Chironomus anthracinus Zetterrstedt

Chromosome set of the species is 2n = 8. Two metacentric chromosomes (AB and CD), one submetacentric—EF and one telocentric chromosome—G. The centromere regions of the chromosomes are characterised by distinct dark bands (Fig. 4). In the Bulgarian populations, the band sequences in arm A are in homozygous inverted sequences A2.2 (Fig. 4), and there is a heterozygous inversion in the arm D–D1.2. Chromosome G is very small and has one NOR and one BR, located at both ends of the chromosome. The dark band between them is a species marker (Fig. 4). Eight individuals were studied.

Collection sites: Sofia: South Park, Kardjale Dam .