New species of Yamadazyma from rotting wood in China
Author
Gao, Wan-Li
School of Life Science and Technology, Nanyang Normal University, Nanyang 473061, China
Author
Li, Ying
School of Life Science and Technology, Nanyang Normal University, Nanyang 473061, China
Author
Chai, Chun-Yue
School of Life Science and Technology, Nanyang Normal University, Nanyang 473061, China
Author
Yan, Zhen-Li
State Key Laboratory of Motor Vehicle Biofuel Technology, Henan Tianguan Enterprise Group Co., Ltd., Nanyang 473000, China
Author
Hui, Feng-Li
School of Life Science and Technology, Nanyang Normal University, Nanyang 473061, China
fenglihui@yeah.net
text
MycoKeys
2021
2021-08-26
83
69
84
http://dx.doi.org/10.3897/mycokeys.83.71156
journal article
http://dx.doi.org/10.3897/mycokeys.83.71156
1314-4049-83-69
B4422BDB86CA5F2599612CF8A18FB17F
Yamadazyma luoyangensis C.Y. Chai & F.L. Hui
sp. nov.
Figure 2
Type
.
China
,
Henan Province
,
Luoyang City
,
Song County
, in rotting wood from a forest park,
September 2020
,
J.Z. Li
&
Z.T Zhang
(
holotype
NYNU 201023T, culture ex-type
CBS 16666
, CICC 33509)
.
Etymology.
The species name
Yamadazyma luoyangensis
refers to the geographical origin of the type strain of this species.
Description.
The cells are ovoid to ellipsoid (2-4
x
3.5-7
μm
) and occur singly or in pairs after being placed in YM broth for three days at 25 °C (Figure
2A
). Budding is multilateral. After three days of growth on YM agar at 25 °C, the colonies are white to cream-colored, buttery, and smooth, with entire margins. After seven days at 25 °C on a Dalmau plate culture with CM agar, pseudohyphae are formed, but true hyphae are not (Figure
2B
). Asci or signs of conjugation are not observed on sporulation media. Glucose, galactose, trehalose, and cellobiose are fermented, but maltose, sucrose, melibiose, lactose, melezitose, raffinose, d-xylose, and inulin are not. Glucose, galactose, d-glucosamine, d-ribose, d-xylose, l-arabinose, d-arabinose, l-rhamnose, sucrose, maltose, trehalose, methyl
α-d-glucoside
, cellobiose, salicin, arbutin, melezitose, inulin, glycerol, erythritol, ribitol, d-glucitol, d-mannitol, galactitol, d-glucono-1, 5-lactone, 5-keto-d-gluconate, d-gluconate, succinate, citrate, and ethanol are assimilated. No growth is observed in l-sorbose, melibiose, lactose, raffinose,
myo
-inositol, 2-keto-d-gluconate, d-glucuronate, dl-lactate, or methanol. In nitrogen-assimilation tests, growth is present on ethylamine, l-lysine, glucosamine, and d-tryptophan, while growth is absent on nitrate, nitrite, cadaverine, creatine, creatinine, and imidazole. Growth is observed at 35 °C but not at 37 °C. Growth in the presence of 10% NaCl with 5% glucose is present, but growth in the presence of 0.01% cycloheximide and 1% acetic acid is absent. Starch-like compounds are not produced. Urease activity and diazonium blue B reactions are negative.
Figure 2.
Yamadazyma luoyangensis
(NYNU 201023, holotype)
A
budding cells after three days in YM broth at 25 °C
B
pseudohyphae on cornmeal agar after seven days at 25 °C. Scale bars: 10
μm
.
Additional isolate examined.
China, Henan Province, Luoyang City, Song County, in rotting wood from a forest park, September 2020, J.Z. Li & Z.T Zhang, NYNU 201035.
GenBank accession numbers.
Holotype NYNU 201023T (ITS: MW365549; D1/D2 LSU: MW365545); additional isolate NYNU 201035 (ITS: MZ318445; D1/D2 LSU: MZ318422).
Notes.
Two isolates representing
Y. luoyangensis
were resolved in a well-supported clade and are most closely related to
Y. mexicana
(Figure
1
).
Yamadazyma luoyangensis
can be distinguished from
Y. mexicana
based on ITS and D1/D2 LSU loci (4/592 in ITS and 10/531 in D1/D2 LSU). Physiologically,
Y. luoyangensis
differs from
Y. mexicana
by its ability to assimilate inulin and 5-keto-d-gluconate and its inability to assimilate lactose, raffinose, and 2-keto-d-gluconate. Additionally,
Y. mexicana
grows at 37 °C, while
Y. luoyangensis
does not (Table
2
) (
Kurtzman 2011
).