Entomoneis sp.

Adalioğlu, Sezgi & Çalişkan, Gülizar, 2020, Effect of Nutrient, Light Intensity and Temperature on the Growth Rates and Metabolism of a Stress-Resistant Bacillariophyta Species Entomoneis sp. - in Izmir Bay (Aegean Sea) Abstract, Mediterranean Marine Science 21 (1), pp. 1-10 : 2-3

Isolation of Entomoneis sp.

Samples of the mixed marine algal cultures were viewed morphologically in a stereo microscope for initial identification. Monoalgal culture of Entomoneis sp. was

Medit. Mar. Sci., 21/1 2020, 1-10

isolated by serial dilution technique in the Class II Laminar Flow Biosafety Cabinet with using NIKON SMZ 25 that has high resolution and exceptionally bright specification.

Preparation of the Synthetic Seawater Medium and Stock Culture

The isolated culture from Izmir Bay was maintained in filtered and sterilized L1 medium which was suggested by Guillard & Hargraves (1993) with some modification of nitrogen and phosphate concentrations. Bioassay conditions were prepared under monoculture conditions and stock cultures of microalgae were inoculated into 300mL medium with 33±1 psu of artificial salinity in 500mL Erlenmeyer flasks. Then the microalgae stock cultures were incubated under a controlled temperature of 21±1 oC and illumination of 50μmol photons m-2 s-1 on the surface of the flasks and agitated by hand in a 10:14 dark:light regime. Sparging of the glass bottles was bubbled by filter-sterilized air (0.2 µm filter, Sartorius, Germany).