New section and species in Talaromyces
Author
Sun, Bing-Da
China General Microbiological Culture Collection Center, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China & State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China
https://orcid.org/0000-0003-0272-6422
Author
Chen, Amanda J.
China General Microbiological Culture Collection Center, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China
https://orcid.org/0000-0001-5294-9574
Author
Houbraken, Jos
Westerdijk Fungal Biodiversity Institute, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands
Author
Frisvad, Jens C.
Department of Biotechnology and Biomedicine, Technical University of Denmark, Kongens Lyngby, Denmark
Author
Wu, Wen-Ping
Novozymes China, No. 14, Xinxi Rd, Shangdi, Beijing, China
Author
Wei, Hai-Lei
Key Laboratory of Microbial Resources Collection and Preservation, Ministry of Agriculture, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, China
https://orcid.org/0000-0001-6554-009X
Author
Zhou, Yu-Guang
China General Microbiological Culture Collection Center, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China
Author
Jiang, Xian-Zhi
Microbiome Research Center, Moon (Guangzhou) Biotech Ltd., Guangzhou 510535, China
jxz@moonbio.com
Author
Samson, Robert A.
Westerdijk Fungal Biodiversity Institute, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands
r.samson@wi.knaw.nl
text
MycoKeys
2020
68
75
113
http://dx.doi.org/10.3897/mycokeys.68.52092
journal article
http://dx.doi.org/10.3897/mycokeys.68.52092
1314-4049-68-75
BBED0AD34D38552BA75860CC3EDB4AEE
Talaromyces rufus B.D. Sun, A.J. Chen, Houbraken & Samson
sp. nov.
Fig. 11
Typus.
China
, Yunnan, soil, 2009, isolated by T.S. Zhou, Holotype CBS H-22832, culture ex-holotype CBS 141834 = DTO 349-D7 = CGMCC 3.13203.
Additional material examined.
Korea, soil, 2013, isolated by J. Houbraken, culture DTO 274-C5.
ITS barcode.
MN864272. Alternative identification markers:
BenA
= MN863341,
CaM
= MN863318,
RPB2
= MN863331.
Diagnosis.
This species produces red, determinate synnemata and ellipsoidal, spiny ascospores measuring 5-6
x
4-5
μm
.
In.
Talaromyces section Talaromyces
Colony diam, 7 d (mm).
CYA 12-16; CYA 30 °C 18-20; CYA 37 °C 15-16; MEA 37-38; MEA 30 °C 50-51; OA 38-40; YES 26-27; CREA Weak growth; CYAS No growth; DG18 9-13.
Colony characters.
CYA 25 °C, 7 d: Colonies deep, plane; margins entire; mycelium white and scarlet (5); texture floccose; sporulation sparse; conidia
en masse
greyish yellow-green (68); soluble pigments scarlet (5); exudates absent; reverse scarlet (5). MEA 25 °C, 7 d: Colonies moderately deep, plane; margins entire; mycelium white and scarlet (5); texture floccose; sporulation sparse; conidia
en masse
greyish yellow-green (68); soluble pigments scarlet (5); exudates absent; reverse scarlet (5). YES 25 °C, 7 d: Colonies moderately deep, raised at center, plane; margins entire; mycelium white and scarlet (5); texture floccose; sporulation absent; soluble pigments absent; exudates scarlet (5) droplets; reverse scarlet (5) at center, fading into peach (4). DG18 25 °C, 7 d: Colonies moderately deep, plane; margins entire; mycelium white; texture smooth and sticky; sporulation absent; soluble pigments absent; exudates absent; reverse cream white. OA 25 °C, 7 d: Colonies low, plane; margins entire; mycelium white and scarlet (5); texture floccose; sporulation absent; soluble pigments scarlet (5); exudates absent; reverse scarlet (5). Ascomata present. CREA 25 °C, 7 d: Acid production absent.
Micromorphology.
Conidiophores solitary and monoverticillate; stipes smooth, 5-30
x
2.5-3
μm
; phialides1-4, acerose, 10-12
x
3-4
μm
; conidia smooth, ellipsoidal to fusiform, 2.5-4.5
x
2-3
μm
. Ascomata maturing within 2-3 wk on OA, subglobose to ellipsoildal, 350-600
x
200-350
μm
, yellow, ascospores ellipsoidal, spiny, 5-6
x
4-5
μm
.
Notes.
Talaromyces rufus
is characterized by its red determinate synnemata on all tested media except DG18, CYAS and CREA. According to
Yilmaz et al. (2014)
, twelve
Talaromyces
species produce determinate or indeterminate synnemata, but
T. rufus
can be easily distinguished from them by its red synnemata. Phylogenetically,
T. rufus
is related to
T. macrosporus
; however,
T. macrosporus
produces broadly ellipsoidal ascospores and does not produce synnemata.
Etymology.
Latin,
rufus
, refers to its red synnemata.
Figure 11.
Talaromyces rufus
CBS 141834T
a
colonies from left to right (top row) CYA, MEA, YES and OA; (bottom row) CYA reverse, MEA reverse, DG 18 and CREA
b
synnemata on MEA after 1 wk incubation
c, d
conidiophores and conidia
e
ascomata
f, g
ascospores. Scale bars: 200
μm
(
b
), 10
μm
(
c, d, f
), 50
μm
(
e
), 2
μm
(
g
).