Two samples of

Disynaphia spathulata (Hook. & Arn.) R.M.King & H. Rob.

(syn. Eupatorium spathulatum,

common name: “macelinha”) (King and Robinson, 1987; Matzenbacher, 1979),

a sub-shrub up to 1.5 m tall, were obtained from the municipality of Canela, RS. The VO yields for the two samplings were similar despite their different vegetative stages (Table 1 and Table S1). In regard the chemical composition of the VOs of this species, the inflorescences and leaves of the first sample (Canela/05, April) showed very similar chemical compositions with a predominance of hydrocarbon monoterpenes (56.8–58.9%), especially α- pinene (7.0–11.6%), β- pinene (14.9–24.1%), limonene (12.2–13.1%), and (E)-β- ocimene (13.3–2.3%). In the sesquiterpene fraction, three main compounds were identified in the flowering period: The hydrocarbon sesquiterpenes β- caryophyllene, germacrene D, and bicyclogermacrene. However, the second sampling in the vegetative stage (Viamao/05, October) presented a very different quantitative pattern, in which oxygenated sesquiterpenes (64.1%), spathulenol (22.6%), caryophyllene oxide (7.0%), and α- cadinol (12.0%) were observed.