Chironomus acerbiphilus Tocunaga

The chromosome set is 2n = 8. Chromosome AE is submetacentric, chromosomes CD and BF are metacentric and chromosome G is telocentric (Fig.13). The centromere regions of all chromosomes are formed of large bands which look like a block of dark bands. Chromosome arm A has well developed NOR, while chromosome G has one BR. On both sides of the BR in chromosome G there are several dark bands which can be used for identification of the species. The species is a synonym of C. crassimanus (Barna et al. 2010) . Nineteen individuals were studied.

Collection sites: Plovdiv: fish pool, Sofia: South Park, Plovdiv: Mariza River .