noted after the species names. Ex-type or ex-epitype strains are with asterisk, and the type species are indicated in blue. Newly species obtained in this study is in bold and highlight.The tree is rooted with Halojulella avicenniae (BCC 18422).

FIGURE 2. Bambusicola loculata (MFLU 15-0056, holotype). A, B. Ascomata developing on bamboo culm. C, D. Section of ascoma. E. Peridium of ascoma. H–G, P. Asci and pseudoparaphyses. J. Germinating ascospore. K–O, Q–S. Hyaline ascospores. T, U. Culture on PDA. Scale bars:A, B = 500 μm, C = 200 μm, D, E = 50 μm, F–J = 10 μm, K–S = 5 μm, T = 25 mm, U=15 mm.