GINT BANFASAE

In this species, we detected intrapopulation variability in diploid number and/or multivalent associations showing the presence of three cytotypes. The position of a single pair of 18S rDNA sites, as well as the 18S rDNA-bearing chromosome pair involved in the multivalent chain, are consistent in all cytotypes.

Cytotype I (2 ƋƋ; S1531, S1534) has 2 n = 18 chromosomes, which decrease in length from 9.85% to 1.81% of DSL (Supporting Information, Fig. S1A; Table S2). In post-pachytene nuclei, the chromosomes form seven bivalents and one quadrivalent that is composed of the four larger chromosomes (Fig. 2A). A single pair of 18S rDNA sites is located in the subterminal region of the chromosomes 3 and 4 involved in the quadrivalent (Fig. 2B; Supporting Information, Fig. S1A).

Cytotype II (1 Ƌ; S1532) exhibits 2 n = 18 chromosomes, which decrease in length from 9.79% to 1.77% of DSL (Supporting Information, Fig. S1B; Table S2). The post-pachytene cells show six bivalents and one hexavalent (chromosomes 1, 2, 3, 4, 15 and 18) (Fig. 2C; Supporting Information, Fig. S1B). A pair of 18S rDNA sites is located in the subterminal region of the chromosomes 3 and 4 involved in the hexavalent (Fig. 2D).

Cytotype III (2 ƋƋ; S1530, S1533) has 2 n = 19 chromosomes, which decrease in length from 9.66% to 1.78% of DSL (Fig. 2E; Supporting Information, Fig. S1C; Table S2). Post-pachytene spermatocytes exhibit five bivalents, one trivalent (chromosomes 7, 17 and 18) and one hexavalent (chromosomes 1, 2, 3, 4, 14 and 19). A pair of 18S rDNA sites is situated in the subterminal region of the chromosomes 3 and 4 involved in the hexavalent (Fig. 2F).