Tubeufia hainanensis J. Ma & Y.Z. Lu, 2023

Ma, Jian, Zhang, Li-Juan, Boonmee, Saranyaphat, Xiao, Xing-Juan, Liu, Ning-Guo, Xiao, Yuan-Pin, Luo, Zong-Long & Lu, Yong-Zhong, 2023, Morphological and phylogenetic analyses reveal three new species and one new record of Tubeufia (Tubeufiales, Tubeufiaceae) from southern China, MycoKeys 99, pp. 87-108 : 87

Tubeufia hainanensis J. Ma & Y.Z. Lu sp. nov.

Fig. 3

Etymology.

The epithet ‘‘ Tubeufia hainanensis ’’ refers to the collecting site.

Holotype.

HKAS 125884.

Description.

Saprobic on decaying wood in moist ground. Sexual morph Undetermined. Asexual morph Hyphomycetous, helicosporous. Colonies on natural substrate superficial, effuse, gregarious, white. Mycelium superficial, partly immersed, hyaline to pale brown, septate, branched hyphae, smooth. Conidiophores macronematous, mononematous, straight to slightly flexuous, cylindrical, unbranched, septate, 44-56 μm long, 4-5 μm wide, pale brown to hyaline, smooth-walled. Conidiogenous cells holoblastic, mono- to polyblastic, integrated, sympodial, intercalary or terminal, cylindrical, with a denticulate protrusion, truncate at apex after conidial secession, 8-14 μm long, 3-5 μm wide (x̄= 9.5 × 4 μm, n = 20), hyaline to pale brown, smooth-walled. Conidia solitary, acropleurogenous, helicoid, rounded at tip, 16-21 μm diam conidial filament 1.5-4 μm wide (x̄ = 19 × 3 μm, n = 30), 127-175 μm long (x̄ = 144 μm, n = 30), indistinctly septate, coiled 31/2-33/4 times, becoming uncoiled in water, guttulate, hyaline, smooth-walled.

Culture characteristics.

Conidia germinating on water agar and producing germ tubes within 8 h. Colonies on PDA circular with umbonate surface and undulate edge. Growth rate 43 mm diam in 50 days at 25 °C, with a pale brown surface.

Material examined.

China, Hainan Province, Haikou City, Xiuying District, Ecological leisure trail, 20°01′N, 110°25′E, on decaying wood in terrestrial habitat, 10 August 2021, Jian Ma , HK 1 (HKAS 125884, holotype; GZAAS 22-2015, isotype), ex-type living cultures CGMCC, GZCC 22-2015; Ibid. , HK 1-2 (HKAS 125883, paratype), living culture GZCC 23-0589 .

Notes.

Tubeufia hainanensis resembles T. parvispora morphologically, with solitary, acropleurogenous, hyaline, helicoid conidia. However, Tubeufia hainanensis can be distinguished from T. parvispora by its unbranched conidiophores ( Lu et al. 2018b). Tubeufia hainanensis formed a sister clade to Tubeufiaceae (BBC 3512) with 85% ML/0.99 PP supports (Fig. 1 ), and the phylogeny indicated that it is distinct species.

Figures

Figure 1. Phylogenetic tree generated from maximum likelihood (ML) analysis based on a combined LSU, ITS, tef 1 α, and rpb 2 sequence data. The bootstrap support values of ML are equal to or greater than 75 %, and Bayesian posterior probabilities (PP) equal to or greater than 0.95 are given near the nodes as ML / PP, respectively. Acanthohelicospora aurea GZCC 16 - 0060 and A. guianensis UAMH 1699 were used as outgroup taxa. The new species are indicated in red bold and newly generated sequences are indicated in black bold. " T " denotes ex-type strain. Symbols after generic names in Tubeufia indicate the habitats of taxa as explained in the phylogram.

Figure 3. Tubeufia hainanensis (HKAS 125884, holotype) a, b colonies on the host surface c-e conidiophores, conidiogenous cells and conidia f-h conidiogenous cells bearing conidia j-o conidia i germinated conidium p, q colonies on PDA, p from above q from below. Scale bars: 20 μm (c-e, i-l); 10 μm (f-h, m-o).