A species-level taxonomic review and host associations of Glyptapanteles (Hymenoptera, Braconidae, Microgastrinae) with an emphasis on 136 new reared species from Costa Rica and Ecuador Author Arias-Penna, Diana Carolina Author Whitfield, James B. Author Janzen, Daniel H. Author Winifred Hallwachs, Author Dyer, Lee A. Author Smith, M. Alex Author Hebert, Paul D. N. Author Fernandez-Triana, Jose L. text ZooKeys 2019 890 1 685 http://dx.doi.org/10.3897/zookeys.890.35786 journal article http://dx.doi.org/10.3897/zookeys.890.35786 1313-2970-890-1 FD8F695311F64DF2950F6A387340BCE5 2691DADB7BA352BEBA377C901FC0AC97 Glyptapanteles nataliaivanovae Arias-Penna, sp. nov. Figs 171 , 172 Female. Body length 2.53 mm , antenna length 3.33 mm , fore wing length 2.58 mm . Type material. Holotype : COSTA RICA • 1♀ ; 98-SRNP-16065.1, DHJPAR0012754; Area de Conservacion Guanacaste , Guanacaste , Sector El Hacha , Sendero Bejuquilla ; intergrade dry-rain forest; Malaise ; 280 m ; 11.03004 , -85.52699 ; 07.xii.1998 ; DH Janzen & W Hallwachs leg.; ( CNC ) . Paratypes . • 1 (0 ♀ , 0 ♂ ) (0 ♀ , 1♂ ); 98-SRNP-16164, DHJPAR0013624; same data as for holotype except: 23.xi.1998 ; ( CNC ) . • 1 (0 ♀ , 0 ♂ ) (0 ♀ , 1♂ ); 98-SRNP-16167, DHJPAR0013626; same data as for holotype except: 14.xii.1998 ; ( CNC ) . • 1 (0 ♀ , 1♂ ) (0 ♀ , 0 ♂ ); 98-SRNP-16169, DHJPAR0013628; same data as for holotype except: 14.xii.1998 ; ( CNC ) . • 1 (0 ♀ , 0 ♂ ) (0 ♀ , 1♂ ); 98-SRNP-16163, DHJPAR0013623; same data as for holotype except: 28.xii.1998 ; ( CNC ) . • 1 (0 ♀ , 0 ♂ ) (0 ♀ , 1♂ ); 98-SRNP-16166, DHJPAR0013625; same data as for holotype except: 28.xii.1998 ; ( CNC ) . • 1 (0 ♀ , 0 ♂ ) (0 ♀ , 1♂ ); 98-SRNP-16168, DHJPAR0013627; same data as for holotype except: 28.xii.1998 ; ( CNC ) . • 1 (0 ♀ , 0 ♂ ) (0 ♀ , 1♂ ); 99-SRNP-19242, DHJPAR0013629; same data as for holotype except: 18.i.1999 ; ( CNC ) . • 1 (0 ♀ , 0 ♂ ) (0 ♀ , 1♂ ); 99-SRNP-19243, DHJPAR0013630; same data as for holotype except: 18.i.1999 ; ( CNC ) . • 1 (0 ♀ , 0 ♂ ) (0 ♀ , 1♂ ); 99-SRNP-19245, DHJPAR0013632; same data as for holotype except: 18.i.1999 ; ( CNC ) . Diagnosis. Petiole on T1 parallel-sided in proximal half, then narrowing, finely sculptured ( Figs 171E, H , 172F ), propodeum without median longitudinal carina ( Figs 171G , 172E ), fore wing with vein 1 cu-a straight, r vein curved, outer side of junction of r and 2RS veins not forming a stub ( Fig. 172J ), dorsal outer depression on hind coxa present ( Figs 171A, J , 172A, I ), inner margin of eyes diverging slightly at antennal sockets ( Figs 171B , 172B ), and lateral grooves delimiting the median area on T2 clearly defined and reaching the distal edge of T2 ( Figs 171E, H , 172F ). Figure 171. Glyptapanteles nataliaivanovae sp. nov. female 98-SRNP-16065.1 DHJPAR0012754 A Habitus B, D Head B Frontal view D Dorsal view C Head, pronotum, propleuron, lateral view E T1-3, dorsal view F Mesonotum, dorsal view G Scutellum, metanotum, propodeum, dorsal view H, J Metasoma H Dorsal view J Lateral view I Mesosoma, lateral view. Figure 172. Glyptapanteles nataliaivanovae sp. nov. male 98-SRNP-16169 DHJPAR0013628, 07-SRNP-67011 DHJPAR0025549 A Habitus B, D Head B Frontal view D Dorsal view C Head, pronotum, propleuron, lateral view E Scutellum, metanotum, propodeum, dorsal view F T1-2, dorsal view G Mesosoma, lateral view H Metasoma, lateral view I Hind coxa, lateral view J, K Wings J Fore K Hind. Coloration ( Fig. 171 A-J ). General body coloration brown-black except scape yellow with a lateral brown band; pedicel, labrum, mandible and glossa yellow; first four proximal antennal flagellomeres dorsally lighter (light brown) than ventrally (dark brown), remaining flagellomeres dark brown on both sides; maxillary and labial palps, and tegulae ivory/pale yellow; propleuron proximal half yellow-brown/reddish and distal half yellow; central area of distal half on low face, ventral furrow of pronotum, distally dorsal furrow of pronotum, epicnemial ridge, and a small dot in the ventral edge of mesopleuron with yellow-brown/reddish tints. Eyes and ocelli silver. Fore and middle legs ivory/pale yellow and claws brown; hind legs yellow except coxae which distal half with unevenly brown/yellow-brown blotches, trochanter and trochantellus ivory/pale yellow, distally femora with a tiny brown dot, and distally tibiae and tarsomeres light brown. Petiole on T1 black and sublateral areas ivory/pale yellow; T2 with median and adjacent areas brown, adjacent area with contours well-defined, and lateral ends ivory/pale yellow; T3 broadly brown, proximally shape of the dark area coinciding with the width of median and adjacent areas on T2, but distally shape slightly wider, lateral ends with proximal half ivory/pale yellow and distal half with two bands: a brown one followed by a yellow-brown band; T4 and beyond completely brown; distally each tergum with a narrow yellowish transparent area. In lateral view, T1-2 completely ivory/pale yellow; T3 ivory/pale yellow, but dorsally brown; T4 and beyond completely brown. S1-3 yellow; S4 and beyond brown. Description. Head ( Fig. 171 A-D ). Head rounded with pubescence long and dense. Proximal three antennal flagellomeres length longer than wide (0.21:0.07, 0.22:0.07, 0.23:0.07), distal antennal flagellomere longer than penultimate (0.13:0.06, 0.10:0.06), antenna longer than body (3.33, 2.53); antennal scrobes-frons shallow. Face flat or nearly so, punctate-lacunose, interspaces wavy and longitudinal median carina present. Frons smooth. Temple wide, punctate-lacunose and interspaces wavy. Inner margin of eyes diverging slightly at antennal sockets; in lateral view, eye anteriorly convex and posteriorly straight. POL shorter than OOL (0.08, 0.10). Malar suture present. Median area between lateral ocelli slightly depressed. Vertex laterally pointed or nearly so and dorsally wide. Mesosoma ( Fig. 171A, F, G, I ). Mesosoma dorsoventrally convex. Mesoscutum proximally convex and distally flat, punctation distinct proximally with polished area distally, interspaces wavy/lacunose. Scutellum triangular, apex sloped and fused with BS , scutellar punctation distinct throughout, in profile scutellum flat and on same plane as mesoscutum, phragma of the scutellum partially exposed; BS only very partially overlapping the MPM ; ATS demilune with quite a little, complete and parallel carinae; dorsal ATS groove with semicircular/parallel carinae. Transscutal articulation with small and heterogeneous foveae, area just behind transscutal articulation smooth, shiny and depressed centrally. Metanotum with BM wider than PFM (clearly differentiated); MPM circular and bisected by a median longitudinal carina; AFM with a small lobe and not as well delineated as PFM ; PFM thick, smooth and with lateral ends rounded; ATM proximally with a groove with some sculpturing and distally smooth. Propodeum without median longitudinal carina, proximal half curved with medium-sized sculpture and distal half rugose; distal edge of propodeum with a flange at each side and without stubs; propodeal spiracle distally framed by a short concave carina; nucha surrounded by very short radiating carinae. Pronotum with a distinct dorsal furrow, dorsally with a narrow band; central area of pronotum and both dorsal and ventral furrows with sculpture. Propleuron with fine rugae and dorsally with a carina. Metasternum flat or nearly so. Contour of mesopleuron convex; precoxal groove deep with transverse lineate sculpture; epicnemial ridge elongated more fusiform (tapering at both ends). Legs. Ventral margin of fore telotarsus entire without seta, fore telotarsus almost same width throughout and longer than fourth tarsomere (0.10, 0.07). Hind coxa with punctation only on ventral surface, dorsal outer depression present. Inner spur of hind tibia longer than outer spur (0.26, 0.18), entire surface of hind tibia with dense strong spines clearly differentiated by color and length. Hind telotarsus longer than fourth tarsomere (0.13, 0.11). Wings ( Fig. 172J, K ). Fore wing with r vein curved; 2RS vein straight; r and 2RS veins forming a weak, even curve at their junction and outer side of junction not forming a stub; 2M vein straight; distally fore wing [where spectral veins are] with microtrichiae more densely concentrated than the rest of the wing; anal cell 1/3 proximally lacking microtrichiae; subbasal cell with microtrichiae virtually throughout; veins 2CUa and 2CUb completely spectral; vein 2 cu-a present as spectral vein, sometimes difficult to see; vein 2-1A proximally tubular and distally spectral, although sometimes difficult to see; tubular vein 1 cu-a straight, incomplete/broken and not reaching the edge of 1-1A vein. Hind wing with vannal lobe very narrow, subdistally and subproximally straightened, and setae evenly scattered in the margin. Metasoma ( Fig. 171A, E, H, J ). Metasoma laterally compressed. Petiole on T1 finely sculptured only laterally, parallel-sided in proximal half and then narrowing (length 0.30, maximum width 0.18, minimum width 0.10), and with scattered pubescence concentrated in the first distal third. Lateral grooves delimiting the median area on T2 clearly defined and reaching the distal edge of T2 (length median area 0.15, length T2 0.15), edges of median area polished and lateral grooves deep, median area broader than long (length 0.15, maximum width 0.17, minimum width 0.08); T2 with scattered pubescence only distally. T3 longer than T2 (0.25, 0.15) and with scattered pubescence only distally. Pubescence on hypopygium dense. Cocoon. Unknown. Comments. The fore tarsomeres are missing; the hind coxae are stout ( Fig. 171A ). Male ( Fig. 172 A-K ). The fore and middle legs have yellow trochanters, the trochantellus are ivory/pale yellow, proximally the coxae is darker than distally and the claws are brown; the hind legs are yellow except the distal half of the coxae which is black, the trochanter and the trochantellus are ivory/pale yellow, the femora distally have a tiny brown dot, the coloration of tibiae and tarsi is yellow/light yellow-brown. Etymology. Natalia Ivanova is (since 2004) the lead DNA scientist at Biodiversity Institute of Ontario ( BIO ), University of Guelph , Ontario , Canada . Distribution. Adult parasitoids were collected in Costa Rica , Sector El Hacha (Sendero Bejuquilla), during November-December 1998 and January 1999 at 280 m and 400 m in intergrade dry-rain and rain forests. Biology. Unknown. Host. Unknown.