Neopestalotiopsis rhapidis Qi Yang & Yong Wang bis, 2021

Yang, Qi, Zeng, Xiang-Yu, Yuan, Jun, Zhang, Qian, He, Yu-Ke & Wang, Yong, 2021, Two new species of Neopestalotiopsis from southern China, Biodiversity Data Journal 9, pp. 70446-70446 : 70446

Neopestalotiopsis rhapidis Qi Yang & Yong Wang bis sp. nov.

Materials

Type status: Holotype. Occurrence: recordedBy: Qi Yang; occurrenceID: GUCC 21501; Taxon : scientificName: Neopestalotiopsis rhapidis; order: Amphisphaeriales ; family: Sporocadaceae ; genus: Neopestalotiopsis ; Location : country: China; stateProvince: Guangxi; locality: Nanning City , Guangxi Medicinal Botanical Garden ; verbatimCoordinates: 108°19' E, 22°51' N; Identification: identifiedBy: Qi Yang; dateIdentified: 2021; Record Level: collectionID: HGUP 332

Description

Disease symptom: Pathogenic causing spots on leaves tip of Rhapis excelsa . Leaf spots shape irregular, brown, slightly sunken on leaves tip. Small brown spots appeared initially and then gradually enlarged, changing to dark brown spots with a yellow border and jagged edge.

Colonies on PDA reach 7.5-8 cm in diam. after 7 d at room temperature (28°C), under light 12 hr/dark. Colonies filamentous to circular, whitish, with clustered black fruiting bodies and filiform and fluffy margin, white from above and light yellow from the reverse. Sexual morph: undetermined. Asexual morph（Fig. 1 ): Conidiomata 560-1405 µm in diam., pycnidial, globose, solitary, black, semi-immersed on PDA, exuding brown to dark brown conidia. Conidiophores branched or unbranched, hyaline, thin-walled. Conidiogenous cell discrete to lageniform, obclavate, hyaline or rarely light brown, smooth-walled. Conidia (22-)25.5 × 4(-6) µm (x̄ = 23 × 5.2 µm, n = 30), fusiform to clavate, straight to slightly curved, 4-septate; basal cell cylindrical to obconic, hyaline, thin-walled, smooth, 3-5 µm (x̄ = 3.7 µm, n = 30); the three median cells 11.5-15 µm (x̄ = 13.3 µm, n = 30), dark brown with septa darker than the rest of the cells, the second cell from base 3-5 µm (x̄ = 4 µm, n = 30); the third cell 2.5-6 µm (x̄ = 3.9 µm, n = 30); the fourth cell 3-4.5 µm (x̄ = 3.8 µm, n = 30); apical cell 2-4.5 µm (x̄ = 3.3 µm, n = 30), cylindrical, hyaline; 2-3 (mostly 3) tubular apical appendages, arising from the apex of the apical cell each at different points, flexuous, 11-16 µm (x̄ = 13.6 µm, n = 30); basal appendage present, single, tubular, unbranched, 2-5.5 µm (x̄ = 4 µm, n = 30).

Etymology

Latin, Neopestalotiopsis rhapidis , refers to the host plant ( Rhapis excelsa ) from which the fungus was isolated.

Notes

Neopestalotiopsis rhapidis clustered with N. cocoes (MFLUCC 15-0152) with 85% ML support, although without enough MP and BI support. Within comparison of the three gene regions, there were only three character differences in the ITS region, but 27 in the tef1 region. Neopestalotiopsis rhapidis has longer conidia and shorter apical appendages than those of N. cocoes (19-22.5 × 7.5-9.5 µm; 14.9-21 µm) ( Hyde et al. 2016). Thus, Neopestalotiopsis rhapidis (GUCC 21501) is introduced as a new species herein.

Figures

Figure 1. Neopestalotiopsis rhapidis (GUCC 21501). a. Leaf spots of Neopestalotiopsis rhapidis; b, c. Culture on PDA (b - above, c - reverse); d. Colony sporulating on PDA; e-g. Conidiophores; h-k. Conidia. Scale bars: d = 1000 µm, e-k = 20 µm.