taxonID	type	format	identifier	references	title	description	created	creator	contributor	publisher	audience	source	license	rightsHolder	datasetID
40124450514C545392F5E007805DB55A.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://binary.pensoft.net/fig/1704967	https://doi.org/10.3897/imafungus.17.189848.figure2	Figure 2. Macro- and microscopic characters of Semicentenialea rex sp. nov. Nuclei stained with propidium iodine (magenta) and cell walls and septa with calcofluor white (cyan), maximum intensity projection of a z-stack in C – D. A Colony growth after eight weeks (petri dish diameter = 90 mm; HU 4064, type). B Hypha with clamp connections (arrows) (JH 144). C Hyphae with clamp connections (arrows), one with a nucleus in the forming clamp (HU 4064, type). D Overview of the hyphae with clamp connections (arrows) and nuclei. Different developmental stages of three types of swellings: basidium-like cells (hereinafter BLC), probasidium / teliospore-like cells (hereinafter TLC), and intercalary swelling can also be seen (HU 4064, type), for detailed description of these structures see Figs 3, 4. Rotating 3 D rendering of D as Suppl. material 2: movie S 1. Scale bars 10 μm.	Figure 2. Macro- and microscopic characters of Semicentenialea rex sp. nov. Nuclei stained with propidium iodine (magenta) and cell walls and septa with calcofluor white (cyan), maximum intensity projection of a z-stack in C – D. A Colony growth after eight weeks (petri dish diameter = 90 mm; HU 4064, type). B Hypha with clamp connections (arrows) (JH 144). C Hyphae with clamp connections (arrows), one with a nucleus in the forming clamp (HU 4064, type). D Overview of the hyphae with clamp connections (arrows) and nuclei. Different developmental stages of three types of swellings: basidium-like cells (hereinafter BLC), probasidium / teliospore-like cells (hereinafter TLC), and intercalary swelling can also be seen (HU 4064, type), for detailed description of these structures see Figs 3, 4. Rotating 3 D rendering of D as Suppl. material 2: movie S 1. Scale bars 10 μm.	2026-07-01	Nogerius, Veera Tuovinen;Sánchez-García, Marisol;Kluting, Kerri;Heinonsalo, Jussi;Ryberg, Martin;Toome, Merje;Haridas, Sajeet;Mondo, Stephen;LaButti, Kurt;Nolan, Matt;Lipzen, Anna;Koriabine, Maxim;Bauer, Diane;Barry, Kerrie;Grigoriev, Igor V.;Aime, M. Catherine;Rosling, Anna		Zenodo	biologists	Nogerius, Veera Tuovinen;Sánchez-García, Marisol;Kluting, Kerri;Heinonsalo, Jussi;Ryberg, Martin;Toome, Merje;Haridas, Sajeet;Mondo, Stephen;LaButti, Kurt;Nolan, Matt;Lipzen, Anna;Koriabine, Maxim;Bauer, Diane;Barry, Kerrie;Grigoriev, Igor V.;Aime, M. Catherine;Rosling, Anna			
40124450514C545392F5E007805DB55A.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://binary.pensoft.net/fig/1704968	https://doi.org/10.3897/imafungus.17.189848.figure3	Figure 3. Variation in the morphology of the basidium-like cells (BLC) in Semicentenialea rex sp. nov. G – L 3 D volume renderings of z-stacks, nuclei stained with propidium iodine (magenta) and cell walls and septa with calcofluor white (cyan). A – F BLCs with basal clamp connections (arrows). C A lateral cell arising from the BLC stalk (arrowhead). G BLCs with lateral cells with one nucleus (arrows), one BLC with a ring of strong calcofluor white staining in the obovoid cell and one nucleus in each department. Clamp connection forming (arrowhead), and yeast cells budding from hyphae indicated with strong calcofluor white staining at the base (asterisk). H BLC formed from a thick-walled probasidial-like swelling (TLC) (arrowhead), forming a second BLC with two nuclei in the obovoid cell and one in the lateral cell (arrow). Rotating 3 D rendering of H as Suppl. material 4: movie S 3. I Two globose BLCs in a string. J Lateral cell of a BLC anastomosing to the generative hypha (arrow), three nuclei in the obovoid cell. K BLC with a basal clamp connection (arrow). L Possible early stage of a BLC with four nuclei. Isolate HU 4107 (A, C – F); JH 144 (B), HU 4064, type (G – L). Scale bars 10 μm (A – G); 4 μm (H – I, K); 5 μm (J); 3 μm (L).	Figure 3. Variation in the morphology of the basidium-like cells (BLC) in Semicentenialea rex sp. nov. G – L 3 D volume renderings of z-stacks, nuclei stained with propidium iodine (magenta) and cell walls and septa with calcofluor white (cyan). A – F BLCs with basal clamp connections (arrows). C A lateral cell arising from the BLC stalk (arrowhead). G BLCs with lateral cells with one nucleus (arrows), one BLC with a ring of strong calcofluor white staining in the obovoid cell and one nucleus in each department. Clamp connection forming (arrowhead), and yeast cells budding from hyphae indicated with strong calcofluor white staining at the base (asterisk). H BLC formed from a thick-walled probasidial-like swelling (TLC) (arrowhead), forming a second BLC with two nuclei in the obovoid cell and one in the lateral cell (arrow). Rotating 3 D rendering of H as Suppl. material 4: movie S 3. I Two globose BLCs in a string. J Lateral cell of a BLC anastomosing to the generative hypha (arrow), three nuclei in the obovoid cell. K BLC with a basal clamp connection (arrow). L Possible early stage of a BLC with four nuclei. Isolate HU 4107 (A, C – F); JH 144 (B), HU 4064, type (G – L). Scale bars 10 μm (A – G); 4 μm (H – I, K); 5 μm (J); 3 μm (L).	2026-07-01	Nogerius, Veera Tuovinen;Sánchez-García, Marisol;Kluting, Kerri;Heinonsalo, Jussi;Ryberg, Martin;Toome, Merje;Haridas, Sajeet;Mondo, Stephen;LaButti, Kurt;Nolan, Matt;Lipzen, Anna;Koriabine, Maxim;Bauer, Diane;Barry, Kerrie;Grigoriev, Igor V.;Aime, M. Catherine;Rosling, Anna		Zenodo	biologists	Nogerius, Veera Tuovinen;Sánchez-García, Marisol;Kluting, Kerri;Heinonsalo, Jussi;Ryberg, Martin;Toome, Merje;Haridas, Sajeet;Mondo, Stephen;LaButti, Kurt;Nolan, Matt;Lipzen, Anna;Koriabine, Maxim;Bauer, Diane;Barry, Kerrie;Grigoriev, Igor V.;Aime, M. Catherine;Rosling, Anna			
40124450514C545392F5E007805DB55A.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://binary.pensoft.net/fig/1704969	https://doi.org/10.3897/imafungus.17.189848.figure4	Figure 4. Intercalary and probasidium / teliospore-like (TLC) swellings and promycelium-like hyphae in Semicentenialea rex sp. nov. Nuclei stained with propidium iodine (magenta) and cell walls and septa with calcofluor white (cyan). 3 D volume rendering of z-stacks in B – C, G – I, K – M. Maximum intensity projection in Q. A An intercalary swelling separated by two septa, with a potential cell budding of close to one of them (arrow). Short terminal cell at the end of the same hypha (arrowhead). B A budding cell close to an intercalary swelling with one nucleus (arrow). C An intercalary swelling separated by septa on both sides. A budding cell (arrow) from the adjacent cell to the intercalary swelling. D Intercalary swelling with clamps forming on both sides (arrowheads). E A thick-walled, sessile probasidium / teliospore-like swelling. F – G Detached TLC with a spore-like cell forming inside (asterisk), with two nuclei in G. H TLC with a spore-like cell with three nuclei. I TLC attached to the generative hypha with budding cell with one nucleus, and two nuclei inside the spore-like cell. J One optical section of I showing the binucleate spore-like cell inside the TLC. K Binucleate spore-like cells released from TLCs. L TLCs in different developmental stages, with the spore-like cells more visible in some cells (asterisk). M Possibly early developmental stages of TLCs with two spots staining strongly with calcofluor white, hypothetical initial stages of the cell-wall of the spore-like cell. Rotating 3 D rendering of M as Suppl. material 6: movie S 5. N Detached TLC starting to germinate (arrow) and a released spore (arrowhead) O TLC germinating and forming a thick promycelium-like hypha, with possible conidial cells at one end (arrow). P Promycelium and thin, septate hypha (arrow). Q Terminal TLC and dikaryotic hyphae. R – S Knobbly promycelium with possible small yeasts budding of (arrow). T Promycelium with clamp connection (c) and budding cell (arrow). A single cell germinating (arrowhead). U Yeast cell (arrowhead) budding of from a knobbly promycelium. Isolate HU 4064, type (A – D, G – L, T); HU 4107 (E – F, N – P); HU 4147 (M, Q); JH 144 (R – S, U). Scale bars: 10 μm (A, C – F, K, M – P, Q – U); 5 μm (B, G, J, L), 4 μm (H), 3 μm (I).	Figure 4. Intercalary and probasidium / teliospore-like (TLC) swellings and promycelium-like hyphae in Semicentenialea rex sp. nov. Nuclei stained with propidium iodine (magenta) and cell walls and septa with calcofluor white (cyan). 3 D volume rendering of z-stacks in B – C, G – I, K – M. Maximum intensity projection in Q. A An intercalary swelling separated by two septa, with a potential cell budding of close to one of them (arrow). Short terminal cell at the end of the same hypha (arrowhead). B A budding cell close to an intercalary swelling with one nucleus (arrow). C An intercalary swelling separated by septa on both sides. A budding cell (arrow) from the adjacent cell to the intercalary swelling. D Intercalary swelling with clamps forming on both sides (arrowheads). E A thick-walled, sessile probasidium / teliospore-like swelling. F – G Detached TLC with a spore-like cell forming inside (asterisk), with two nuclei in G. H TLC with a spore-like cell with three nuclei. I TLC attached to the generative hypha with budding cell with one nucleus, and two nuclei inside the spore-like cell. J One optical section of I showing the binucleate spore-like cell inside the TLC. K Binucleate spore-like cells released from TLCs. L TLCs in different developmental stages, with the spore-like cells more visible in some cells (asterisk). M Possibly early developmental stages of TLCs with two spots staining strongly with calcofluor white, hypothetical initial stages of the cell-wall of the spore-like cell. Rotating 3 D rendering of M as Suppl. material 6: movie S 5. N Detached TLC starting to germinate (arrow) and a released spore (arrowhead) O TLC germinating and forming a thick promycelium-like hypha, with possible conidial cells at one end (arrow). P Promycelium and thin, septate hypha (arrow). Q Terminal TLC and dikaryotic hyphae. R – S Knobbly promycelium with possible small yeasts budding of (arrow). T Promycelium with clamp connection (c) and budding cell (arrow). A single cell germinating (arrowhead). U Yeast cell (arrowhead) budding of from a knobbly promycelium. Isolate HU 4064, type (A – D, G – L, T); HU 4107 (E – F, N – P); HU 4147 (M, Q); JH 144 (R – S, U). Scale bars: 10 μm (A, C – F, K, M – P, Q – U); 5 μm (B, G, J, L), 4 μm (H), 3 μm (I).	2026-07-01	Nogerius, Veera Tuovinen;Sánchez-García, Marisol;Kluting, Kerri;Heinonsalo, Jussi;Ryberg, Martin;Toome, Merje;Haridas, Sajeet;Mondo, Stephen;LaButti, Kurt;Nolan, Matt;Lipzen, Anna;Koriabine, Maxim;Bauer, Diane;Barry, Kerrie;Grigoriev, Igor V.;Aime, M. Catherine;Rosling, Anna		Zenodo	biologists	Nogerius, Veera Tuovinen;Sánchez-García, Marisol;Kluting, Kerri;Heinonsalo, Jussi;Ryberg, Martin;Toome, Merje;Haridas, Sajeet;Mondo, Stephen;LaButti, Kurt;Nolan, Matt;Lipzen, Anna;Koriabine, Maxim;Bauer, Diane;Barry, Kerrie;Grigoriev, Igor V.;Aime, M. Catherine;Rosling, Anna			
