New enchytraeid species from Mount Hallasan (Jeju Island, Korea) (Enchytraeidae, Oligochaeta)
Author
Dózsa-Farkas, Klára
Author
Felföldi, Tamás
Author
Nagy, Hajnalka
Author
Hong, Yong
text
Zootaxa
2018
2018-10-05
4496
1
337
381
journal article
29257
10.11646/zootaxa.4496.1.27
e8ba8a68-c584-48d8-bfba-f4d2fd163e97
1175-5326
1446851
7C536E1E-5D5A-4E2D-9E4F-28F3CEA9664C
Xetadrilus jejuensis
sp. n.
(
Figures 12D
,
15–16
)
Type
material.
Holotype
:
NIBRIV0000810596, slide No. 2206, adult, stained whole mounted specimen.
Type
locality site 7,
Mt. Hallasan in Gwaneumsa
trail,
Jeju
Island,
Korea
, soil and litter layers of
Styrax japonica
tree, N 33˚23'15.9", E 126˚32'03.3",
1306 m
asl
, 0 9.06.2016, leg. Y. Hong.
Paratypes
(in total 13 stained, adult specimens on slides and
34 specimens
in 70% ethanol): NIBRIV0000810597, slide No. 2176, site 8, NIBRIV0000811386, slite No. 2396, site 8, P.119.1.1–119.1.8, slide No.
2203–2205
, 2207,
2399–2402
, from
type
locality, P.119.2.1– 119.2.2, slide No.
2397–2398
, site 8, P.119.3, slide No. 2300 (last 6 segments,
0.8 mm
for
DNA
analysis No. 1129). In 70% ethanol: P.119.4, twenty-three specimens,
type
locality; P.119.5 site 8 one specimen; P.119.6, site 6 one specimen; P.119.7, site 11 five specimens; P.119.8 site 12 four specimens.
FIGURE 15
. Micrographs of
Xetadrilus jejuensis
sp. n.
A.
Entire specimen.
B.
Head, lateral view (b=brain, vn=suboesophageal ganglion in II
–
IV, white arrow: prostomial recess; black arrows: prostomial papillae).
C.
Brain.
D.
Chaetae terminally.
E.
Clitellum, dorsal view (arrow: sperm funnel).
F.
Pharyngeal glands, dorsal view (p=primary dorsal lobes, sp=secondary ventral lobes, spermathecae marked with arrows).
G.
Preclitellar nephridia. A, C–D, F–G
in vivo
, B, E fixed, stained. Scale bars A 200 µm; B, E–G 50 µm; C, D 20 µm.
Further material examined
: 10 specimens, only
in vivo.
Etymology.
Named after
Jeju
Island.
Diagnosis.
The new species can be recognized by the following combination of characters: (1) small worms,
2.5–4.5 mm
long, width about 130–200 µm
in vivo
, segments 23–31; (2) lateral chaetae present in II–VII, absent from VIII on, formula: 2,0–0: 2–2; (3) clitellum saddle-shaped: dorsally granulocytes and hyalocytes, laterally only granulocytes; (4) in prostomium at the frontal tip present a vesicle-like recess, 6–8 inner papillae and a pair of prostomial ganglia; (5) brain incised posteriorly; (6) dorsal blood vessel from XII–XIII, blood colourless; (7) pharyngeal gland with unpaired dorsal lobes in IV and V, secondary ventral lobes in V and VI spherical, in VI–VII a pair of separate elongate lobes with a “Z-like fashion”; (8) two pairs of preclitellar nephridia in 6/7– 7/8; (9) sperm funnels 60–90 µm long
in vivo
, 2–3 times longer than wide, collar distinct; (10) male copulatory organs small, spherical glandular bulbs diameter 35–40 µm
in vivo
, extra bulbs absent; (11) spermathecae free, at the orifice of ectal ducts with a sessile gland, distally widening into a narrow ampulla, the following tubes widening into thin-walled ental reservoirs in VII or VIII.
FIGURE 16.
Micrographs of
Xetadrilus jejuensis
sp. n.
A.
Coelomocytes (arrows; i=intestine).
B.
Clitellum, ventral view (penial bulbs marked with arrows).
C.
Midgut pars tumida in XIX
–
XX (arrows).
D
,
F.
Sperm funnels (arrows) and sperm ducts.
E.
Sperm funnel and the brown coelomocytes.
G.
Spermatheca (long and thin arrows) and pharyngeal glands (black arrows: primary lobes; white arrows: secondary ventral lobes), dorsal view.
H.
Segment IV–VI, ventral view (p=primary pharyngeal lobes, white arrows: secondary ventral lobes; black arrows: spermathecae).
I.
Forepart of spermatheca with glands (ectal duct marked with white arrow, gland at the opening marked with black arrow). D–I
in vivo
, A–C fixed, stained. Scale bars A-D, F-H 50 µm, E, I 20 µm.
Description.
Small, slender worms (
Fig. 15A
).
Holotype
2.5 mm
long, 100 µm wide at VIII and 150 µm at clitellum (fixed), 23 segments. Length of
paratypes
2.5–4.5 mm
, width 130–180 µm at VIII and 170–200 µm at clitellum
in vivo
, length of fixed specimens
2.5–4 mm
, width 100–150 µm at VIII and 150–190 µm at clitellum, segments 23–31. Chaetae two per bundle (
Fig. 15D
), straight, slightly bent proximally 17–23 µm long and 2 µm thick preclitellarly, at the hindmost segments about 33–40 µm long and 2 µm thick. Lateral chaetae present in II– VII, absent from VIII on. Ventral chaetae from II on, absent in XII, formula 2,0–0: 2–2. On the body surface pale elongate glandular cells in 7–8 transverse rows and some roundish gland cells. Clitellum
XII–1
/2XIII, saddleshaped, not developed ventrally, the gland cells in about 18 transverse rows, dorsally granulocytes and hyalocytes but laterally and downwards towards the male copulatory organs only granulocytes (
Fig. 15E
). Prostomium similar to other
Xetadrilus
species, the head pore in mid-dorsal position on the prostomium (
Fig. 15B
). Frontal prostomial epithelium thickened, with a vesicle-like recess at the frontal tip. About 6–8 inner papillae and a pair of prostomial ganglions (15–22 µm long, fixed) present in prostomium (
Fig. 15B
). Prostomial musculature well developed. Body wall about 12–15 µm and cuticle 1–1.5 µm thick
in vivo
, septa
4/5– 9/10
thickened.
TABLE 2.
Comparison of the morphological characters of three
Xetadrilus
species recorded in different countries. Characters common to all four groups: 2 chaetae per bundle, lateral chaetae absent from VIII; pharyngeal glands in IV united, in V united + ventral lobes, VI in Z-fashion.
Xetadrilus jejuensis
sp. n.
(Korea)
|
Xetadrilus aphanoides
sp. n.
(Korea)
|
Xetadrilus aphanus
(Brazil)
*
|
Xetadrilus aphanus
(Korea)
|
Segment number (min-max) |
23–31 |
22–26 (34) |
19/23–28 |
18–28 |
Length (mm) |
2.5–4.5 |
2.8–4 |
2–3 |
3.2–4 |
Diameter at clitellum (µm) |
170–200 |
150–220 |
100–140 |
170–210 |
Length of preclitellar chaetae (µm) |
17–23 |
20–22 |
16–26 |
20–26 |
Length of terminal chaetae (µm) |
33–40 |
30–37 |
34–40 |
27–35 |
Intestine widen out in segments |
VII |
VIII |
VII–VIII |
VII–VIII |
Number of preclitellar nephridia |
2 |
3 |
2 |
2 |
Coelomocytes (µm; color: b=brown, p=pale) |
16–23, b |
18–25, p |
15–20, p |
17–21, p |
Midgut pars tumida (segment extension) |
XVIII–XXI (3) |
XVI–XX (2-3) |
1/2XVI–XXIII, (1.5– 4) |
? |
Length of sperm funnel (µm) |
60–90 |
30–48 |
50–60 |
60–90 |
Length: width ratio of sperm funnel |
2–3: 1 |
1.5–2: 1 |
1.3–1.5: 1 |
1.3–1.5: 1 |
Length of sperm (µm) |
30–50 |
30–35 |
42 |
37–42 |
Length of sperm heads (µm) |
12–15 |
10 |
18 |
15-17 |
Diameter of penial bulb (µm) |
35–40 |
30–38 |
20 |
29–35 |
Number and length of extra bulbs (µm) |
0 |
0 |
2×10 |
2×10–17 |
Spermatheca reaching into |
VII–VIII |
V–VI |
VII–VIII |
VII–VIII |
Diameter of spermathecal reservoir (µm) |
10–20 |
15-28 |
weakly developed |
10–13 |
* according to
Schmelz
et al.
2011
Brain about 90–100 µm long, 2 times longer than wide (fixed), incised posteriorly (
Fig. 15C
). Post-pharyngeal bulbs well developed. Perikarya of II–IV fused into suboesophageal ganglion, segmental ganglia present from V on (
Fig. 15B
). Pharyngeal glands (
Figs. 15F
,
16G–H
) with unpaired dorsal lobes in IV and V, primary ventral lobes in V elongate; secondary ventral lobes in V and VI spherical, smaller than primary ventral lobes. In VI–VII a pair of separate elongate lobes, consisting of an anterior dorsal part in VI and a posterior ventral part in VI–VII; both parts broadly connected in Z-like fashion (
Fig. 16G–H
). Oesophageal appendages and intestinal diverticula absent, intestine widening abruptly in VII. Chloragocytes small, light brown, about 10–13 µm long
in vivo.
Dorsal vessel from XII–XIII, blood colourless. Midgut pars tumida from XVIII–XXI, extending over 3 segment lengths (
Fig. 16C
). Two pairs of preclitellar nephridia from 7/8 to 8/9, not constricted at septums; anteseptale as long as postseptale or slightly shorter, terminal origin of efferent ducts (
Fig. 15G
), dorsal vesicle in the postseptale absent. First postclitellar nephridia at 13/14. Coelomocytes broadly oval, ca. 16–23 µm long, distinctly dark brown
in vivo
, filled with small refractile vesicle (
Fig. 16D,E
), in fixed specimens the cytoplasma pale with well visible nucleus, 12–19 µm long (
Fig. 16A
). Seminal vesicle absent. Sperm funnels 60–90 µm long
in vivo
2–3 times longer than wide (50–80 µm long and 2 times longer than wide, fixed), tapering distad, collar distinct, about as wide as funnel body or slightly narrower (
Figs. 15E
,
16D–F
). Sperm ducts 6 µm wide. Length of spermatozoa 30–50 µm, heads 12–15 µm
in vivo
(30–40 and 7–10 µm, fixed). Male copulatory organs (
Fig. 16B
) small, spherical glandular bulbs diameter 35–40 µm
in vivo
(25–30 µm, fixed) extra bulbs absent. Spermathecae (
Figs. 12D
,
16G–H
) not attached to oesophagus. Ectal ducts short (length 20–30 µm, diameter 12–15 µm
in vivo
) at the orifice with a sessile gland (
Fig.
16I
); distally widening into ampullae (diameter 16–17 µm), from here sperm present, which is arranged in parallel in longitudinal axis of spermatheca. The following tube narrower (diameter ca. 9 µm, 150–200 µm long
in vivo
), widening into thin-walled ental reservoir in VII or VIII (sometimes bent backwards into VI). The reservoir-sacks about 40–100 µm long and 10–20 µm wide
in vivo
and mostly filled with spheroid bodies (
Fig. 16G
). Mostly one large mature egg at a time.
Distribution and habitat.
Korea
, Mt. Hallasan,
Jeju
Island, site 6–8, 10–12: soil and litter layers under
Quercus mongolica
,
Styrax japonica
,
Quercus serrata
,
Sorbus alnifolia
and mixed forest.
Differential diagnosis
. Up to now, three
Xetadrilus
species have been reported with dark coelomocytes:
X. pitucus
(
Righi, 1974
)
,
X. righianus
(
Xie & Rota, 2001
)
and
X. maacki
Schmelz, Collado & Römbke, 2011
. But in
X. pitucus
, all three pharyngeal glands are united dorsally, the secondary lobes are absent and the lateral chaetae are absent already from VII. In
X. righianus
, the secondary lobes of pharyngeal glands also absent, the clitellar glands occur also ventrally but are weakly developed and the spermathecal reservoir is globular (diameter 37–40 µm). In
X. maacki
, all pharyngeal glands are united dorsally, three ventral pairs of secondary lobes are present and the spermathecae are confined to V. Similar and distinguishing traits of
Xetadrilus jejuensis
sp.n.
, X.
aphanoides
sp. n.
and
X. aphanus
are listed in
Table 2
.