Tubeufia muriformis J. Ma & Y.Z. Lu, 2023

Ma, Jian, Zhang, Li-Juan, Boonmee, Saranyaphat, Xiao, Xing-Juan, Liu, Ning-Guo, Xiao, Yuan-Pin, Luo, Zong-Long & Lu, Yong-Zhong, 2023, Morphological and phylogenetic analyses reveal three new species and one new record of Tubeufia (Tubeufiales, Tubeufiaceae) from southern China, MycoKeys 99, pp. 87-108 : 87

Tubeufia muriformis J. Ma & Y.Z. Lu sp. nov.

Fig. 4

Etymology.

The epithet ‘‘ Tubeufia muriformis ’’ refers to the multi-septate conidia of this taxon.

Holotype.

HKAS 128853.

Description.

Saprobic on decaying bamboo in a terrestrial habitat. Sexual morph Undetermined. Asexual morph Hyphomycetous, helicosporous. Colonies on natural substrate superficial, effuse, gregarious, white. Mycelium superficial, partly immersed, hyaline to pale brown, septate, branched hyphae, smooth, with masses of crowded, glistening conidia. Conidiophores macronematous, mononematous, straight or flexuous, simple, cylindrical, branched or unbranched, indistinctly septate, 13-36 μm long, 3.5-7.5 μm wide, hyaline, smooth-walled. Conidiogenous cells holoblastic, monoblastic, integrated, sympodial, terminal, cylindrical, truncate at apex after conidial secession, hyaline, smooth-walled. Conidia solitary, acrogenous, muriform, curved, 23-25 μm diam and conidial filament 11-15 μm wide (x̄ = 24 × 13 μm, n = 20), 16.5-58.5 μm long (x̄ = 49 μm, n = 20), composed of two rows of cells with pale, multi-septate, apical cells cylindrical, basal cells truncate, constricted at septae, tapering toward base and top of conidia, coiled 1/4-1 times, not becoming loose in water, guttulate, hyaline to pale brown, thick-walled, smooth-walled.

Culture characteristics.

Conidia germinating water agar and producing germ tubes within 12 h. Growth on PDA with a circular shape and umbonate surface and entire edge. Growth rate 42 mm diameter in 40 days at 25 °C with a pale brown surface.

Material examined.

China, Guizhou Province, Qianxinan Prefecture, Xianheping National Forest Park, 24°97′N, 105°63′E, on submerged decaying wood in a freshwater stream, 16 March 2022, Jian Ma, XHP38 (HKAS 128853, holotype; GZAAS 22-2039, isotype), ex-type living cultures CGMCC, GZCC 22-2039; Ibid., XHP64 (GZAAS 23-0594, paratype), living culture GZCC 23-0591 .

Notes.

Tubeufia muriformis shares morphological similarities with Xenosporium helicominum , characterized by the presence of mononematous, straight or flexuous conidiophores, monoblastic, terminal, cylindrical conidiogenous cells, and muriform, curved, hyaline to pale-brown conidia. However, T. muriformis can be distinguished from X. helicominum with its branched conidiophores and larger conidia (23-25 × 11-15 μm vs. 14-16.5 × 5.5-6.5 µm; Zhao et al. 2007). Moreover, T. muriformis differs from other Tubeufia species in that it has muriform, curved conidia ( Lu et al. 2018b).

The phylogenetic analysis indicated that Tubeufia muriformis formed a closely related clade with T. bambusicola , supported by ML bootstrap value of 100% and PP of 1.00. This phylogenetic relationship confirms that Tubeufia muriformis and T. bambusicola are distinct species, as demonstrated by the phylogenetic tree.

Figures

Figure 4. Tubeufia muriformis (HKAS 128853, holotype) a, b colonies on the host surface c-e conidiophores, conidiogenous cells and conidia d, f-i conidiogenous cells k-o conidia j germinated conidium p, q colonies on PDA, p from above q from below. Scale bars: 20 μm (c-f, j-k); 10 μm (g-i, l-o).