identifier	taxonID	type	CVterm	format	language	title	description	additionalInformationURL	UsageTerms	rights	Owner	contributor	creator	bibliographicCitation
827887CEFFCE7002B1BB10B2E6D7FEB2.text	827887CEFFCE7002B1BB10B2E6D7FEB2.taxon	http://purl.org/dc/dcmitype/Text	http://rs.tdwg.org/ontology/voc/SPMInfoItems#GeneralDescription	text/html	en	Colletotrichum guttulatum Norph. & G. Shiqi 2025	<div><p>Colletotrichum­guttulatum­ Norph. &amp; G. Shiqi sp. nov.</p><p>Index Fungorum mumber: IF903943, Figures 3</p><p>Etymology: refers to the guttulate nature of the conidia, “ bearing guttules ”</p><p>Holotype: HGUP 25-0045</p><p>Isolated from the symptomatic branch of Heptapleurum heptaphyllum (L.) Y.F. Deng ( Araliaceae). Sexual morph: Undetermined. Asexual morph: Conidiomata pycnidial, globose, dark brown, superficial on PDA, releasing conidia in a yellow mass, slimy. Conidiophores pale brown, smooth-walled, septate, unbranched, (13–)16–43(–44) × (3–)4– 5(–5.5) μm (mean ± SD = 24 ± 1 × 4 ± 0.5 μm). Conidiogenous cells hyaline to pale brown, smooth-walled, cylindrical to ampulliform, flask-shaped, (9.3–)10–19(–20) × (3.4–)4–5(–5.3) μm (mean ± SD = 15 ± 1 × 4 ± 0.3 μm), at collarette 1–2 μm (width). Setae brown to dark brown, smooth-walled to finely verruculose, 2–4 septate, (30–)50–70(–100) µm long, base cylindrical to conical, 4–6(–9) µm diam., tip acute to roundish. Conidia (14–)15–19(–21) × (5.5–)5.8–6(–7) μm (mean ± SD = 16 ± 1 × 6 ± 0.2 μm), n = 60, L/W ratio = 2.8, hyaline, aseptate, smooth-walled, ellipsoidal to cylindrical, one end rounded and one end acute or both ends rounded, guttulate, granular. Appressoria absent.</p><p>Culture characteristics: Colony on PDA reaching 7–8 cm diam after 7 d at room temperature (±25 °C), under light 12 hr/dark 12 hr, colonies rhizoid to filamentous, flat surface, with filiform margin, pale grey or grey-green from above and white to pale-gray reverse, producing grouped pycnidia with raised mycelium surrounded. Colonies on CMA reaching 7–8 cm diam after 7 d at room temperature (±25 °C), under light 12 hr/dark 12 hr, colonies rhizoid to filamentous, dense, flat or raised surface, with filiform margin, white from above and white to pale-yellow reverse, with producing grouped-pycnidia. Colonies on WA with sterilized sticks, reaching 5 cm diam after 7 d at room temperature (±25 °C), under light 12 hr/dark 12 hr, colonies rhizoid to filamentous, dense, flat surface, with filiform margin, dark green from above and reverse, with producing pycnidia on sticks and immersed pycnidia under media.</p><p>Material examined: CHINA, Guizhou Province, Tongren, Foding Mountain, on symptomatic branch of Heptapleurum heptaphyllum (L.) Y.F. Deng ( Araliaceae), 11 July 2024, Guo Shiqi, D29 (HGUP 25-0045, holotype), ex-type living culture GUCC 25-0046, GUCC 25-0061 ; ibid. on symptomatic branch of Heptapleurum Gaertn. ( Araliaceae), 11 July 2024, Gou Shiqi, FDS8 (HGUP 25-0046, dried culture), living culture GUCC 25-0055, GUCC 25-0062 .</p><p>Notes: Based on morphological characteristics and multi-locus phylogenetic analyses, Colletotrichum guttulatum is a distinct clade within the C. dracaenophilum species complex. Colletotrichum guttulatum forms a sister clade to C. coelogynes, C. crousii, C. parallelophorum, and other closely related taxa (Figure 1), with support of 82% maximum likelihood bootstrap values and 0.99 Bayesian posterior probabilities. Morphologically, C. guttulatum shares common characteristics with other species in the complex, including hyaline, smooth-walled, aseptate, straight, cylindrical conidia with rounded apices and bases. However, it is distinguishable from its closest relatives by subtle differences in conidial dimensions: (14–)15–19(–21) × (5.5–)5.8–6(–7) μm in C. guttulatum versus (15–)15.5–18(–20) × (4.5–)5–6(–6.5) μm in C. coelogynes, 12.5–18.5 × 6.5–8 μm in C. crousii, and (12.1–)13.8–16.8(–18.9) × (3.3–)4.4– 7.5(–7.9) µm in C. parallelophorum (Ma et al. 2018, Liu et al. 2022). The conidia of C. guttulatum are slightly longer and narrower in comparison.</p><p>Pairwise nucleotide comparisons of Colletotrichum dracaenophilum with C. coelogynes, C. crousii, and C. parallelophorum revealed consistent genetic differences across all loci. The observed differences include: ITS: 3% (17/541), 5% (27/533), and 4% (15/433) with 2, 7, and 3 gaps, respectively; act: 7.5% (21/277), 7% (17/255), no data for C. parallelophorum; 5, 3, and no gaps; gapdh: 16% (44/285), 16% (46/281), and 22% (57/259) with 6, 4, and 14 gaps; β-tubulin: 7% (37/534), 8% (57/704), and 7.3% (33/455) with 1, 8, and 1 gap; chs-1: 2% (6/300), 3.6% (11/300), no gaps; his3: 8.7% (34/393), 9% (35/392) with 2 and 3 gaps, respectively. Colletotrichum coelogynes and C. crousii do not contain the cal gene, whereas C. parallelophorum lacks the act, chs-1, his3, and cal genes. These molecular differences, particularly the high variation observed across multiple loci, provide strong evidence for the taxonomic separation of C. dracaenophilum from its relatives.</p><p>In addition to morphological and phylogenetic evidence, the pairwise homoplasy index (PHI) test showed no significant signs of recombination between C. guttulatum and its phylogenetically closest relatives, C. coelogynes, C. crousii, and C. parallelophorum (Φw = 0.9942; Figure 2), supporting its genetic distinctiveness. The combination of phylogenetic support, clear morphological differentiation, and the absence of recombination provides robust evidence for recognizing C. guttulatum as a novel species within the C. dracaenophilum species complex.</p></div>	https://treatment.plazi.org/id/827887CEFFCE7002B1BB10B2E6D7FEB2	Public Domain	No known copyright restrictions apply. See Agosti, D., Egloff, W., 2009. Taxonomic information exchange and copyright: the Plazi approach. BMC Research Notes 2009, 2:53 for further explanation.		Plazi	Norphanphoun, Chada;Guo, Shi-Qi;Wang, Yong	Norphanphoun, Chada, Guo, Shi-Qi, Wang, Yong (2025): Colletotrichum-guttulatum sp. - nov.: a novel species from southwestern China. Phytotaxa 717 (2): 165-176, DOI: 10.11646/phytotaxa.717.2.3, URL: https://doi.org/10.11646/phytotaxa.717.2.3
