Kempnyia couriae, Avelino-Capistrano & Barbosa & Takiya, 2016

Avelino-Capistrano, Fernanda, Barbosa, Leandro Silva & Takiya, Daniela Maeda, 2016, Description of a new Kempnyia Klapálek from Brazil (Plecoptera: Perlidae) with life stages associated using DNA barcodes, Zootaxa 4079 (3) : -

Kempnyia couriae sp. nov.

Figs. 2–12 .

Male. General color yellowish ( Fig. 2 ). Compound eyes black. Three ocelli totally pigmented. Frons with brown spots, covered by long bristles; M-line evident, forming by light area on the frons ( Fig. 2 ). Epicranial suture surpassing paired ocelli. Antenna yellowish. Maxilary palpi pale. Pronotum rugose, brown and trapezoidal. Legs yellow; tibiae with brown bristles. Wings light brown, with veins 1–7 M-CuA and 1–6 CuA-CuP brown; R-vein brown; Sc brown ( Fig. 3–4 ). Ventral side pale. Sclerite X with brown bristles forming two separate areas.

Paraprocts digtiform, with apex pointed and ventrally curved. Cerci yellow, with many thin bristles on each segment. Hammer square, covered by bristles; ventral area ventrally projected ( Fig. 5 ). Penial armature brown; ring encompassing ½ of total size of structure; gonopore elongate, surpassing the hooks with apex forming globular and membranous structure, ventrally curved; hooks moderately long, curved ventrally ( Fig. 6–7 ).

Female. Color pattern similar to male ( Fig. 8 ). Subgenital plate simple and with median notch on posterior margin ( Fig. 9 ).

Nymph. General color brown ( Fig. 10 ). Head with pale areas forming an M-line; light spots in occipital area; prominent light area surrounding posterior ocelli; compound eyes black; three ocelli black. Antennae yellow. Pronotum rectangular. Pronotum, mesonotum, and metanotum brown, with light spots forming a characteristic color pattern ( Fig. 10 ). Legs light brown, with many bristles and spines covering the femora in anterior view ( Figs.

12–13). In posterior view, short bristles; thick spine-like setae present. Filamentous gills SC01. Abdomen brown, covered by many bristles; spines present on margin of each segment ( Fig. 11 ). Posterior margin of tergite X oval. Anal gills present. Cerci yellow, short bristles in anterior area; spine-like setae in each segment.

Measurements. Male: head— 2.2 mm; forewing 13.3–14.2 mm (n = 2). Female: Head: 2.39 mm; forewing: 14.99 mm (n =1). Mature nymphs: head: 2.67–2.74 mm; Wing pads: 2.57–2.74 mm (n = 3).

Distribution. Brazil: Rio de Janeiro, Casimiro de Abreu.

Etymology. This species in named in honor of Dr. Marcia Souto Couri, Dipterologist from Museu Nacional, Universidade Federal do Rio de Janeiro, who coordinated the project “Biota Diptera Fluminense”.

Type material. Holotype. Brazil. Rio de Janeiro. Casimiro de Abreu. Reserva BiolÓgica UniÃo, Trilha Interpretativa , Lago Artificial , CDC blue light, 27. iv. 2013, 1 ♂ ( DZRJ #3840*ENT1728 ), Col. F. Avelino- Capistrano & L.S. Barbosa. Paratypes. Reserva BiolÓgica UniÃo, Trilha Interpretativa, CÓrrego do PurgatÓrio, 28. i.2014, 1 ♀ ( DZRJ #3839* ENT 1729), CDC White light, Col. F. Avelino-Capistrano. & L.S. Barbosa. Same locality, 27. iv.2013, 1 ♂ ( DZRJ #3841* ENT 1728), CDC Blue light, Col. L.S. Barbosa. CÓrrego PurgatÓrio, Açude, 08. iii.2013, 3 nymphs ( DZRJ #3844* ENT 1726), Col. L. S. Barbosa. Same locality, 18–21. vii.2013, 6 nymphs ( DZRJ #3842), 21. iv.2013, 1n ( DZRJ #3847), Col. F. Avelino-Capistrano. & L.S. Barbosa. Same locality, 18– 21. vii.2013, 3 nymphs ( DZRJ #3843* ENT 1727), Col. F. Avelino-Capistrano & L. S. Barbosa. Same locality, 08.iii.2014. 1 ♂ ( DZRJ #3845* ENT 1730), 1 ♀ ( DZRJ #3846* ENT 1731), Malaise, Col. L. S. Barbosa & F. Avelino- Capistrano.

Taxonomy remarks. The male of K. couriae is characterized by the penial armature with the gonopore elongate, surpassing the hooks, and with an apex forming a globular and membranous structure, ventrally curved, hooks moderately long, and curved ventrally. This species is similar to K. tenebrosa KlapÁlek , especially in the morphology of the penial armature (see Zwick 1972, fig. 9 A – B). Kempnyia tenebrosa was described from females from ‘TheresÓpolis and subsequent authors considered that this material originated from Rio de Janeiro State (RJ) ( Froehlich 2010, 2011; Nessimian et al. 2009). However, Lecci (2013) revising the genus, stated that this locality, ‘TheresÓpolis is also an old name for the city of Águas Mornas, Santa Catarina State ( SC). Zwick (1972) illustrated two males from Brazil, one from CorupÁ (SC) and another from Tijuca (RJ), even though he observed differences in the length of the hooks and gonophores of these specimens, attributed in part to the teneral condition of the second specimen. Froehlich (2011) examining material from Blumenau deposited in Museu de Zoologia de SÃo Paulo ( MZSP) concluded that the material belongs to K. tenebrosa (Froehlich, fig. 5–6). Froehlich (2011) also examined a female of Nedenta fusca NavÁs, 1932 and concluded that this female was also K. tenebrosa . Froehlich (2011) additionally pointed out that the specimens from Rio de Janeiro are different from specimens from CorupÁ especially in the shape of the gonopore tube. The specimen noted by Zwick (1972, fig. 9A) based on specimen from Rio de Janeiro is similar to K. couriae , especially in the shape and length of the gonopore tube, the complete base of the penial ring, and by the protuberances at the base of the hooks. It is possible that this male may belong to the new species described herein. Unfortunately, Zwick (1972) did not illustrate the base of the penial ring of specimens from CorupÁ. Recently, Novaes & Bispo (2014, figs. 40–42) found an exemplar of K. tenebrosa in GrÃo ParÁ (SC) and their illustration are very similar to the figures presented by Zwick (1972) and Froehlich (2011). Unfortunately, specimens of K. tenebrosa were not included in our molecular analyses, preventing any speculation on the relationship of this species with K. couriae and other Kempnyia . Nevertheless, specimens of K. couriae from RBU are different from K. tenebrosa illustrated by these authors. Nymphs of K. couriae are very similar to nymphs of K. reticulata (KlapÁlek) , but are distinguished by head length, larger in K. reticulata (head length: 3.7mm—see Avelino-Capistrano et al. 2011) and smaller in the new species (head length: 2.74 mm).

Figures

FIGURES 2 – 7. Kempnyia couriae sp. nov. Male. 2. Head and pronotum. 3. Forewing. 4. Hindwing. 5. Sternum IX and X indicating the hammer. 6. Penial armature in lateral view. 7. Penial armature in ventral view.

FIGURES 8 – 13. Kempnyia couriae sp. nov. 8 – 9. Female. 8. Head and pronotum. 9. Sternum IX and X showing the subgenital plate. 10 – 13. Nymph. 10. Dorsal habitus. 11. Abdomen and cerci. 12. Posterior leg in anterior view. 13. Posterior leg in ventral view.