Kockovaella iteae C. Y. Chai & F. L. Hui sp. nov.

Fig. 3

Etymology.

The specific epithet iteae refers to Itea, the plant genus from which the type strain was isolated.

Type.

China • Guizhou Prov.: Guiyang City, East Mountain Park, in the phylloplane of Itea yunnanensis, 15 Sept 2023, D. Lu, NYNU 239240 (holotype GDMCC 2.503 T preserved as a metabolically inactive state, culture ex-type PYCC 9996) .

Description.

On YM agar after 7 days at 20 ° C, the streak culture is white to cream-colored, butyrous, smooth and glistening, with an entire margin. After 7 days in YM broth at 20 ° C, cells are ellipsoidal or ovoid, 1.5–3.6 × 3.6–5.5 μm, single or pairs, and reproduced by polar budding and the formation of stalked conidia. The conidia are separated at the distal end of the stalks from parent cells. After 1 month at 20 ° C, a ring and sediment are present. In Dalmau plate culture on CMA, pseudohyphae are not formed. Sexual structures are not observed on PDA, CMA or V 8 agar. Ballistoconidia are symmetrical and apiculate, 1.8–2.4 × 2.7–3.3 μm. Glucose fermentation is absent. Glucose, inulin (delayed and weak), sucrose, raffinose, melibiose, galactose, lactose, trehalose, maltose, melezitose, cellobiose, salicin (delayed and weak), L-rhamnose, D-xylose, L-arabinose, D-arabinose (delayed), 5 - keto-D-gluconate (delayed and weak), D-ribose (delayed), erythritol (delayed), ribitol, galactitol, D-mannitol, D-glucitol, myo-inositol, succinate, citrate, D-glucosamine, N-acetyl-D-glucosamine, 2 - keto-D-gluconate (delayed), D-glucuronate, and glucono- 1.5 - lactone are assimilated as sole carbon sources. Methyl-α-D-glucoside, L-sorbose, methanol, ethanol, glycerol, DL-lactate, and D-gluconate are not assimilated. Ethylamine (delayed) and L-lysine are assimilated as sole nitrogen sources. Nitrate, nitrite, and cadaverine are not assimilated. Maximum growth temperature is 25 ° C. Growth in vitamin-free medium is positive. Growth on 50 % (w / w) glucose-yeast extract agar is negative. Starch-like substances are not produced. Urease activity is positive. Diazonium Blue B reaction is positive.

Additional strain examined.

China • Guizhou Prov.: Guiyang City, East Mountain Park, in the phylloplane of Itea yunnanensis, 15 Sept 2023, D. Lu, NYNU 239246 .

GenBank accession numbers.

holotype GDMCC 2.503 T (ITS: OR 958773, D 1 / D 2: OR 958772, RPB 1: PP 755337); additional strains NYNU 239246 (ITS: PP 752297, D 1 / D 2: PP 752296, RPB 1: PP 755338).

Note.

Physiologically, Kockovaella iteae sp. nov. differs from six closely related species, K. calophylli, K. cucphuongensis, K. litseae, K. quanzhouensis, K. schimae, and K. vietnamensis, in its ability to assimilate inulin and ethylamine (Table 3).

Species: 1, K. iteae; 2, K. schimae; 3, K. calophylli; 4, K. cucphuongensis; 5, K. litseae; 6, K. vietnamensis; 7, K. quanzhouensis; 8, K. sambucuse; 9, K. haikouensis; 10, K. ischaemi; 11, K. libkindii . +, positive reaction; –, negative reaction; d, delayed positive; w, weakly positive; n, data not available. All data from this study, except those marked with *, which were obtained from the original description (Lopandic et al. 2011; Takashima and Nakase 2011; Gomes et al. 2016).