identifier	taxonID	type	CVterm	format	language	title	description	additionalInformationURL	UsageTerms	rights	Owner	contributor	creator	bibliographicCitation
F9F6D30F039F5630A60C2BF501D98F7B.text	F9F6D30F039F5630A60C2BF501D98F7B.taxon	http://purl.org/dc/dcmitype/Text	http://rs.tdwg.org/ontology/voc/SPMInfoItems#GeneralDescription	text/html	en	Ochrolechia guizhouensis Zheng & Meng 2025	<div><p>Ochrolechia guizhouensis Zheng &amp; Meng sp. nov.</p><p>Fig. 2</p><p>Remark.</p><p>Ochrolechia guizhouensis is characterised by apothecia with rugose to rosulate discs presenting a distinct floral morphology. Chemically, lichesterinic acid is present in the apothecia, but absent in the thallus.</p><p>Type.</p><p>China • Guizhou, Qiandongnan Prefecture, Leigongshan Nat. Res, on bark, 1698 m elev., 2023, B. Liu and Z. Yang, LGS 219 (KUN-L 96618, holotype) .</p><p>Etymology.</p><p>The species epithet refers to Guizhou Province, the type locality where the species was collected.</p><p>Holotype.</p><p>KUN-L 96618.</p><p>Description.</p><p>Thallus greyish-green, thick, rugose to verruculose, dull; prothallus indistinct, isidia and soredia absent.</p><p>Sexual morph. Apothecia frequent, scattered, sometimes crowded, sessile, round or irregular, 0.5–0.8 mm diam.; disc light yellowish-green or light brown, rugose to rosulate, epruinose; margins thick, smooth and taller than disc when young, becoming thin, verruculose and as tall as disc when older, exciple well developed even extending to the disc surface, concolorous with the thallus, shiny. Pycnidia are absent. Hymenium hyaline and colourless, 360–370 μm high, paraphyses are branched and anastomosing; epihymenium brown, 9–11 μm high; hypothecium pale brown (the colour of the epihymenium and hypothecium disappears in a 10 % KOH solution), 30–40 μm high; coccoid green alga, algae forming a continuous layer in the margin and in scattered clumps below the hypothecium. Asci clavate, (269) 272–284 (288) × (48) 49–60 (62) μm (n = 10). Ascospores hyaline, aseptate, broadly ellipsoid, (57) 60–78 (82) × (26) 28–32 (34) μm (n = 31).</p><p>Chemistry.</p><p>Thallus contains gyrophoric acid and lecanoric acid; ascomata contain gyrophoric acid, lecanoric acid and lichesterinic acid (TLC). Thallus cortex: K + pale yellow, C + red, KC + red; medulla: C –; apothecia cortex: K + pale yellow, C + red, KC + yellow; medulla: C –; disc: C + red; thallus UV -.</p><p>Material examined.</p><p>China • Guizhou Province, Qiandongnan Prefecture City, <a href="https://tb.plazi.org/GgServer/search?materialsCitation.longitude=108.19515&amp;materialsCitation.latitude=26.378677" title="Search Plazi for locations around (long 108.19515/lat 26.378677)">Leigongshan National Nature Reserve</a>, 26°22'43.24"N, 108°11'42.54"E, 1698 m elev., on bark, 27 October, 2023, Bo Liu and Ze Yang, LGS 219 (KUN-L 96618, holotype) .</p><p>Notes.</p><p>In the phylogenetic tree, O. guizhouensis (LGS 219) and O. akagiensis (Hara Kojiro: 0007) are closely related although with weak bootstrap support (&lt;70 %). The ITS sequence divergence between them is 6.35 % (33 / 520 bp). Morphologically, the two species differ distinctly: O. guizhouensis lacks isidia on the thallus and produces pale yellow, smaller apothecia (0.5–0.8 mm in diameter), whereas O. akagiensis bears isidia and develops pinkish, larger apothecia (0.8–1.5 mm in diameter). Chemically, the apothecia of O. guizhouensis contain lichesterinic acid, while the presence of this compound has not been reported in O. akagiensis (Park et al. 2019) .</p><p>Although Ochrolechia guizhouensis resembles O. trochophora in some morphological and chemical characteristics, the two species differ notably in several features. The apothecia of O. guizhouensis are smaller (0.5–0.8 mm diam.), whereas those of O. trochophora are larger (1–3 (4) mm diam.) (Brodo 1991). In addition, the apothecia of the latter lack lichesterinic acid. Phylogenetic analyses further indicate that this new species is distantly related the two O. trochophora vouchers (AFTOL-ID 880 and J. Vondrak 15442), supported by sequence divergences of 13.76 % (67 / 487 bp) in the ITS region and 4.97 % (35 / 704 bp) in the mtSSU region between O. guizhouensis (LGS 219) and O. trochophora (J. Vondrak 15442).</p><p>The difference with another similar species O. margarita, based on the identification key of Ren (2017), is that this species has a very thin thallus and its apothecia become subglobose when mature. Moreover, the algal layer forms a continuous layer below the hymenium, containing only gyrophoric acid and a trace of lecanoric acid.</p></div>	https://treatment.plazi.org/id/F9F6D30F039F5630A60C2BF501D98F7B	Public Domain	No known copyright restrictions apply. See Agosti, D., Egloff, W., 2009. Taxonomic information exchange and copyright: the Plazi approach. BMC Research Notes 2009, 2:53 for further explanation.		Pensoft via Plazi	Zheng, Weiwei;He, Linzhi;Bo, Heyun;Jeewon, Rajesh;Jayawardena, Ruvishika S.;Wang, Yuxian;Wang, Jie;Fu, Shaobin;Meng, Qingfeng	Zheng, Weiwei, He, Linzhi, Bo, Heyun, Jeewon, Rajesh, Jayawardena, Ruvishika S., Wang, Yuxian, Wang, Jie, Fu, Shaobin, Meng, Qingfeng (2025): Three new species of Ochrolechia (Ochrolechiaceae, Pertusariales) from Guizhou Province, China. MycoKeys 126: 19-40, DOI: 10.3897/mycokeys.126.168652
E2CA1B0C178E59469C23494D7358E7C8.text	E2CA1B0C178E59469C23494D7358E7C8.taxon	http://purl.org/dc/dcmitype/Text	http://rs.tdwg.org/ontology/voc/SPMInfoItems#GeneralDescription	text/html	en	Ochrolechia kuankuoshuiensis Zheng & Meng 2025	<div><p>Ochrolechia kuankuoshuiensis Zheng &amp; Meng sp. nov.</p><p>Fig. 3</p><p>Remark.</p><p>Distinctive features of O. kuankuoshuiensis are the large ascospores ((90) 97–116 (123) × (30) 33–35 (36) μm) and chemistry, which is limited to gyrophoric acid and lecanoric acid.</p><p>Type.</p><p>China • Guizhou, Zunyi City, Kuankuoshui Nat. Res, on bark, 1529 m elev., 2023, WW. Zheng and B. Liu, LGS 219 (KUN-L 96619, holotype) .</p><p>Etymology.</p><p>The species epithet refers to Kuankuoshui, the locality where the type species was collected.</p><p>Holotype.</p><p>KUN-L 96619.</p><p>Description.</p><p>Thallus greyish-white, thick, scaly, dull, verruculose; prothallus indistinct; isidia and soredia absent.</p><p>Sexual morph. Apothecia frequent, mostly scattered, sometimes crowded, sessile, irregularly rounded or irregularly florid, 0.5–2.5 mm diam.; disc light yellow, epruinose, rough to rugose when young, with deep rugose when older, margins thick, concolorous with the thallus, shiny, verrucose and higher than disc when young, warts developing until they intersect with the folds of the disc at maturity. Pycnidia are absent. Hymenium hyaline, 410–483 μm high; paraphyses are branched, densely aggregated; epihymenium black brown (the colour disappears in a 10 % KOH solution), 103–125 μm high; hypothecium 50–75 μm high; coccoid green alga, algae forming a continuous layer in the margin and below the hypothecium; asci clavate, (280) 285–309 (312) × (66) 67–74 (75) μm (n = 10), 8 - spored. Ascospores (90) 97–116 (123) × (30) 33–35 (36) μm (n = 33), aseptate, hyaline, broadly ellipsoid.</p><p>Chemistry.</p><p>Thallus and ascomata contain grophoric acid and lecanoric acid (TLC). Thallus cortex: K –, C + red, KC + red; medulla: C –; apothecia cortex: K + yellow, C + red, KC + red; medulla: C –; disc: C + red; thallus UV -.</p><p>Material examined.</p><p>China • Guizhou Province, Zunyi City, <a href="https://tb.plazi.org/GgServer/search?materialsCitation.longitude=107.1546&amp;materialsCitation.latitude=28.237364" title="Search Plazi for locations around (long 107.1546/lat 28.237364)">Kuankuoshui National Nature Reserve</a>, 28°14'14.51"N, 107°9'16.55"E, 1529 m elev., on bark, 17 November, 2023, Weiwei Zheng and Bo Liu, KKS 83 (KUN-L 96619, holotype) ; • Qiandongnan Prefecture City, <a href="https://tb.plazi.org/GgServer/search?materialsCitation.longitude=108.20309&amp;materialsCitation.latitude=26.385202" title="Search Plazi for locations around (long 108.20309/lat 26.385202)">Leigongshan National Nature Reserve</a>, 26°23'6.73"N, 108°12'11.11"E, 2054 m elev., on bark, 17 October, 2023, Shaobin Fu and Ze Yang, LGS 40 (KUN-L 96620) .</p><p>Notes.</p><p>Phylogenetic analysis indicates that O. kuankuoshuiensis and O. parellula are closely related. However, there is a 2.62 % (13 / 496 bp) difference in the ITS sequence between strain KKS 83 ( O. kuankuoshuiensis) and strain KoLRI No. 018698 ( O. parellula). Additionally, O. parellula is a saxicolous species characterised by a smooth apothecial margin and smaller spores (40–52.5 × 20–22.5 μm) (Park et al. 2019).</p><p>Judging by the description of O. trochophora in Brodo (1991), O. kuankuoshuiensis is morphologically and chemically similar to this species. However, the latter has smaller spores (35–71 × (20) 23–35 (– 40) μm (Brodo 1991). Sequence comparison between the two species shows an ITS difference of 12.58 % (62 / 493 bp) and an mtSSU difference of 2.99 % (21 / 703 bp) between O. kuankuoshuiensis (KKS 83) and O. trochophora (J. Vondrak 15442). Another morphologically similar species, O. longispora, is characterised by a thin thallus, smooth to slightly verrucose apothecial margins and the presence of lichesterinic and protolichesterinic acid (Ren 2017). The ITS sequence difference between O. kuankuoshuiensis (KKS 83) and O. longispora (LGS 231-2) is 3.73 % (19 / 509 bp).</p></div>	https://treatment.plazi.org/id/E2CA1B0C178E59469C23494D7358E7C8	Public Domain	No known copyright restrictions apply. See Agosti, D., Egloff, W., 2009. Taxonomic information exchange and copyright: the Plazi approach. BMC Research Notes 2009, 2:53 for further explanation.		Pensoft via Plazi	Zheng, Weiwei;He, Linzhi;Bo, Heyun;Jeewon, Rajesh;Jayawardena, Ruvishika S.;Wang, Yuxian;Wang, Jie;Fu, Shaobin;Meng, Qingfeng	Zheng, Weiwei, He, Linzhi, Bo, Heyun, Jeewon, Rajesh, Jayawardena, Ruvishika S., Wang, Yuxian, Wang, Jie, Fu, Shaobin, Meng, Qingfeng (2025): Three new species of Ochrolechia (Ochrolechiaceae, Pertusariales) from Guizhou Province, China. MycoKeys 126: 19-40, DOI: 10.3897/mycokeys.126.168652
BCB6765CA6875E9B83BB7E81BD969340.text	BCB6765CA6875E9B83BB7E81BD969340.taxon	http://purl.org/dc/dcmitype/Text	http://rs.tdwg.org/ontology/voc/SPMInfoItems#GeneralDescription	text/html	en	Ochrolechia leigongshanensis Zheng & Fu 2025	<div><p>Ochrolechia leigongshanensis Zheng &amp; Fu sp. nov.</p><p>Fig. 4</p><p>Remark.</p><p>Ochrolechia leigongshanensis is distinguished by the presence of soralia and the thallus contains gyrophoric acid, lecanoric acid and atranorin. In most apothecia, a ring of smooth, salmon-pink tissue is visible along the inner edge of the apothecial margin.</p><p>Type.</p><p>China • Guizhou, Qiandongnan Prefecture, Leigongshan Nat. Res, on bark, 1666 m elev., 2023, B. Liu and Z. Yang, LGS 186 (KUN-L 96621, holotype) .</p><p>Etymology.</p><p>The new species has been named after Leigongshan, the locality where it was collected.</p><p>Holotype.</p><p>KUN-L 96621</p><p>Description.</p><p>Thallus greyish-white, thin, verruculose, dull; prothallus indistinct; isidia absent, with a few white soralia.</p><p>Sexual morph. Apothecia frequent, scattered or crowded, sessile, round, 1.9–2.7 mm diam.; disc plane, pale pink, epruinose to lightly pruinose, margins thick, clearly verrucose, higher than disc, shiny, concolorous with the thallus, a ring of smooth, salmon-pink tissue is distributed along the inner edge of the apothecial margin in most apothecia. Pycnidia are absent. Hymenium hyaline and colourless, 168–180 μm high; paraphyses are branched, densely aggregated; epihymenium dark brown (the colour disappears in a 10 % KOH solution), 10–13 μm high; hypothecium inconspicuous; coccoid green alga, algae forming a continuous layer in the margin and in scattered clumps below the hypothecium. Asci narrowly clavate, 4–8 - spored, 208–230 × 25–33 μm (n = 10). Ascospores hyaline, broadly ellipsoid, aseptate, (62) 64–69 (70) × (27) 28–33 (34) μm (n = 30).</p><p>Chemistry.</p><p>Thallus contains gyrophoric acid and lecanoric acid and atranorin; ascomata contain gyrophoric acid, lecanoric acid (TLC). Thallus cortex: K + pale yellow, C + red, KC + red; medulla: C –; apothecia cortex: K + yellow-green, C + red, KC + red to yellow-green; medulla: C –; disc: C + red; thallus UV –.</p><p>Material examined.</p><p>China • Guizhou Province, Qiandongnan Prefecture, <a href="https://tb.plazi.org/GgServer/search?materialsCitation.longitude=108.195145&amp;materialsCitation.latitude=26.37859" title="Search Plazi for locations around (long 108.195145/lat 26.37859)">Leigongshan National Nature Reserve</a>, 26°22'42.92"N, 108°11'42.51"E, 1666 m elev., on bark, 27 October, 2023, Bo Liu and Ze Yang, LGS 186 (KUN-L 96621, holotype) .</p><p>Notes.</p><p>Phylogenetic analysis places this species in a distinct, but weakly supported clade, sister to O. akagiensis and O. subrosella . Nucleotide comparison shows that O. leigongshanensis (LGS 186) differs from O. akagiensis (Hara Kojiro: 0007) by 8.07 % (39 / 483 bp) in the ITS region and from O. subrosella (LGS 176) by 8.49 % (41 / 483 bp) in the ITS sequence and 1.45 % (10 / 688 bp) in the mtSSU sequence. Moreover, both O. subrosella and O. akagiensis lack soralia and atranorin (Ren 2017) and a ring of salmon-pink tissue is absent from the inner edge of the apothecial margin. O. akagiensis produces isidia (Park et al. 2019).</p><p>According to the description of O. trochophora var. trochophora in the identification key by Ren (2017), O. leigongshanensis is similar to this taxon, but the latter lacks atranorin, without soredia and the excipular ring of salmon disc-like tissue is not visible (Brodo 1991). Judging by the description of O. montana in Brodo (1991), O. leigongshanensis is also similar to this taxon, but O. montana lacks soredia and has smaller apothecia (0.7–1.8 mm in diameter). Chemically, it produces gyrophoric acid and a trace of lecanoric acid as major substances, sometimes along with trace of an unidentified minor compound.</p></div>	https://treatment.plazi.org/id/BCB6765CA6875E9B83BB7E81BD969340	Public Domain	No known copyright restrictions apply. See Agosti, D., Egloff, W., 2009. Taxonomic information exchange and copyright: the Plazi approach. BMC Research Notes 2009, 2:53 for further explanation.		Pensoft via Plazi	Zheng, Weiwei;He, Linzhi;Bo, Heyun;Jeewon, Rajesh;Jayawardena, Ruvishika S.;Wang, Yuxian;Wang, Jie;Fu, Shaobin;Meng, Qingfeng	Zheng, Weiwei, He, Linzhi, Bo, Heyun, Jeewon, Rajesh, Jayawardena, Ruvishika S., Wang, Yuxian, Wang, Jie, Fu, Shaobin, Meng, Qingfeng (2025): Three new species of Ochrolechia (Ochrolechiaceae, Pertusariales) from Guizhou Province, China. MycoKeys 126: 19-40, DOI: 10.3897/mycokeys.126.168652
AE5BF1BC411E566FB1210D8B7192DAA3.text	AE5BF1BC411E566FB1210D8B7192DAA3.taxon	http://purl.org/dc/dcmitype/Text	http://rs.tdwg.org/ontology/voc/SPMInfoItems#GeneralDescription	text/html	en	Ochrolechia longispora Brodo & Q. Ren	<div><p>Ochrolechia longispora Brodo &amp; Q. Ren</p><p>Fig. 5</p><p>Remark.</p><p>Lichenologist 49: 67–84 (2017); Type: China, Shaanxi, Mt. Taibai, Wengong Temple, on Abies, 3600 m elev., 2005, Q. Ren 1081 (SDNU – holotype) .</p><p>Description.</p><p>Thallus greyish-green, thin to thick, verruculose, dull; prothallus distinct, pale yellow, shiny; isidia and soredia absent.</p><p>Sexual morph. Apothecia frequent, scattered or crowded, sessile, round, 0.5–1.0 mm diam., disc plane, pale yellow or pinkish, epruinose; margins thick, concolorous with the thallus, higher than disc, smooth or with a few verrucae, shiny. Pycnidia are absent. Hymenium hyaline and colourless, 340–420 μm high, paraphyses are branched, densely aggregated; epihymenium pale brown, 40–50 μm high; hypothecium pale brown (the colour of the epihymenium and hypothecium disappears in a 10 % KOH solution), 45–50 μm high, algae forming a continuous layer in the margin and in scattered clumps below the hypothecium. Asci narrowly clavate, (230) 235–251 (255) × (29) 31–50 (n = 10) μm. Ascospores simple, hyaline, aseptate, ellipsoid to broadly ellipsoid, (78) 82–89 (91) × (32) 35–43 (45) μm (n = 30).</p><p>Chemistry.</p><p>Thallus and ascomata contain gyrophoric acid, lecanoric acid, lichesterinic acid and traces of protolichesterinic acid (TLC). Thallus cortex: K + pale yellow, C + red, KC + red; medulla: C –; apothecia cortex: K + pale yellow, C + red, KC + red to yellow-green; medulla: C –; disc: C + red; thallus UV –.</p><p>Material examined.</p><p>China • Guizhou Province, Qiandongnan Prefecture, <a href="https://tb.plazi.org/GgServer/search?materialsCitation.longitude=108.19513&amp;materialsCitation.latitude=26.378725" title="Search Plazi for locations around (long 108.19513/lat 26.378725)">Leigongshan National Nature Reserve</a>, 26°22'43.41"N, 108°11'42.47"E, 1660 m elev., on bark, 27 October, 2023, Bo Liu and Ze Yang, LGS 213 -2 (KUN-L 96622) ; • 26°22'43.17"N, 108°11'42.62"E, 1682 m elev., on bark, 27 October, 2023, Bo Liu and Ze Yang, LGS 205 (KUN-L 96623) .</p><p>Notes.</p><p>This study provides the first DNA sequences for Ochrolechia longispora . O. longispora is distinguished by its larger spores and the presence of four specific compounds. Phylogenetic analysis shows that O. longispora is closely related to O. kuankuoshuiensis; however, we confirm that they are separate species. Since the key differences have already been detailed in the previous section, we will not repeat the comparison here.</p><p>According to Ren (2017), O. longispora is characterised by a thin, rugose to verruculose thallus; thick, smooth or sparsely verrucose apothecial margins; ascospores measuring 80–90 (– 105) × 32–42 μm; and the presence of gyrophoric acid, protolichesterinic acid, lichesterinic acid and a trace of lecanoric acid. These characteristics show a high degree of concordance with those of our specimens.</p></div>	https://treatment.plazi.org/id/AE5BF1BC411E566FB1210D8B7192DAA3	Public Domain	No known copyright restrictions apply. See Agosti, D., Egloff, W., 2009. Taxonomic information exchange and copyright: the Plazi approach. BMC Research Notes 2009, 2:53 for further explanation.		Pensoft via Plazi	Zheng, Weiwei;He, Linzhi;Bo, Heyun;Jeewon, Rajesh;Jayawardena, Ruvishika S.;Wang, Yuxian;Wang, Jie;Fu, Shaobin;Meng, Qingfeng	Zheng, Weiwei, He, Linzhi, Bo, Heyun, Jeewon, Rajesh, Jayawardena, Ruvishika S., Wang, Yuxian, Wang, Jie, Fu, Shaobin, Meng, Qingfeng (2025): Three new species of Ochrolechia (Ochrolechiaceae, Pertusariales) from Guizhou Province, China. MycoKeys 126: 19-40, DOI: 10.3897/mycokeys.126.168652
12DF6B285BF95AC89E50A5CFD0018FCB.text	12DF6B285BF95AC89E50A5CFD0018FCB.taxon	http://purl.org/dc/dcmitype/Text	http://rs.tdwg.org/ontology/voc/SPMInfoItems#GeneralDescription	text/html	en	Ochrolechia subrosella Z. F. Jia & Z. T. Zhao	<div><p>Ochrolechia subrosella Z. F. Jia &amp; Z. T. Zhao</p><p>Fig. 6</p><p>Remark.</p><p>Mycosystema 24 (2): 162 (2005); type: China, Yunnan, Dali, Wei WY 014-1 (HMAS-L – holotype) .</p><p>Description.</p><p>Thallus greyish-brown, thick, cracked, verruculose to verrucose, dull; prothallus indistinct; isidia and soredia absent.</p><p>Sexual morph. Apothecia frequent, scattered, sessile and round, becoming remarkably large at maturity (2.5–4 mm in diameter) and covered with white pruina Disc pale pink, plane when young, rough to rugose at maturity, followed by a radial bulge; margins thick and smooth when young, becoming thin and verrucose when older, slightly lighter in colour than disc. Pycnidia are absent. Hymenium hyaline, 290–340 μm high; epihymenium brown (the colour partially or completely disappears in a 10 % KOH solution), 20–25 μm high; hypothecium 20–30 μm high; coccoid green alga, algae forming a continuous layer in the margin and below the hypothecium. Asci narrowly clavate, 8 - spored, (210) 218–243 (250) × (51) 56–67 (70) μm (n = 11). Ascospores hyaline, aseptate, ellipsoid to broadly ellipsoid, (50) 54–64 (67) × (22) 25–31 (33) μm (n = 30).</p><p>Chemistry.</p><p>Thallus and ascomata contain gyrophoric acid and lecanoric acid (TLC). Thallus cortex: K –, C + red, KC + red; medulla: C –; apothecia cortex: K + yellow-green, C + red, KC + red to yellow-green; medulla: C –; disc: C + red; thallus UV –.</p><p>Material examined.</p><p>China • Guizhou Province, Qiandongnan Prefecture City, <a href="https://tb.plazi.org/GgServer/search?materialsCitation.longitude=108.19534&amp;materialsCitation.latitude=26.378283" title="Search Plazi for locations around (long 108.19534/lat 26.378283)">Leigongshan National Nature Reserve</a>, 26°22'41.82"N, 108°11'43.23"E, 1670 m elev., on bark, 27 October, 2023, Bo Liu and Ze Yang, LGS- 176 (KUN-L 96624) .</p><p>Notes.</p><p>This study provides the first DNA sequences for Ochrolechia subrosella . Phylogenetic analysis shows that this species is closely related to O. akagiensis and O. leigongshanensis, but forms a distinct, weakly-supported clade. Sequence comparison reveals that O. subrosella (LGS 176) differs from O. akagiensis (Hara Kojiro: 0007) by 6.93 % (37 / 534 bp) in ITS and from O. leigongshanensis (LGS 186) by 8.49 % (41 / 483 bp) in ITS and 1.45 % (10 / 688 bp) in mtSSU.</p><p>Morphologically, this species is characterised by mature apothecia with expanded discs bearing striated protuberances and conspicuous radial ridges, consistent with the original description of O. subrosella (Jia and Zhao 2005) . Chemically, TLC confirmed the presence of gyrophoric acid and lecanoric acid in LGS 176, matching the secondary metabolite profile reported for O. subrosella .</p></div>	https://treatment.plazi.org/id/12DF6B285BF95AC89E50A5CFD0018FCB	Public Domain	No known copyright restrictions apply. See Agosti, D., Egloff, W., 2009. Taxonomic information exchange and copyright: the Plazi approach. BMC Research Notes 2009, 2:53 for further explanation.		Pensoft via Plazi	Zheng, Weiwei;He, Linzhi;Bo, Heyun;Jeewon, Rajesh;Jayawardena, Ruvishika S.;Wang, Yuxian;Wang, Jie;Fu, Shaobin;Meng, Qingfeng	Zheng, Weiwei, He, Linzhi, Bo, Heyun, Jeewon, Rajesh, Jayawardena, Ruvishika S., Wang, Yuxian, Wang, Jie, Fu, Shaobin, Meng, Qingfeng (2025): Three new species of Ochrolechia (Ochrolechiaceae, Pertusariales) from Guizhou Province, China. MycoKeys 126: 19-40, DOI: 10.3897/mycokeys.126.168652
8F464CDC0A9A5A00BEA2E9E6E6453064.text	8F464CDC0A9A5A00BEA2E9E6E6453064.taxon	http://purl.org/dc/dcmitype/Text	http://rs.tdwg.org/ontology/voc/SPMInfoItems#GeneralDescription	text/html	en	Ochrolechia trochophora var. trochophora (Vain.) Oshio	<div><p>Ochrolechia trochophora (Vain.) Oshio var. trochophora</p><p>Fig. 7</p><p>Remark.</p><p>J. Sci. Hiroshima Univ., Ser. B, Div. 2 (12): 145 (1968). – Pertusaria trochophora Vain., Bot. Mag. (Tokyo) 32: 155 (1918).</p><p>Type.</p><p>Japan • Prov. Kozuke, Mt. Akagi. Ad corticem arboris, Yasuda 53 (TUR-V–7255 – holotype, not seen; see Brodo (1991: 762); TI – isotype, not seen; see (Oshio 1968: 145)).</p><p>Description.</p><p>Thallus greyish-white to greyish-green, thin, verruculose, dull; isidia absent, prothallus indistinct, soredia greyish-white.</p><p>Sexual morph. Apothecia frequent, scattered, sessile, ellipsoid or round, 1.1–1.8 mm diam.; disc pale pink, plane to lightly rugose, epruinose, with a hazy white film; margin smooth or verruculose, higher than disc, dull, concolorous with the thallus. Pycnidia are absent. Hymenium hyaline and colourless, 380–392 μm high; epihymenium brown, 20–28 μm high; hypothecium greyish-brown (the colour of the epihymenium and hypothecium partially or completely disappears in a 10 % KOH solution), 10–17 μm high; coccoid green alga, algae absent or spotty in the margin and continuous below the hypothecium. Asci clavate, 8 - spored, (359) 363–372 (375) × (42) 43–49 (52) μm. Ascospores hyaline, aseptate, broadly ellipsoid, (50) 55–70 (75) × (22) 23–26 (28) μm (n = 30).</p><p>Chemistry.</p><p>Thallus contains gyrophoric acid, lecanoric acid and atranorin; ascomata contain gyrophoric acid and lecanoric acid. (TLC). soredia: K + yellow-green, C –, KC –; thallus cortex: K + yellow, C + red, KC + red to yellow-green; medulla: C –; apothecia cortex: K + yellow, C + red, KC + red to yellow-green; medulla: C –; disc: C + red; thallus UV –.</p><p>Material examined.</p><p>China • Guizhou Province, Zunyi City, <a href="https://tb.plazi.org/GgServer/search?materialsCitation.longitude=107.17341&amp;materialsCitation.latitude=28.20815" title="Search Plazi for locations around (long 107.17341/lat 28.20815)">Kuankoshui National Nature Reserve</a>, 28°12'29.34"N, 107°10'24.27"E, 1418 m elev., on bark, 18 November, 2023, Weiwei Zheng and Bo Liu, KKS 150-2 (KUN-L 96625) .</p><p>Notes.</p><p>Ochrolechia trochophora is a widely distributed species reported from several locations worldwide. It is primarily characterised by verruculose apothecial margins lacking or containing scattered algal cells. The key distinction between O. trochophora var. trochophora and O. trochophora var. pruinirosella lies in the fact that the latter has a pruinose apothecial disc and the vast majority of its specimens contain variolaric acid. In contrast, O. trochophora var. trochophora occasionally exhibits a hazy white film on the disc, but this is not pruina and very few specimens contain variolaric acid or atranorin (Brodo 1991). As reported by Kukwa (2009), European and Turkish specimens of O. trochophora var. trochophora possess a relatively thin thallus. Furthermore, the detection of atranorin in one Chinese specimen aligns with these findings. Collectively, this evidence supports the identification of specimen KKS 150-2 as O. trochophora var. trochophora .</p><p>The specimen also bears soredia, which is a relatively distinctive variation, as according to previous reports, no records of soredia have been documented in O. trochophora (whether the typical variety or other known varieties) (Brodo 1991; Kukwa 2009). This indicates that O. trochophora might be morphologically more variable than previously thought.</p><p>In Ochrolechia, many species (e. g. O. africana, O. antillarum, O. gowardii, O. trochophora) contain small crystalline structures in the apothecial medulla (Brodo 1991). In the species we describe, the brownish appearance of both the epihymenium and hypothecium in apothecial sections is more likely a structural colour rather than a pigment-based one. This conclusion is primarily based on the partial or complete fading of the brown colour when treated with 10 % KOH, likely due to the dissolution of abundant granular or crystalline substances in the tissues.</p><p>Traditionally, the classification of species within the genus Ochrolechia has primarily relied on a combined analysis of morphological characteristics and chemical compounds. Morphologically, key diagnostic features include thallus thickness, the presence or absence of isidia and soredia, apothecial morphology, hymenium height, spore size and the position of the algal layer. Chemically, species in this genus exhibit a remarkable diversity of secondary metabolites, which serve as critical taxonomic markers. These compounds mainly belong to the following classes: Orcinol depsides (e. g. gyrophoric acid, lecanoric acid and olivetoric acid), orcinol depsidones (e. g. variolaric acid and alectoronic acid), higher aliphatic acids (e. g. lichesterinic acids, protolichesterinic acids and murolic acids) and xanthones, which can induce yellow fluorescence in the thallus under long-wave ultraviolet light. Additionally, trace amounts of atranorin have been detected in some species (Brodo 1991; Kukwa 2009).</p><p>In our study, thin-layer chromatography revealed significant levels of the lichen secondary metabolite atranorin in both the newly-described species O. leigongshanensis and the known species O. trochophora var. trochophora . This finding contrasts with the previous understanding that atranorin exists only in trace amounts within the genus Ochrolechia . Notably, these two species share a key morphological characteristic, the presence of soredia. The combination of this chemical and morphological feature provides valuable new insights into the evolutionary relationships within Ochrolechia .</p><p>Soredia are rarely observed in Ochrolechia and atranorin is not usually a major metabolite in this genus. The discovery that both O. leigongshanensis and O. trochophora var. trochophora possess these two characteristics strongly suggests that they may belong to a distinct phylogenetic lineage previously unrecognised. The production of soredia, as an asexual reproductive structure, is linked to specific genotypes, while the substantial synthesis of atranorin indicates the activation of particular biochemical pathways. The stable co-existence of these two independent traits, both morphological and chemical, within a limited taxonomic group is unlikely to be coincidental and likely represents synapomorphies inherited from a common ancestor. Thus, we hypothesise that these two species may share a most recent common ancestor within Ochrolechia, potentially forming a monophyletic group.</p><p>With the rapid advancement of molecular techniques, phylogenetic analysis has become an indispensable tool in species identification and evolutionary studies. Molecular data provide objective genetic evidence that enables the effective differentiation of morphologically similar and cryptic species, clarifying taxonomic uncertainties, such as synonymy and facilitating the discovery of new taxa (Spatafora et al. 2006; Miadlikowska et al. 2014). Relying solely on phenotypic characteristics often fails to resolve taxonomic ambiguities, as these features are influenced by environmental factors and developmental stages. In contrast, genetic data offer greater stability. Therefore, this study adopts an integrative taxonomic approach, combining phylogenetic analysis, morphological examination and chemical profiling to provide a comprehensive and accurate classification of new Ochrolechia species.</p><p>The phylogenetic analysis in this study revealed that all newly-described species form a single clade (Fig. 1). Although bootstrap support for some branches within this clade was relatively low (&lt;70 %), we interpret this not to ambiguous taxon delimitation, but rather to the substantial genetic distances amongst these new species or it might be due to the insufficient sampling in this clade. As more sequences of Ochrolechia species are published in the future, the topology may become more stable. Such considerable interspecific genetic divergence may result in a higher number of ambiguous alignment sites, leading to reduced nodal support in phylogenetic reconstructions. This phenomenon strongly suggests that the present study may have only uncovered a fraction of the diversity within the genus Ochrolechia in Guizhou, China. Limitations in sampling scope and specimen numbers currently hinder a full assessment of the true morphological variation and geographical distribution ranges of these species, which may also contribute to the temporarily unresolved phylogenetic relationships.</p><p>It is important to note that, while molecular support could be further strengthened through additional gene loci or expanded sampling in the future, all new species described in this study exhibit unique combinations of morphological and chemical characteristics, enabling clear distinction from all known related species.</p><p>In conclusion, the distinct clade formed by the new species described in this study not only enhances our understanding of the phylogenetic framework of Ochrolechia, but also highlights significant genetic variation within the clade, suggesting that the region may harbour an underexplored diversity of Ochrolechia . Future studies should prioritise more extensive and systematic specimen collection from this region and neighbouring areas, combined with multi-locus genomic data, to thoroughly elucidate the speciation mechanisms and the broader diversity of this genus.</p></div>	https://treatment.plazi.org/id/8F464CDC0A9A5A00BEA2E9E6E6453064	Public Domain	No known copyright restrictions apply. See Agosti, D., Egloff, W., 2009. Taxonomic information exchange and copyright: the Plazi approach. BMC Research Notes 2009, 2:53 for further explanation.		Pensoft via Plazi	Zheng, Weiwei;He, Linzhi;Bo, Heyun;Jeewon, Rajesh;Jayawardena, Ruvishika S.;Wang, Yuxian;Wang, Jie;Fu, Shaobin;Meng, Qingfeng	Zheng, Weiwei, He, Linzhi, Bo, Heyun, Jeewon, Rajesh, Jayawardena, Ruvishika S., Wang, Yuxian, Wang, Jie, Fu, Shaobin, Meng, Qingfeng (2025): Three new species of Ochrolechia (Ochrolechiaceae, Pertusariales) from Guizhou Province, China. MycoKeys 126: 19-40, DOI: 10.3897/mycokeys.126.168652
