Neotropicomus indicus sp. nov.

(Fig. 1)

Neotropicomus indicus sp. nov. is characterized by basidiomata with a smooth to minutely pruinose, reddish brown pileus, stipe surface finely pruinose at the apex, downward to the middle with small reddish brown squamules, subfusoid to fusoid basidiospores, and a cutis type of stipitipellis interrupted by caulocystidia clusters. The species differs from N. australis by its smaller basidiospores, larger cheilocystidia and stipitipellis structure.

TYPE MATERIAL. — India • Kerala State, Thiruvananthapuram District, Palode; 6.VII.2022; holotype: ZGC[ZGCSN180]; GenBank: OQ633210 (ITS), OQ633194 (28 S) .

ETYMOLOGY. — “ indicus ” indicates the distribution and collection locality of the species.

GenBank accession numbersTaxonVoucherLocalityITS28S Austroboletus albidus Yan C. Li & Zhu L. YangHKAS107148, holotypeChina–NG088131 A. olivaceoglutinosus K.Das & DentingerHKAS57756China–KF112383 Boletus fagacicola B.Feng, Y.Y.Cui, J.P.Xu & Zhu L.YangHKAS55975, holotypeChinaJN563899JN563853 B. reticuloceps (M.Zang, M.S.Yuan & M.Q.Gong) Q.B.Wang & Y.J. Yao HKAS57671China–KF112454 B. bainiugan DentingerHKAS52235ChinaJN563905KF112457 B. edulis Bull.HMJAU4637RussiaJN563894KF112455 Brasilioporus olivaceoflavidus A.C.MagnagoFLOR 68467BrazilOM068901– B. olivaceoflavidusFLOR 68468BrazilOM068902– B. olivaceoflavidusVIES 9901322, holotypeBrazilOM068903– B. rufonigricans (T.W. Henkel) A.C.Magnago & T.W.HenkelG472GuyanaKJ786658– B. simoniarum A.C.MagnagoVIES 9901327, holotypeBrazilOM068905OM068914 Hortiboletus amygdalinus Xue T. Zhu & Zhu L. YangHKAS54166, holotypeChinaKT990581KT990777 H. subpaludosus (W.F.Chiu) Xue T. Zhu & Zhu L. YangHKAS59608ChinaKF112371– Imleria badia (Fr.) VizziniMB 03-098aUnited States–KF030355 I. badiaXb2Germany–KF030357 I. obscurebrunnea (Hongo) Xue T. Zhu & Zhu L. YangHKAS52557ChinaKC215207KF112374 Indoporus shoreae A.Parihar, K.Das, Hembrom & VizziniAP 6693, holotypeIndiaMK123972MK123973 I. shoreaeAP 6697IndiaMK123977MK123976 Neotropicomus australis A.C.MagnagoFLOR 68471BrazilOM068909– N. australisACM1138BrazilOM068907OM068916 N. australisVIES 9901328, holotypeBrazilOM068906– N. australisVIES 9901329BrazilOM068908OM068917 N. indicus sp. nov.ZGCSN180IndiaOQ633210OQ633194 N. parvogracilis (T.W.Henkel & Husbands) A.C.Magnago & T.W.HenkelTH9209, holotypeGuyanaJQ751261JQ751262 N. parvogracilisTH8850, paratypeGuyanaJQ751263HQ161865 Parvixerocomus aff. aokii (Hongo) G.Wu, N.K.Zeng & Zhu L. YangHKAS52633China–KF112379 P. aokiiHKAS59812China–KF112378 P. pseudoaokii G.Wu, K.Zhao & Zhu L. YangHKAS80480, holotypeChina–KP658468 Porphyrellus castaneus Yan C. Li & Zhu L. YangHKAS52554, holotypeChina–KT990697 P. castaneusHKAS63076China–KT990548 P. orientifumosipes Yan C. Li & Zhu L. YangHKAS53372, holotypeChina–KT990629 Strobilomyces echinocephalus Gelardi & VizziniHKAS59420China–KF112463 S. floccopus (Vahl) P. Karst.AFTOL-716United StatesAY854068AY684155 S. floccopusAFTOL-716United StatesAY854068AY684155 S. strobilaceus (Scop.) Berk.WU0016537Austria–KT990647 Tengioboletus glutinosus G.Wu & Zhu L. YangHKAS53425, holotypeChina–KF112341 T. glutinosusHKAS53452China–KT990655 T. reticulatus G.Wu & Zhu L. YangHKAS52241China–KT990657 T. reticulatusHKAS53453, holotypeChina–KT990656 Tylopilus dunensis A.C.Magnago & M.A.NevesMAN216, paratypeBrazilMF113418OM068920 T. dunensisMAN218, holotypeBrazilMF113419MF113428 T. felleus (Bull.) P. Karst.HKAS54926Germany–KF112411 T. felleusHKAS90203China–KT990545 T. violaceobrunneus Yan C. Li & Zhu L. YangHKAS89443, holotypeChina–KT990702 Xanthoconium purpureum Snell & E.A.DickNY00720964United States–KT990663 X. sinense G.Wu, Y.Y.Cui & Zhu L. YangHKAS77758, holotypeChina–KT990665 Xerocomellus corneri Xue T. Zhu & Zhu L. YangHKAS52503China–KT990668 X. communis Xue T. Zhu & Zhu L. YangHKAS50467China–KT990670 Xerocomellus sp.HKAS56311ChinaKF112340KF112170

HABITAT. — Solitary, on ground in forest dominated by Dipterocarpaceae members.

MYCOBANK. — MB848296.

DESCRIPTION

Basidiomata small-sized. Pileus 20-30 mm broad, convex when young, becoming plano-convex on maturity; surface viscid when wet, smooth to minutely pruinose, reddish brown to grayish brown towards the centre; margin straight. Pileal context whitish, unchanging when cut. Hymenphore adnate, depressed around the apex of stipe; surface pale yellow to olive yellow; pores 1-2 per mm, angular, unchanging on bruising; tubes 2-4 mm long, concolourous to the hymenophore surface, unchanging when cut. Stipe 20-40 × 3-4 mm, central, almost equal; surface finely pruinose at the apex downward to the middle with small reddish brown squamules, whitish to pale yellowish in apex, reddish brown in middle to bottom in a cream background. Stipe context whitish, unchanging on bruising. Basal mycelium white.

Basidiospores 9-11× 4-5µm (Q=1.8-2.75, Qm= 2.2), subfusoid to fusoid, thin- to slightly thick-walled (up to 0.5µm), smooth, yellowish in water,inamyloid.Basidia 20-27×7-10µm, clavate, thin-walled, 4-spored, hyaline with many oil droplets; sterigmata up to 3 µm long, hyaline with many oil droplets. Pleurocystidia 42-62×6-12 µm, narrowly fusiform to fusiform, thin-walled, hyaline, inamyloid.Cheilocystidia 28-50×7-10 µm, narrowly utriform, nearly fusiform, broadly clavate or nearly cylindrical, hyaline, inamyloid. Hymenophoral trama phylloporoid; hyphae 3-15µm wide, thin-walled, hyaline.Pileipellis a trichodermium, made up of densely packed chains of terminal elements formed on subglobose cells; terminal elements 16-31×4-13 µm, nearly cylindrical or broadly clavate, some of them having subacute apex, thin-walled, inamyloid. Pileus trama tightly interwoven; hyphae 3-12 µm wide, thin-walled, hyaline, inamyloid. Stipitipellis a cutis interrupted by caulocystidia clusters; caulocystidia 13-28× 4-7 narrowly clavate to clavate, rarely with subacute apex, thin-walled, inamyloid. Stipe trama parallel to interwoven; hyphae 3-12 µm wide, thin-walled. Clamp-connections absent.