5.2. Inoculation of C. annuum with P. capsici and plant pathogenicity

Inoculation trials with P. capsici isolates on six different resistant and susceptible C. annuum accessions were conducted under greenhouse conditions (27 ± 1 ◦ C), with 16 h-light photoperiod and 65 % relative humidity according to method described by Parada-Rojas and Quesada-Ocampo (2018) and Nasr Esfahani (2018a, 2018b). The experiments were arranged in a completely randomized design in 100 replications in a single seedling nursery tray for every genotype filled with substrate of oven-sterilized mixture of sand-peat moss in equal parts. Nutrients to support plant growth were supplied through irrigation with Knop’ s nutrient solution (10 mg FeCl 3; 0.25 g KH 2 PO 4; 0.25 g KNO 3; 0.25 g MgSO 4 •7H 2 O and 1 g NaNO 3 ∙ L 1 of tap water) from Columbus Chemical Industries (Columbus, WI 53925) (Tehrani et al., 2020).