taxonID	type	format	identifier	references	title	description	created	creator	contributor	publisher	audience	source	license	rightsHolder	datasetID
F34966167B0DFFBAFF45FA82FDC66D1A.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/11093380/files/figure.png	https://doi.org/10.5281/zenodo.11093380	FIG. 2. — Growth and fertility of Podospora anserina (Rabenh.) Niessl on glycerol media. The plates were inoculated at the center with a mat+/mat- heterokaryon and incubated for three weeks at 27°C in the presence of constant light, at which time pictures were taken. Arrowheads point toward ascospore-containing perithecia.See also Figure 4 for a dark field picture of the wild-type strain growing on M0 and M0 + 0.05 M glycerol.Top, Petri plate (Ø= 8 cm).Scale bars: 200 µm.	FIG. 2. — Growth and fertility of Podospora anserina (Rabenh.) Niessl on glycerol media. The plates were inoculated at the center with a mat+/mat- heterokaryon and incubated for three weeks at 27°C in the presence of constant light, at which time pictures were taken. Arrowheads point toward ascospore-containing perithecia.See also Figure 4 for a dark field picture of the wild-type strain growing on M0 and M0 + 0.05 M glycerol.Top, Petri plate (Ø= 8 cm).Scale bars: 200 µm.	2024-04-29	Gautier, Valérie;Nguyen, Tinh-Suong;Silar, Philippe		Zenodo	biologists	Gautier, Valérie;Nguyen, Tinh-Suong;Silar, Philippe			
F34966167B0DFFBAFF45FA82FDC66D1A.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/11093382/files/figure.png	https://doi.org/10.5281/zenodo.11093382	FIG. 3. — Toxicity of glycerol. M2 and M0 Plates containing the indicated concentration of glycerol (gly) and sorbitol (sorb) were inoculated at the center with a mat+/mat- heterokaryon and incubated for two weeks at 27°C in the presence of constant light, at which time pictures were taken.Perithecia are visible as small black dots. On the M2 medium, the presence of 0.1 M of glycerol inhibited growth and abolished fertility;higher concentrations of glycerol resulted in no growth. On the contrary, Podospora anserina (Rabenh.) Niessl grew and was fertile even in M2 medium containing the highest concentration of sorbitol. Podospora anserina was also fertile on medium containing sorbitol as sole carbon source at all concentrations, while it produced no visible perithecia on a medium with the same glycerol concentrations after two weeks.	FIG. 3. — Toxicity of glycerol. M2 and M0 Plates containing the indicated concentration of glycerol (gly) and sorbitol (sorb) were inoculated at the center with a mat+/mat- heterokaryon and incubated for two weeks at 27°C in the presence of constant light, at which time pictures were taken.Perithecia are visible as small black dots. On the M2 medium, the presence of 0.1 M of glycerol inhibited growth and abolished fertility;higher concentrations of glycerol resulted in no growth. On the contrary, Podospora anserina (Rabenh.) Niessl grew and was fertile even in M2 medium containing the highest concentration of sorbitol. Podospora anserina was also fertile on medium containing sorbitol as sole carbon source at all concentrations, while it produced no visible perithecia on a medium with the same glycerol concentrations after two weeks.	2024-04-29	Gautier, Valérie;Nguyen, Tinh-Suong;Silar, Philippe		Zenodo	biologists	Gautier, Valérie;Nguyen, Tinh-Suong;Silar, Philippe			
F34966167B0DFFBAFF45FA82FDC66D1A.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/11093378/files/figure.png	https://doi.org/10.5281/zenodo.11093378	FIG. 1. — Scheme of glycerol import and catabolism in Podospora anserina (Rabenh.) Niessl as transposed from pathways known in Saccharomyces cerevisiae Meyen ex E.C.Hansen, Schizosaccharomyces pombe Lindner, and Aspergillus nidulans G.Winter.	FIG. 1. — Scheme of glycerol import and catabolism in Podospora anserina (Rabenh.) Niessl as transposed from pathways known in Saccharomyces cerevisiae Meyen ex E.C.Hansen, Schizosaccharomyces pombe Lindner, and Aspergillus nidulans G.Winter.	2024-04-29	Gautier, Valérie;Nguyen, Tinh-Suong;Silar, Philippe		Zenodo	biologists	Gautier, Valérie;Nguyen, Tinh-Suong;Silar, Philippe			
F34966167B0DFFBAFF45FA82FDC66D1A.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/11093396/files/figure.png	https://doi.org/10.5281/zenodo.11093396	APPENDIX 2. — Phylogenetic analysis of NDGD. The tree was rooted with the divergent protein encoded by Pa_7_11100. The AN7193 protein defined as a NDGD is in red and the Podospora anserina (Rabenh.) Niessl proteins are in blue.	APPENDIX 2. — Phylogenetic analysis of NDGD. The tree was rooted with the divergent protein encoded by Pa_7_11100. The AN7193 protein defined as a NDGD is in red and the Podospora anserina (Rabenh.) Niessl proteins are in blue.	2024-04-29	Gautier, Valérie;Nguyen, Tinh-Suong;Silar, Philippe		Zenodo	biologists	Gautier, Valérie;Nguyen, Tinh-Suong;Silar, Philippe			
F34966167B0DFFBAFF45FA82FDC66D1A.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/11093384/files/figure.png	https://doi.org/10.5281/zenodo.11093384	FIG. 4. — Phenotype of the G3P mutants. Growth and fertility on M2 were evaluated eight days after inoculation of mat+/mat- heterokaryons of the indicated strains.For M2, the top plates are cultures on the M2 medium;the bottom ones are the cover of the same plates onto which ascospores seen as tiny black dots were expelled. Growth on M0, M0+0.05 M glycerol, and M0+ 0.1 M glycerol was evaluated after four days of growth.	FIG. 4. — Phenotype of the G3P mutants. Growth and fertility on M2 were evaluated eight days after inoculation of mat+/mat- heterokaryons of the indicated strains.For M2, the top plates are cultures on the M2 medium;the bottom ones are the cover of the same plates onto which ascospores seen as tiny black dots were expelled. Growth on M0, M0+0.05 M glycerol, and M0+ 0.1 M glycerol was evaluated after four days of growth.	2024-04-29	Gautier, Valérie;Nguyen, Tinh-Suong;Silar, Philippe		Zenodo	biologists	Gautier, Valérie;Nguyen, Tinh-Suong;Silar, Philippe			
F34966167B0DFFBAFF45FA82FDC66D1A.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/11093386/files/figure.png	https://doi.org/10.5281/zenodo.11093386	FIG. 5. — The PaGut1Δ and PaGUT2Δ mutants are sterile on glycerol media. Small but mature perithecia can be seen at the periphery of the colony when the wild type is incubated for 20 days on media containing 0.02 M glycerol, while they cannot be seen in the PaGut1Δ, PaGUT2Δ, and PaGut1Δ PaGUT2Δ mutants. Bottom, enlargement of representative fruiting bodies. Scale bars: 250 µm.	FIG. 5. — The PaGut1Δ and PaGUT2Δ mutants are sterile on glycerol media. Small but mature perithecia can be seen at the periphery of the colony when the wild type is incubated for 20 days on media containing 0.02 M glycerol, while they cannot be seen in the PaGut1Δ, PaGUT2Δ, and PaGut1Δ PaGUT2Δ mutants. Bottom, enlargement of representative fruiting bodies. Scale bars: 250 µm.	2024-04-29	Gautier, Valérie;Nguyen, Tinh-Suong;Silar, Philippe		Zenodo	biologists	Gautier, Valérie;Nguyen, Tinh-Suong;Silar, Philippe			
F34966167B0DFFBAFF45FA82FDC66D1A.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/11093388/files/figure.png	https://doi.org/10.5281/zenodo.11093388	FIG. 6. — Production of perithecia by Podospora anserina (Rabenh.) Niessl in the presence of Paenibacillus validus (Nakamura) Ash et al. and Escherichia coli T.Escherich. The presence of large amounts of mature perithecia (the black dots) are seen around and farther away from the bacterial colony on media containing 0.02 M glycerol. The fungus formed a dense mycelium on a plate containing 0.1 glycerol and the bacteria. Note that at 0.1 M glycerol, P. anserina remained sterile even in the presence of bacteria. Scale bars: 250 µm.	FIG. 6. — Production of perithecia by Podospora anserina (Rabenh.) Niessl in the presence of Paenibacillus validus (Nakamura) Ash et al. and Escherichia coli T.Escherich. The presence of large amounts of mature perithecia (the black dots) are seen around and farther away from the bacterial colony on media containing 0.02 M glycerol. The fungus formed a dense mycelium on a plate containing 0.1 glycerol and the bacteria. Note that at 0.1 M glycerol, P. anserina remained sterile even in the presence of bacteria. Scale bars: 250 µm.	2024-04-29	Gautier, Valérie;Nguyen, Tinh-Suong;Silar, Philippe		Zenodo	biologists	Gautier, Valérie;Nguyen, Tinh-Suong;Silar, Philippe			
F34966167B0DFFBAFF45FA82FDC66D1A.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/11093390/files/figure.png	https://doi.org/10.5281/zenodo.11093390	FIG. 7. — Production of mycelium and perithecia in the presence of Escherichia coli T.Escherich by Podospora anserina (Rabenh.) Niessl wild-type and mutant strains. The plates were inoculated at the same time at the center by a mat+/mat- heterokaryon of the fungus with the indicated genotypes, alone or with E. coli DH5α at the position indicated by the blue dots on the right and left of the center (visible on the M0 plates). The plates were incubated for 20 days at 27°C and constant illumination,after which they were photographed.Escherichia coli promoted the growth of the mycelium (clearly visible on plates containing 0.1 M glycerol) for all strains, and development of perithecia (clearly visible as small black dots on plates containing 0.05 M glycerol) in all strains except for the PaGut2Δ mutant.	FIG. 7. — Production of mycelium and perithecia in the presence of Escherichia coli T.Escherich by Podospora anserina (Rabenh.) Niessl wild-type and mutant strains. The plates were inoculated at the same time at the center by a mat+/mat- heterokaryon of the fungus with the indicated genotypes, alone or with E. coli DH5α at the position indicated by the blue dots on the right and left of the center (visible on the M0 plates). The plates were incubated for 20 days at 27°C and constant illumination,after which they were photographed.Escherichia coli promoted the growth of the mycelium (clearly visible on plates containing 0.1 M glycerol) for all strains, and development of perithecia (clearly visible as small black dots on plates containing 0.05 M glycerol) in all strains except for the PaGut2Δ mutant.	2024-04-29	Gautier, Valérie;Nguyen, Tinh-Suong;Silar, Philippe		Zenodo	biologists	Gautier, Valérie;Nguyen, Tinh-Suong;Silar, Philippe			
F34966167B0DFFBAFF45FA82FDC66D1A.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://zenodo.org/record/11093398/files/figure.png	https://doi.org/10.5281/zenodo.11093398	APPENDIX 3. — Growth of Escherichia coli T.Escherich on M0 medium supplemented with glycerol. Escherichia coli DH5α was inoculated at position indicated by the blue dots (except at the one on the center where Podospora anserina (Rabenh.) Niessl would be inoculated when the plates contain the fungus, e.g. see Fig. 7) on the indicated media and the plates were incubated for 20 days at 27°C under constant illumination at which point photographs were taken. In these conditions, the growth of the E. coli colonies is readily visible after one week of incubation and starts to mask the blue dots, except on M0 that does not permit the growth of E. coli.	APPENDIX 3. — Growth of Escherichia coli T.Escherich on M0 medium supplemented with glycerol. Escherichia coli DH5α was inoculated at position indicated by the blue dots (except at the one on the center where Podospora anserina (Rabenh.) Niessl would be inoculated when the plates contain the fungus, e.g. see Fig. 7) on the indicated media and the plates were incubated for 20 days at 27°C under constant illumination at which point photographs were taken. In these conditions, the growth of the E. coli colonies is readily visible after one week of incubation and starts to mask the blue dots, except on M0 that does not permit the growth of E. coli.	2024-04-29	Gautier, Valérie;Nguyen, Tinh-Suong;Silar, Philippe		Zenodo	biologists	Gautier, Valérie;Nguyen, Tinh-Suong;Silar, Philippe			
