Strain

Lee, So-Yeon, Han, Jeong Eun & Bae, Pil Soo Kim and Jin-Woo, 2020, A report of 18 unrecorded prokaryotic species isolated from the feces of an Oriental stork (Ciconia boyciana), and from the intestinal tracts of a cobitid fish (Kichulchoia multifasciata) and a Korean splendid dace (Coreoleuciscus splendidus), Journal of Species Research 9 (4), pp. 325-338 : 326

Strain isolation and characterization

The feces of C. boyciana were collected at the Seoul Grand Park Zoo (N 37°25 ʹ 22.3 ʺ E 127°1 ʹ 17.8 ʺ). Intestinal tracts of K. multifasciata and C. splendidus were collected from streams in Gimcheon-si, Gyeongsangbuk-do (N 36°05 ʹ 54.9 ʺ E 128°07 ʹ 32.1 ʺ), and Gapyeong-gun, Gyeonggi-do (N 37°51 ʹ 55.3 ʺ E 127°21 ʹ 00.9 ʺ), Republic of Korea, respectively. All feces and intestinal samples were homogenized in sterile phosphate buffered saline and serially diluted. Samples were spread onto three different culture media (marine agar, tryptic soy agar, and Reasoner’s 2A agar; BD Difco) and incubated at 10°C and 30°C. We repeated several sub-cultivations using the streak-plating method to obtain pure isolates.