Labrundinia johannseni Beck and Beck, 1966

(Figs 27–29)

Pentaneura pilosella Loew, 1866: Johannsen 1946 (part): 283 (description of male).

Labrundinia johannseni Beck and Beck, 1966: 342 (description adults and immatures); Roback 1971: 279 (description of male); Roback 1987: 196 (description of immatures).

Material examined. Type material: Holotype male with pupal and larval exuviae, USA: Florida, Flagler County, Rayonier Ditch, 30.i.1965, W. Beck (USNM).

Additional material: 1 larva, USA: Florida, Flagler County, Rayonier Ditch, 17.x.1967, W. Beck; 1 larva as previous except for 30.i.1965; 1 female with pupal exuviae as previous except for 27.ii.1965; 1 larva as previous except for Orange, Shinae Creek, 14.i.1958; 1 larva as previous except for Highlands, Arbuckle Creek, 3.ii.1987, R.P Rutter; 1 larva as previous except for Santa Rosa, Black river at Wright basin, 3.viii.1976, G. Bass. 1 male, Georgia, Decatur, Little Attapulgus Creek, Georgia Hwy 241, 7.xi.1984, B.A. Caldwell.

Diagnostic characters. Labrundinia johannseni differs from other Labrundinia species by the combination of the following characters. Adult male: abdominal tergites II, IV with continuous brown transverse band, T III over 75% brown, T V–VIII almost completely brown; hypopygium pale brown, sternapodeme with reduced anterior process. Pupa: Thoracic horn ovoid, about 2.07–2.20 times as long as wide; preapical indentation absent. Larva: Surface of head capsule smooth with brown transverse band, lateroventral spine group present with several conspicuous spines; subbasal seta of posterior parapod serrated; bifid claw with U-shaped lower indentation.

Description. Adult male (n = 2 unless otherwise stated)

Size. Total length 2.2–2.4 mm. Wing length 1.4–1.5 mm. Total length/wing length 1.50–1.73. Wing length/ profemur length 2.63 (1).

Coloration. Head brown with dark brown occipital margin; pedicel and antenna brown; maxillary palp pale brown. Thorax brown with scutum dorsally light brown; antepronotum pale yellow; supraalar callus pale brown. Wing membrane transparent without any marking. Legs pale. Abdominal tergites II, IV with continuous brown transverse band, T III over 0.75 brown, T V, VI–VIII almost completely brown; hypopygium pale brown (Fig. 27L).

Head (Figs 27A–E). Temporal setae 10 (1), uniserial (Fig. 27B). Eye ratio 0.94 (1). Tentorium (Fig. 27C) 171 (1) Μm long. Clypeus 85 (1) Μm long, 62 (1) Μm wide at largest part, bearing 24 (1) setae. Cibarial pump 213 (1) Μm long (Fig. 27D), with anterior margin concave. Lengths of palpomeres 1–5 (in Μm): 41 (1); 77 (1); 137 (1); 150 (1); 209 (1). Antennal with flagellum 692–813 Μm long, diameter of pedicel 116–118 Μm, apical setae single (Fig. 27E), AR 1.27–1.34.

Thorax. Antepronotum lateral setae not observed. Acrostichals 44–50, biserial, diverging evenly posteriorly, starting close to antepronotum and almost reaching scutellum; dorsocentrals 24–26, irregularly uniserial; prealars 12–15; supraalars 2–3. Anapleural suture ratio 0.45–0.46. Scutellum with transverse row of 10 setae and group of numerous fine anterior setae.

Wing (Fig. 27F). Width 0.4 mm. Costa 1.1–1.2 mm long. R2+3 present, but not very discernible. VR 0.74–0.75. WW 0.26–0.28. Brachiolum with 2 setae. Squama setiferous.

Legs (Figs 27G–K). Fore leg: width at apex of tibia 39–42 Μm (Fig. 27G), tibia with single, apical and pectinate spur 12–18 Μm long (Fig. 27H), with three lateral teeth and two preapical setae; preapical pseudospurs on ta1-4 not observed. Mid leg: width at apex of tibia 36–39 Μm long (Fig. 27I), tibia with single, apical and pectinate spur 22–23 Μm long with three lateral teeth (Fig. 27J) and two preapical setae; preapical pseudospurs on ta1-4 not observed. Hind leg: width at apex of tibia 37–427 Μm long (Fig. 27K), tibia without spur; comb with 5 bristles; preapical pseudospurs on ta1-4 not observed. Claws slender, hooked; pulvilli absent. Lengths and proportion of leg segments as in Table 17.

fe ti ta1 ta2 ta3 p1 399–524 429–549 313 234 180 p2 684–687 453–594 – – –

p3 547–590 664–810 547 273 195

ta4 ta5 LR BV SV p1 125 78 0.72 1.85 2.65 p2 – – – – –

p3 125 78 0.82 2.61 2.21 Hypopygium (Fig. 27M). Tergite IX arched, with 13 posterior setae. Membranous anal point rounded. Phallapodeme 53–56 Μm long. Sternapodeme with reduced anterior process. Gonocoxite cylindrical, 108–124 Μm long, 57 Μm wide, with slightly concave inner margin. GcR 1.90–2.19. Gonostylus simple and slender, 74–86 Μm long; megaseta 12–18 Μm long. HR 1.44–1.45. HV 2.77–2.92.

Adult female (n = 1 unless otherwise stated)

Size. Total length 1.2 mm. Wing length 1.2 mm. Total length/wing length 1.07. Wing length/ profemur length 3.45.

Coloration. Head, pedicel, antenna and maxillary palp pale brown. Thorax pale brown with scutum dorsally darker; antepronotum pale yellow; supraalar callus brown. Wing membrane transparent without any marking. Legs pale. Abdomen dark brown, not very discernible. Seminal capsules brown.

Head. Temporal setae uncounted, uniserial. Clypeus 57 Μm long, 48 Μm wide at largest part, setae uncounted. Cibarial pump 128 Μm long, as in male. Lengths of palpomeres 1–4 (in Μm): 34; 53; 99; 128; palpomeres 5 broken off. Antennal flagellum 278 Μm long, AR 0.33.

Thorax. Antepronotum lateral setae not observed. Acrostichals 30, irregularly biserial, starting close to antepronotum; dorsocentrals 34, irregularly uniserial; prealars 12; supraalars 2. Scutellum with transverse row of 10 setae and group of numerous fine anterior setae.

Wing. Width 0.4 mm. Costa 1.0 mm long. R2+3 not observed. VR 0.87. WW 0.32. Brachiolum with 2 setae. Squama setiferous.

Legs. Fore leg: width at apex of tibia 23 Μm, tibia with single, apical and pectinate spur 16 Μm long, with two lateral teeth and two preapical setae; preapical pseudospurs on ta1-4 not observed. Mid leg: width at apex of tibia 35 Μm, tibia with single, apical and pectinate spur 18 Μm long, with three lateral teeth and two preapical setae; preapical pseudospurs on ta1-4 not observed. Hind leg: width at apex of tibia 32 Μm, tibia without spur; comb setae not observed; preapical pseudospurs on ta1-4 not observed. Claws slender, hooked; pulvilli absent. Lengths and proportion of leg segments as in Table 18.

Genitalia. Cerci oval-quadrate. Seminal capsules oval, 38 Μm long, 21 Μm wide, with conical shaped necks.

Pupa (n = 2 unless otherwise stated)

Size. Abdomen 2.1 (1) mm long in male, 2.0 (1) mm long in female.

Coloration. Exuviae and thoracic horn brown. Apex of anal lobe brown.

Cephalothorax (Figs 28A–C). Frontal apotome smooth (Fig. 28A). Wing sheath smooth 0.9–1.0 mm long. Thoracic horn 221–258 Μm long and 106–117 Μm wide (Fig. 28B), THR 2.07–2.20, preapical indentation absent. Membranous preapical papilla 27 (1) Μm long (Fig. 28C), PTH 0.11 (1), aeropyle tube simple, short, not measurable; plastron plate small. Horn sac well developed, completely filling respiratory atrium. Reticulation of respiratory atrium indistinct. Basal lobe wedge-shaped. Thoracic comb with 10 conical tubercles (Fig. 28B).

Abdomen (Figs 28D–E). Tergite I with scar 129 (1) Μm long. T I–III without shagreen, T IV–V with a few spines medially concentrated, T VII–VIII with shagreen basally concentrated. Abdominal chaetotaxy as in figure 28D. Abdominal segment VII with 4 LS-setae. A VIII with 5 LS-setae. Anal lobe 255–257 Μm long and 140–162 Μm wide (Fig. 28E), outer margins sclerotized, with 10–12 spines, longest spine 12–15 Μm long, inner margins of lobes membranous. ALR 1.56–1.83. Male genital sac almost reaching apex of anal lobe.

4th instar larva (n = 7 unless otherwise stated)

Coloration. Head pale yellow, with brown transverse band; postoccipital margin brown. Second antennal segment brown; distal tooth of mandible and apex of ligula brown. Abdomen pale yellow; procercus and anal setae pale brown. Posterior parapod claws all pale yellow.

Head (Fig. 29A). Length 490 (1) Μm, 315 (1) Μm wide. IC 0.62–0.72. Surface smooth; lateroventral spine group present, well developed, with 10–16 spines; posteroventral spine group absent. Ventral cephalic setae S9, S10 and SSm forming a straight line diagonal to longitudinal axis of head capsule (Fig. 29A).

Antenna (Figs 29B–C). Length 246–288 Μm, A1 167–198 Μm long, with ring organ placed 0.82–0.94 (4) from base, A2 69–78 Μm long. AR 2.13–2.42. Blade longer than A2 over-reached by accessory blade.

Maxilla (Fig. 29D). Basal palp segment 20–32 Μm long and 7–11 Μm wide at the middle, with ring organ 0.67– 0.83 from base. PR 1.78–3.21. APR 5.98–9.25.

Mandible (Fig. 29E). Length 60–69 Μm. Sensillum campaniformium 0.64–0.79 from apex, basal tooth bifid, with seta subdentalis projecting from sloping end towards apical tooth, accessory tooth present, AMD 3.28.

Mentum and M appendage . Dorsomental teeth reduced; pseudoradula uniformly granulate.

Hypopharyngeal complex (Figs 29F–G). Ligula 55–66 Μm long, 25–28 Μm wide, with row of 5 teeth. IO

0.80–0.98, MO 1.05–1.08. Paraligula bifid, 24–31 Μm long, inner tooth 19–25 Μm long, shorter than outer tooth. Pecten hypopharyngis with 7 teeth almost equal in size.

Body (Figs 29H–I). Without lateral fringe. Anterior parapods with simple claws. Procercus 137–170 Μm long, 31–43 Μm wide, with 7 anal setae 511–598 (6) Μm long. L/ W 3.60 –5.42. Supraanal seta well developed. Anal tubules 137–176 (3) Μm long. Posterior parapod 343–370 (5) Μm long; subbasal seta on posterior parapod serrated, with 7–9 spines basally (Fig. 29H); parapod apex with numerous simple claws; bifid claw with U-shaped lower indentation (Fig. 29I). B/C 0.86–0.98 (6).

Remarks. Adult males of Labrundinia johannseni appear most similar to those of L. neopilosella and L. setosa in having a distinct continuous transverse band on abdominal tergite II. However, the abdominal tergite III is more than 75% brown in L. johannseni while it has a continuous transverse band in L. neopilosella and L. setosa . The pupae of L. johannseni are very similar to those of L. neopilosella and L. longipalpis, but can be distinguished from these species by the thoracic horn apical width, which is about 1.5 times as long as basal width. The distinctive ventral maculation on the head capsule distinguish the larvae of L. johannseni from any known Labrundinia species. Labrundinia johannseni is a moderately abundant in the southeast of the United Stated. The larvae of this species has been collected in creeks and unpolluted pools.