Yamadazyma paraaseri C.Y. Chai & F.L. Hui, 2021

Gao, Wan-Li, Li, Ying, Chai, Chun-Yue, Yan, Zhen-Li & Hui, Feng-Li, 2021, New species of Yamadazyma from rotting wood in China, MycoKeys 83, pp. 69-84 : 69

Yamadazyma paraaseri C.Y. Chai & F.L. Hui sp. nov.

Figure 4

Type.

China, Yunnan Province, Jinghong City, Mengyang Town , in rotting wood from a tropical rainforest, July 2018, K.F. Liu & Z.W. Xi (holotype NYNU 1811114T, culture ex-type CBS 16010 , CICC 33365) .

Etymology.

The species name Yamadazyma paraaseri refers to its phylogenetic similarity to C. aaseri .

Description.

The cells are ovoid to elongate (2-2.5 × 3-8.5 μm) and occur singly or in pairs after being placed in YM broth for three days at 25 °C (Figure 4A ). Budding is multilateral. After three days of growth on YM agar at 25 °C, the colonies are white to cream-colored, buttery, and smooth, with entire margins. After two weeks at 25 °C on a Dalmau plate culture with CM agar, pseudohyphae consisting of elongated cells with lateral buds are formed (Figure 4B ). True hyphae are not observed. Asci or signs of conjugation are not observed on sporulation media. Fermentation of sugars is absent. Glucose, galactose, l-sorbose, d-glucosamine, d-ribose, d-xylose, l-arabinose, d-arabinose, sucrose, maltose, trehalose, methyl α-d-glucoside, cellobiose, salicin, arbutin, lactose, melezitose, inulin, glycerol, erythritol, ribitol, d-glucitol, d-mannitol, d-gluconate, dl-lactate, succinate, citrate, and ethanol are assimilated. No growth is observed in l-rhamnose, melibiose, raffinose, xylitol, galactitol, myo -inositol, d-glucono-1, 5-lactone, 2-keto-d-gluconate, d-glucuronate, or methanol. In nitrogen-assimilation tests, growth is present on ethylamine, l-lysine, glucosamine, and d-tryptophan, while growth is absent on nitrate, nitrite, cadaverine, creatine, creatinine, and imidazole. Growth is observed at 37 °C but not at 40 °C. Growth in the presence of 0.01% cycloheximide, 10% NaCl with 5% glucose and 1% acetic acid is absent. Starch-like compounds are not produced. Urease activity and diazonium blue B reactions are negative.

Additional isolate examined.

China, Yunnan Province, Jinghong City, Mengyang Town, in rotting wood from a tropical rainforest, July 2018, K.F. Liu & Z.W. Xi, NYNU 181033.

GenBank accession numbers.

Holotype NYNU 1811114T (ITS: MK682794; D1/D2 LSU: MK682805); additional isolate NYNU 181033 (ITS: MZ318421; D1/D2 LSU: MZ318460).

Notes.

Two strains representing Y. paraaseri were clustered in a well-supported clade and were phylogenetically related to C. aaseri [7]. Yamadazyma paraaseri can be distinguished from C. aaseri based on ITS and D1/D2 LSU loci (8/573 in ITS and 8/531 in D1/D2 LSU). Physiologically, the ability to assimilate d-glucosamine and inulin and the inability to assimilate xylitol and d-glucono-1, 5-lactone are the primary differences between Y. paraaseri and its closest relative, C. aaseri . Additionally, C. aaseri can grow in 10% NaCl with 5% glucose, while Y. paraaseri cannot (Table 2 ) ( Lachance et al. 2011).

Figures

Figure 4. Yamadazyma paraaseri (NYNU 1811114, holotype) A budding cells after three days in YM broth at 25 ° C B pseudohyphae on cornmeal agar after two weeks at 25 ° C. Scale bars: 10 μm.