Sutera cordata

Gousiadou, Chryssoula, Kokubun, Tetsuo, Albach, Dirk C., Gotfredsen, Charlotte H. & Jensen, Søren Rosendal, 2019, Iridoid glucosides in the genus Sutera (Scrophulariaceae) as chemotaxonomic markers in tribe Limoselleae, Phytochemistry 158, pp. 149-155 : 153

3.5. Sutera cordata

Fresh plants (43 g) were blended with EtOH (200 ml) and treated as above to give after concentration 1.57 g residue. Chromatography gave: a fraction containing mainly secologanoside (14, 520 mg), a fraction with mainly secologanic acid (13, 70 mg), a mixture (1:1) of sweroside (12) and villoside (11) (33 mg), echinacoside (16, 22 mg), followed by fractions (total 300 mg) which were repeatedly chromatographed over C 18 HPLC (MeCN-H 2 O) to yield: tubuloside A (18, MeCN–H 2 O 15:85, 2 mg) and 2′′′′-acetyl angoroside A (19, MeCN–H 2 O 21:79, 6 mg), both in a semi-pure state. Villoside (11, 5.9 mg) and sweroside (12, 6.0 mg) were separated by HPLC with MeCN-H 2 O (20:80). Pure samples of (13, 20.8 mg) and (14, 5.2 mg) were also obtained with MeCN-H 2 O gradient (17:83 → 85:15, v/v, over 15 min).