taxonID	type	format	identifier	references	title	description	created	creator	contributor	publisher	audience	source	license	rightsHolder	datasetID
2C7FBF6B6C3A59A3B13A94EF277788FF.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://binary.pensoft.net/fig/1564529	https://doi.org/10.3897/subtbiol.55.177524.figure3	Figure 3. Morphological and size variations in Ephydatia fluviatilis from Sabak-Ha Cenote. A typically discoidal form, sponges in shaded areas at 8 m depth present a pink coloration and soft texture B growth form adapting to substrata instead of the typical discoidal form at 6 m depth C sponge CNPGG - 2670 in lighted areas present yellow to green color and a slightly harder texture, collected at 4 m depth D sponge CNPGG - 2671 collected at 11 m depth. Scale bars: 2 cm (A); 1 cm (B); 2 cm (C); 4 cm (D).	Figure 3. Morphological and size variations in Ephydatia fluviatilis from Sabak-Ha Cenote. A typically discoidal form, sponges in shaded areas at 8 m depth present a pink coloration and soft texture B growth form adapting to substrata instead of the typical discoidal form at 6 m depth C sponge CNPGG - 2670 in lighted areas present yellow to green color and a slightly harder texture, collected at 4 m depth D sponge CNPGG - 2671 collected at 11 m depth. Scale bars: 2 cm (A); 1 cm (B); 2 cm (C); 4 cm (D).	2026-03-18	Hernández-Solis, Pablo Alberto;Gómez-Bretón, Víctor Eduardo;de la Cruz-Fernández, Dalia Angélica;Simões, Nuno		Zenodo	biologists	Hernández-Solis, Pablo Alberto;Gómez-Bretón, Víctor Eduardo;de la Cruz-Fernández, Dalia Angélica;Simões, Nuno			
2C7FBF6B6C3A59A3B13A94EF277788FF.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://binary.pensoft.net/fig/1564531	https://doi.org/10.3897/subtbiol.55.177524.figure5	Figure 5. Morphological and size variations in Ephydatia fluviatilis from Suhem Cenote. Specimens collected at depths of 4 m (A), 4.7 m (B), and 5.3 m (C) C this photography belongs to the collected sponge CNPGG - 2673. Scale bars: 1 cm (A, B); 4 cm (C).	Figure 5. Morphological and size variations in Ephydatia fluviatilis from Suhem Cenote. Specimens collected at depths of 4 m (A), 4.7 m (B), and 5.3 m (C) C this photography belongs to the collected sponge CNPGG - 2673. Scale bars: 1 cm (A, B); 4 cm (C).	2026-03-18	Hernández-Solis, Pablo Alberto;Gómez-Bretón, Víctor Eduardo;de la Cruz-Fernández, Dalia Angélica;Simões, Nuno		Zenodo	biologists	Hernández-Solis, Pablo Alberto;Gómez-Bretón, Víctor Eduardo;de la Cruz-Fernández, Dalia Angélica;Simões, Nuno			
2C7FBF6B6C3A59A3B13A94EF277788FF.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://binary.pensoft.net/fig/1564532	https://doi.org/10.3897/subtbiol.55.177524.figure6	Figure 6. Gemmule, spicules and skeletal architecture of Ephydatia fluviatilis from Sabak-Ha and Suhem Cenotes. A gemmule with visible birotules (Gml) B smooth oxeas C spiny oxeas exclusively from Sabak-Ha Cenote sampled sponges D skeletal network tracts (Tr) composed of oxeas (Ox), spongin (Sp) and alveolate cavities (Ac) by LM E spines (Sp) and microspines (Msp) of the birotules shaft by SEM F birotules gemmuloscleres by SEM G detail of a rotule (top view) by SEM A taken from specimen CNPGG - 2673 B – E, G taken from the specimen CNPGG - 2670 F taken from specimens CNPGG - 2670 and CNPGG - 2671. Scale bars: 50 μm (A, B); 20 μm (C) 100 μm (D); 2 μm (E); 10 μm (F, G).	Figure 6. Gemmule, spicules and skeletal architecture of Ephydatia fluviatilis from Sabak-Ha and Suhem Cenotes. A gemmule with visible birotules (Gml) B smooth oxeas C spiny oxeas exclusively from Sabak-Ha Cenote sampled sponges D skeletal network tracts (Tr) composed of oxeas (Ox), spongin (Sp) and alveolate cavities (Ac) by LM E spines (Sp) and microspines (Msp) of the birotules shaft by SEM F birotules gemmuloscleres by SEM G detail of a rotule (top view) by SEM A taken from specimen CNPGG - 2673 B – E, G taken from the specimen CNPGG - 2670 F taken from specimens CNPGG - 2670 and CNPGG - 2671. Scale bars: 50 μm (A, B); 20 μm (C) 100 μm (D); 2 μm (E); 10 μm (F, G).	2026-03-18	Hernández-Solis, Pablo Alberto;Gómez-Bretón, Víctor Eduardo;de la Cruz-Fernández, Dalia Angélica;Simões, Nuno		Zenodo	biologists	Hernández-Solis, Pablo Alberto;Gómez-Bretón, Víctor Eduardo;de la Cruz-Fernández, Dalia Angélica;Simões, Nuno			
2C7FBF6B6C3A59A3B13A94EF277788FF.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://binary.pensoft.net/fig/1564533	https://doi.org/10.3897/subtbiol.55.177524.figure7	Figure 7. Comparison of birotules from sponges collected in Sabak-Ha Cenote (A, B) and Suhem Cenote (C, D). Birotule A corresponds to specimen CNPGG - 2670 (Fig. 3 C), and birotule B to specimen CNPGG - 2671 (Fig. 3 D). Birotule C corresponds to specimen CNPGG - 2673 (Fig. 5 C), while birotule D corresponds to a sponge from Suhem not deposited in the CNPGG collection (Fig. 5 A). Scale bars: 10 μm.	Figure 7. Comparison of birotules from sponges collected in Sabak-Ha Cenote (A, B) and Suhem Cenote (C, D). Birotule A corresponds to specimen CNPGG - 2670 (Fig. 3 C), and birotule B to specimen CNPGG - 2671 (Fig. 3 D). Birotule C corresponds to specimen CNPGG - 2673 (Fig. 5 C), while birotule D corresponds to a sponge from Suhem not deposited in the CNPGG collection (Fig. 5 A). Scale bars: 10 μm.	2026-03-18	Hernández-Solis, Pablo Alberto;Gómez-Bretón, Víctor Eduardo;de la Cruz-Fernández, Dalia Angélica;Simões, Nuno		Zenodo	biologists	Hernández-Solis, Pablo Alberto;Gómez-Bretón, Víctor Eduardo;de la Cruz-Fernández, Dalia Angélica;Simões, Nuno			
2C7FBF6B6C3A59A3B13A94EF277788FF.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://binary.pensoft.net/fig/1564534	https://doi.org/10.3897/subtbiol.55.177524.figure8	Figure 8. Tree topology of rDNA region (18 s-ITS 1-5.8 - ITS 2 - 28 s) based on comparisons of 813 bp from 21 freshwater sponge samples, obtained by Bayesian Inference (MrBayes) and Maximum Likelihood (PhyML). The number at each node represents the BI Posterior Probability, followed by ML Bootstrap Values. The GenBank sequence accession code was included after each species name. Trochospongilla PP 853641 were used as outgroups. Taxon names of sequences obtained in this study are indicated in green.	Figure 8. Tree topology of rDNA region (18 s-ITS 1-5.8 - ITS 2 - 28 s) based on comparisons of 813 bp from 21 freshwater sponge samples, obtained by Bayesian Inference (MrBayes) and Maximum Likelihood (PhyML). The number at each node represents the BI Posterior Probability, followed by ML Bootstrap Values. The GenBank sequence accession code was included after each species name. Trochospongilla PP 853641 were used as outgroups. Taxon names of sequences obtained in this study are indicated in green.	2026-03-18	Hernández-Solis, Pablo Alberto;Gómez-Bretón, Víctor Eduardo;de la Cruz-Fernández, Dalia Angélica;Simões, Nuno		Zenodo	biologists	Hernández-Solis, Pablo Alberto;Gómez-Bretón, Víctor Eduardo;de la Cruz-Fernández, Dalia Angélica;Simões, Nuno			
2C7FBF6B6C3A59A3B13A94EF277788FF.taxon	http://purl.org/dc/dcmitype/StillImage	image/png	https://binary.pensoft.net/fig/1564535	https://doi.org/10.3897/subtbiol.55.177524.figure9	Figure 9. Distribution of six Ephydatia species across North America. Records of E. fluviatilis were obtained from the Global Biodiversity Information Facility (GBIF 2025 a), with historical records in Mexico from Pátzcuaro and Xochimilco Lakes documented by Rioja (1942) and Penney and Racek (1968), respectively, and a recent record of Ephydatia sp. in Nazas River by Rodríguez-Ríos et al. (2024). Records of E. millsii and E. robusta were obtained from Potts (1888), with additional E. robusta records from Laubenfels (1932) and GBIF (2025 b). Records of E. muelleri were obtained from Smith (1918) and Ricciardi and Reiswig (1993), records of E. subtilis were from Weltner (1895) and the record of E. facunda was from Manconi and Pronzato (2005).	Figure 9. Distribution of six Ephydatia species across North America. Records of E. fluviatilis were obtained from the Global Biodiversity Information Facility (GBIF 2025 a), with historical records in Mexico from Pátzcuaro and Xochimilco Lakes documented by Rioja (1942) and Penney and Racek (1968), respectively, and a recent record of Ephydatia sp. in Nazas River by Rodríguez-Ríos et al. (2024). Records of E. millsii and E. robusta were obtained from Potts (1888), with additional E. robusta records from Laubenfels (1932) and GBIF (2025 b). Records of E. muelleri were obtained from Smith (1918) and Ricciardi and Reiswig (1993), records of E. subtilis were from Weltner (1895) and the record of E. facunda was from Manconi and Pronzato (2005).	2026-03-18	Hernández-Solis, Pablo Alberto;Gómez-Bretón, Víctor Eduardo;de la Cruz-Fernández, Dalia Angélica;Simões, Nuno		Zenodo	biologists	Hernández-Solis, Pablo Alberto;Gómez-Bretón, Víctor Eduardo;de la Cruz-Fernández, Dalia Angélica;Simões, Nuno			
