Crassiparies quadrisporus Matsumura, K. Hiray. & Kaz. Tanaka

Lu, Yu-Hang, Zhang, Sheng-Nan, Du, Hong-Zhi, Cheewangkoon, Ratchadawan & Liu, Jian-Kui, 2024, Additions to Crassiparies and Neobrevicollum (Neohendersoniaceae, Pleosporales) associated with woody hosts in Southwest China, Phytotaxa 636 (2), pp. 126-138 : 134-135

Crassiparies quadrisporus Matsumura, K. Hiray. & Kaz. Tanaka , in Fungal Diversity 78: 63 (2016), Fig. 4 MycoBank: MB 815295

Saprobic on the branch of Jasminum nudiflorum and Camellia sinensis . Sexual morph: Ascomata 210–250 µm high, 255–270 µm diam (x = 235 × 260 µm, n = 10), scattered, sometimes in groups of 2–4, immersed, globose to subglobose, with a central ostiole. Peridium 15–30 µm wide, outer layers dark brown to black, inner layers thin-walled, composed of hyaline cells of textura angularis. Hamathecium 2.0–2.5 µm wide, numerous, dense, hyaline, septate, filamentous pseudoparaphyses. Asci 65–145 × 13–18 µm (x = 93 × 15 µm, n = 30), 4-spored, bitunicate, fissitunicate, cylindric-clavate or clavate, short pedicellate, apically rounded. Ascospores 24–30 × 8–12 µm (x = 26×10 µm, n = 50), hyaline, broadly fusiform, overlapping biseriate, ends rounded, 1-septate, with a septum mostly submedian, minutely echinulate, guttulate, without mucilaginous sheath. Asexual morph: Undetermined.

Culture characteristics: —Ascospores germinated within 24 hours on PDA, Colonies reaching about 25 mm diam after three weeks, irregular, with undulate edge, light brown to dark brown at the surface with white margin and brown from the centre of the colony in reverse with white margin. Mycelium 2.4–3.6 μm broad, hyaline to pale brown, septate, branched. Chlamydospores apical or intercalary, produced after 150 days of growth on PDA at 25 °C, 4.9–16.0 × 4.0–10.5 μm, ellipsoidal, thick-walled, pale brown when young, brown when mature.

Material examined: — CHINA, Sichuan province, Chengdu city, Chengdu Botanical Garden, 30°45ʹ52ʺN, 104°7ʹ35ʺE, 535m elevation, 21 September 2022, on branches of Jasminum nudiflorum ( Oleaceae ) in a terrestrial habitat, Y.H. Lu & H.Z. Du, C05 (HUEST 23.0134), living culture UESTCC 23.0134; ibid., Yaan city, Mingshan county, Mengding Mountain, 30°4ʹ35ʺN, 103°2ʹ29ʺE, 1251m elevation, 16 July 2023, on branches of Camellia sinensis ( Theaceae ), Y.H. Lu & X.D. Liang, MD33 (HUEST 23.0144), living culture UESTCC 23.0144; ibid., Guizhou province, Guiyang city, Huaxi county, 26°30ʹ43ʺN, 106°39ʹ32ʺE, 1155m elevation, 2 February 2023, on branches of Camellia sinensis ( Theaceae ), Y.H. Lu & Y.X. Yu, GY18 (HUEST 23.0143), living culture UESTCC 23.0143.

Notes: — Crassiparies was introduced with C. quadrisporus as the type by Li et al. (2016) and revised by Tanaka et al. (2017). Crassiparies quadrisporus occur in various plant species as saprobes and endophytes and is widely distributed in temperate and tropical regions ( Hongsanan et al. 2020). Our collection is morphologically similar to the original description of Crassiparies quadrisporus in Li et al. (2016), which has thick-walled ascomata, 4-spored asci and hyaline, broad fusiform ascospores. Based on the phylogenetic analyses, three strains (UESTCC 23.0134, UESTCC 23.0143 and UESTCC 23.0144) were grouped with the ex-type strain (MAFF 245408) and the other strain of C. quadrisporus (MAFF 246250) with statistical support (100% ML/1.00 BYPP) ( Fig. 1 ). Crassiparies quadrisporus has been reported on Acer sp. , Japan ( Li et al. 2016), Machilus japonica , Japan ( Tanaka et al. 2017), Hevea brasiliensis , Thailand ( Senwanna et al. 2021), Jasminum nudiflorum and Camellia sinensis , China (this study). We identified our three collections as C. quadrisporus and introduced it as a new geography ( China) and host record ( Camellia sinensis ).

Figures

FIGURE 1. Phylogenetic tree from ML analysis based on the combined ITS, SSU, LSU, RPB2, and TEF1-α sequences data. Bootstrap values for ML and MP ≥ 75% are placed above the branches. Branches with Bayesian posterior probabilities (BYPP) ≥ 0.95 are in bold. The tree is rooted to Cyclothyriella rubronotata (CBS 141486 and CBS 419.85). The ex-type strains were indicated in bold, and newly generated sequences were indicated in red.

FIGURE 4. Crassiparies quadrisporus (HUEST 23.0144) a Substrate. b, c Ascomata on host surface. d Vertical section of ascomata. e Peridium. f Hamathecium. g–l Asci. m–q Ascospores. r Colony on PDA. Scale bars: d = 100 µm, e = 25 µm, f–l = 20 µm, m–q = 10 µm.