Trichodina reticulata Hirschman & Partesh 1955

Göçmen, Bayram, 2017, First record of ectoparasitic ciliates, of genus Trichodina (Ciliophora: Trichodinidae) parasiting cultured Oranda Gold Fish (Carassius auratus auratus L.) in India, Zootaxa 4319 (1), pp. 128-142 : 131

Trichodina reticulata Hirschman & Partesh 1955

( Fig 3A , 4A ; Table 1)

Description. (n=26) Body is big sized. In lateral view, it is disc shaped with a cell diameter of 50.0–74.5 (62.25±7.32) µm. Central area of an adhesive disc arranged with twelve equal shaped and spherical granules containing border membrane measuring 4.0–5.1 (4.5±0.7) µm in width with adhesive disc of 42.3–57.5 (49.9±3.78) µm in diameter. It contains denticlular ring measuring 27.9–36.0 (31.95±5.7) µm in diameter. The number of denticle is 25–31 (28±4.2). Denticle spans measuring 12.5–15.9 (14.2±2.4) µm. Length of denticle is 6.0–8.0 (7.0±1.4) µm. Blade broad and club-shaped. It is 2.5–8.0 (5.25±3.8) in length. Anterior blade surface straight and the lower part of anterior blade slightly curve in some specimens ( Figure 3a and 3b ). Central part consists of rounded overlapping area measures 2.0–3.0 (2.5±0.7) µm in diameter in width. Apex of the blade is projected towards Y+1 axis. Length of ray measures 5.5–7.0 (6.25±1.0) µm and number of radial pins per denticle is 8–10 (9±1.4). Nuclear apparatus not conspicuous.

Taxonomic summary

Host: Carassius auratus auratus (L.)

Fish Family: Cyprinidae

Locality : Howrah, West Bengal, India (22°35’N, 88°19'E)

Location: Gills

Reference material. Lectotype: In slide no. GF-TR-11/2014, deposited in the collection of the Parasitology Laboratory, Department of Zoology, University of Kalyani, Kalyani, Nadia, West Bengal, India. Remarks. On analyzing the morphmetric and quantitative data of the present specimen, it was readily identified as Trichodina reticulata Hirschman & Partesh (1955) . The species was originally described by Hirschmann (1955). It has been reported from different part of the world ( Lom 1960; Lom et al. 1976; Dove & Donoghue 2005; Tang & Zhao 2010; Hu 2012; Martin 2012). Trichodina reticulata is a highly distinctive species, clearly recognizable based on its denticles shape and granules present in the central area. T. reticulata has previously been recorded in India by Mishra and Das in 1993 from the gill of freshwater fish, Catla catla but it has been recorded for the first time as a pathogen of Carassius spp . in India and throws new light on the biodiversity and most importantly revealed a wide range of host preference, as it is the first record from Carassius auratus auratus (L.) which established as a new host for T. reticulata in India. The dimensions of the present material are showing huge similarities to those recorded in Florianópolis, Santa Catarina State, Brazil by Martins et al. (2012). ( Table 1)

Figures

FIGURE 3. A – F Photomicrographs of silver nitrate impregnated adhesive discs of Trichodinid species. (A) Trichodina reticulata Hirschman & Partesh 1955 (B) Trichodina mutabilis Kazubski & Migala 1968 (C) Trichodina acuta Lom 1961 (D) Trichodina ngoma Van & Basson 1992 (E) Trichodina nandusi Mitra et al. 2013 (F) Trichodina domergui Wallengren 1897 identified from Carassius auratus auratus (L.). Scale Bar – 20 µm.

FIGURE 4. A – F Diagrammatic drawings of the denticles of Trichodinid species i. e. (A) Trichodina reticulata Hirschman and Partesh 1955 (B) Trichodina mutabilis Kazubski & Migala 1968 (C) Trichodina acuta Lom 1961 (D) Trichodina ngoma Van & Basson 1992 (E) Trichodina nandusi Mitra et al. 2013 (F) Trichodina domergui Wallengren 1897 obtain in the present study.