identifier	taxonID	type	CVterm	format	language	title	description	additionalInformationURL	UsageTerms	rights	Owner	contributor	creator	bibliographicCitation
7E658ECD6A605ADE9B3076E142849DD6.text	7E658ECD6A605ADE9B3076E142849DD6.taxon	http://purl.org/dc/dcmitype/Text	http://rs.tdwg.org/ontology/voc/SPMInfoItems#GeneralDescription	text/html	en	Amphisphaeria hydei M. C. Samar.	<div><p>Amphisphaeria hydei M. C. Samar., New Zealand J. Bot. 62 (2–3): 262 (2024)</p><p>Fig. 6</p><p>Description.</p><p>Saprobic on a dead branch of Casearia sp. ( Salicaceae). Sexual morph: Ascomata 230–280 × 175–215 μm (x ̄ = 264 × 198 μm, n = 5), immersed, visible dark brown to black dots, solitary, scattered, in cross-section, sub-globose to conical with mostly flattened base. Ostioles centric, prominent, ostiolar canal filled with periphyses, 1.3–2.2 μm (x ̄ = 1.7 μm, n = 15). Peridium 6–12 μm (x ̄ = 8.7 μm, n = 10) wide, multi-layered, outer layer thin, tightly attached to host tissue, comprising dark brown, thick-walled cells of textura angularis, inner layer thick, composed of hyaline, thin-walled cells of textura angularis. Paraphyses 2.5–4.5 μm (x ̄ = 3.4 μm, n = 20) wide, longer than asci, cellular, septate, constricted at septa, branched, guttulate, embedded in a gelatinous matrix. Asci 60–80 × 5.5–9 μm (x ̄ = 71.5 × 7 μm, n = 20), eight-spored, unitunicate, cylindrical, J- in Melzer’s reagent, apically rounded. Ascospores 10–16 × 3–5 μm (x ̄ = 12.2 × 3.9 μm, n = 35), L / W 3.1, uniseriate or 2 - seriate, brown, ellipsoid, one-septate, constricted at the septum, guttulate, smooth-walled, lacking a mucilaginous sheath. Asexual morph: Undetermined.</p><p>Material examined.</p><p>Thailand • Lamphun Province, Mueang District, Sri Bua Ban Sub-District, on a dead branch of Casearia sp. ( Salicaceae) attached to the host, 24 August 2023, MC. Samarakoon, MC 22-106 (MFLU 26-0123; CMUB 40124) .</p><p>Additional sequence.</p><p>PX 513828 (tef 1 - α; MFLU 26-0123).</p><p>Notes.</p><p>In the combined ITS – LSU – rpb 2 – tub 2 phylogeny, collection MC 22-106 clustered with A. hydei with strong statistical support (100 % ML). The ITS, LSU, rpb 2, and tub 2 sequences of MC 22-106 showed 99 % similarity to those of A. hydei CMUB 40016 with 1 / 552, 0 / 933, 0 / 1005, and 0 / 1046 gaps, respectively. The ITS and LSU sequences of MC 22-106 are similar to those of A. yunnanensis KUMCC 19-0188 (89 %, 15 / 589 gaps and 97 %, 3 / 944 gaps). The rpb 2 and tub 2 sequences of A. hydeimucosa HKAS 132411 show 86 % (9 / 1052 gaps) and 87 % (28 / 1063 gaps) similarity to MC 22-106, respectively. Amphisphaeria hydei was originally described by Samarakoon (2024) from a dead branch. The new collection is morphologically similar to the type specimen in ascus shape and general dimensions but differs in having smaller asci (60–80 × 5.5–9 μm vs. 80–120 × 6–11 μm). Based on concordant morphological characters and high molecular sequence similarity, MC 22-106 is identified as A. hydei . This collection represents the first record of A. hydei from Casearia sp. ( Salicaceae) and is therefore reported here as a new host record.</p></div>	https://treatment.plazi.org/id/7E658ECD6A605ADE9B3076E142849DD6	Public Domain	No known copyright restrictions apply. See Agosti, D., Egloff, W., 2009. Taxonomic information exchange and copyright: the Plazi approach. BMC Research Notes 2009, 2:53 for further explanation.		Pensoft via Plazi	Samarakoon, Milan C.;Manawasinghe, Ishara S.	Samarakoon, Milan C., Manawasinghe, Ishara S. (2026): Additions to the Xylariomycetidae (Sordariomycetes) fungal flora from northern Thailand: four novel Amphisphaeria species. MycoKeys 132: 63-90, DOI: 10.3897/mycokeys.132.188390
320AFADE6AE5556099888E2A27856F27.text	320AFADE6AE5556099888E2A27856F27.taxon	http://purl.org/dc/dcmitype/Text	http://rs.tdwg.org/ontology/voc/SPMInfoItems#GeneralDescription	text/html	en	Amphisphaeria longiostiolata M. C. Samar. 2026	<div><p>Amphisphaeria longiostiolata M. C. Samar. sp. nov.</p><p>Fig. 2</p><p>Etymology.</p><p>The specific epithet “longiostiolata” refers to the long ostiolar neck.</p><p>Holotype.</p><p>MFLU 26-0120.</p><p>Description.</p><p>Saprobic on an unidentified dicotyledonous dead branch. Sexual morph: Ascomata 200–260 × 200–240 μm (x ̄ = 238 × 223 μm, n = 5), immersed, visible as raised dark black-brown area, center black dots, solitary, scattered, in cross-section, globose to sub-globose. Ostioles centric, prominent, long (95–120 μm), conical ostiolar neck, ostiolar canal periphysate. Peridium 15.5–21 μm (x ̄ = 19.6 μm, n = 10) wide, multi-layered, outer layer thick, tightly attached to host tissue, comprising dark brown to black, thick-walled cells of textura angularis, inner layer thin, composed of hyaline, thin-walled cells of textura angularis. Paraphyses 3–5 μm (x ̄ = 4.1 μm, n = 20) wide, longer than asci, cellular, septate, branched, guttulate, embedded in a gelatinous matrix. Asci 80–100 × 6–9.5 μm (x ̄ = 89.5 × 7.6 μm, n = 20), eight-spored, unitunicate, cylindrical, J- in Melzer’s reagent, apically rounded. Ascospores 10.5–15.5 × 4–6.5 μm (x ̄ = 12.6 × 5 μm, n = 35), L / W 2.5, uniseriate, hyaline when young, light brown when mature, ellipsoid, one-septate (rarely two-septate), constricted at septa, bi-guttulate, smooth-walled, mucilaginous sheath 1.5–4.6 μm (x ̄ = 3.1 μm, n = 20). Asexual morph: Undetermined.</p><p>Material examined.</p><p>Thailand • Lamphun Province, Mueang District, on an unidentified dead dicotyledonous branch attached to the host, 22 October 2022, MC. Samarakoon, MC 22-023 (Holotype MFLU 26-0120; Isotype CMUB 40125) .</p><p>Additional sequence.</p><p>PX 513825 (tef 1 - α; MFLU 26-0120).</p><p>Notes.</p><p>In the combined ITS – LSU – rpb 2 – tub 2 phylogeny, collection MC 22-023 formed an independent clade sister to A. shangrilaensis and A. sorbi with robust statistical support (85 % MP / 99 % ML / 1.00 PP). The ITS sequence from MC 22-023 is similar to that of A. neoaquatica (MFLU 15-0077; 92 %, 6 / 452 gaps), A. flava (MFLU 18-0102; 92 %, 11 / 461 gaps), A. karsti (GZAAS 20-0147; 91 %, 6 / 459 gaps), and A. shangrilaensis (HKAS 130272; 87 %, 25 / 616 gaps), while the LSU sequence is similar to that of A. guttulata (MFLU 22-0078; 96 %, 6 / 934 gaps), A. thailandica (MFLU 18-0794; 96 %, 7 / 927 gaps), A. sambuci (CBS 131707; 93 %, 20 / 1170 gaps), and A. fuckelii (CBS 140409; 92 %, 42 / 1195 gaps). The rpb 2 and tub 2 sequences of MC 22-023 are similar to those of A. hydeimucosa (HKAS 132411; 87 %, 0 / 1062 gaps and 87 %, 26 / 1118 gaps), A. hydei (CMUB 40016; 87 %, 9 / 1014 gaps, and MFLU 23-0412; 86 %, 30 / 1087 gaps), and A. fuckelii (CBS 140409; 85 %, 5 / 1056 gaps and 90 %, 10 / 825 gaps). The collection MC 22-023 has solitary, immersed ascomata with a two-layered peridium and unitunicate asci, which are morphologically similar to those of other species in the genus. Amphisphaeria sorbi, A. thailandica, and A. magna possess apical ring J- in Melzer’s reagent, similar to MC 22-023, and differ from A. shangrilaensis in having an apical ring J + in Melzer’s reagent (Liu et al. 2015; Dissanayake et al. 2024). Compared to the similar taxa, collection MC 22-023 has a distinct, long, conical ostiolar neck. Amphisphaeria sorbi and A. magna have larger ascomata compared to MC 22-023 (350–380 × 450–505 μm and 300–350 μm × 450–520 μm vs. 200–260 × 200–240 μm). The species remain as ‘ lepteutypa-like’, as ‘ Lepteutypa sabalicola ’ and ‘ L. tropicalis ’ possess immersed, subglobose ascomata and apical rings J- in Melzer’s reagent; however, short ostiolar neck, asci size, and appearance of ascomata differ from MC 22-023 (Barr 1993; Dulymamode et al. 2001). Based on its distinct morphology and phylogeny, the new collection MC 22-023 is introduced here as a new species, A. longiostiolata .</p></div>	https://treatment.plazi.org/id/320AFADE6AE5556099888E2A27856F27	Public Domain	No known copyright restrictions apply. See Agosti, D., Egloff, W., 2009. Taxonomic information exchange and copyright: the Plazi approach. BMC Research Notes 2009, 2:53 for further explanation.		Pensoft via Plazi	Samarakoon, Milan C.;Manawasinghe, Ishara S.	Samarakoon, Milan C., Manawasinghe, Ishara S. (2026): Additions to the Xylariomycetidae (Sordariomycetes) fungal flora from northern Thailand: four novel Amphisphaeria species. MycoKeys 132: 63-90, DOI: 10.3897/mycokeys.132.188390
3C402932B772509BB70F9C876461EEDC.text	3C402932B772509BB70F9C876461EEDC.taxon	http://purl.org/dc/dcmitype/Text	http://rs.tdwg.org/ontology/voc/SPMInfoItems#GeneralDescription	text/html	en	Amphisphaeria oleae W. Li Li, R. R. Liang & Jian K. Liu	<div><p>Amphisphaeria oleae W. Li Li, R. R. Liang &amp; Jian K. Liu, J. Fungi 10 (3, no. 189): 10 (2024)</p><p>Fig. 7</p><p>Description.</p><p>Saprobic on a dead branch of Prunus sp. ( Rosaceae). Sexual morph: Ascomata 540–640 × 250–295 μm (x ̄ = 591 × 275 μm, n = 5), immersed, visible as black dots, solitary, scattered, in cross-section, conical with mostly flattened base. Ostioles centric, prominent, wide, ostiolar canal filled with dense periphyses, 1–2 μm (x ̄ = 1.5 μm, n = 20). Peridium 12–20 μm (x ̄ = 16.4 μm, n = 10) wide, wider at the corners up to 27 μm, multi-layered, outer layer tightly attached to host tissue, comprising reddish brown, thick-walled cells of textura angularis, easy to disintegrate when mature, inner layer thick, composed of hyaline, thin-walled cells of textura angularis. Paraphyses 2.4–4.5 μm (x ̄ = 3.3 μm, n = 20) wide, longer than asci, cellular, septate, constricted at septa, branched, guttulate, embedded in a gelatinous matrix. Asci 105–155 × 6–8.5 μm (x ̄ = 130.5 × 7.2 μm, n = 20), eight-spored, unitunicate, cylindrical, with a 2.2–3 × 0.5–1.2 μm (x ̄ = 2.5 × 0.9 μm, n = 8), discoid, apical ring, J + in Melzer’s reagent, apically rounded. Ascospores 14–19 × 5–6.5 μm (x ̄ = 16.2 × 5.8 μm, n = 35), L / W 2.8, uniseriate, brown, ellipsoid, one-septate, constricted at the septum, guttulate, smooth-walled. Asexual morph: Undetermined.</p><p>Material examined.</p><p>Thailand • Chiang Mai Province, Mae Rim District, Mae Raem, on a dead branch of Prunus sp. ( Rosaceae) attached to the host, 17 October 2023, MC. Samarakoon, MC 22-111 (MFLU 26-0124; CMUB 40142) .</p><p>Additional sequence.</p><p>PX 513829 (tef 1 - α; MFLU 26-0124).</p><p>Notes.</p><p>In the combined ITS – LSU – rpb 2 – tub 2 phylogeny, MC 22-111 clustered with Amphisphaeria oleae and A. xishuangbannaensis with strong statistical support (100 % ML). The ITS and LSU sequences from MC 22-111 are similar to those of A. oleae (CGMCC 3.24959; 99 %, 1 / 581 gap, 0 / 870 gaps), A. uniseptata (HKAS 112607; 100 %, 0 / 566 gaps, 98 %, 22 / 1185 gaps), and A. xishuangbannaensis (KUNCC 23-15524; 97 %, 14 / 556 gaps, 99 %, 6 / 694 gaps). The rpb 2 sequence of MC 22-111 is similar to that of A. oleae (CGMCC 3.24959; 99 %, 0 / 1048 gaps), A. uniseptata (GZCC 20-0498; 100 %, 0 / 987 gaps and CBS 114967; 99 %, 0 / 832 gaps), and A. camelliae (HKAS 107021; 97 %, 0 / 972 gaps). Collection MC 22-111 is characterized by having immersed ascomata, eight-spored, unitunicate, cylindrical asci, and uniseriate, brown, ellipsoid ascospores, consistent with the genus Amphisphaeria . Amphisphaeria oleae, A. xishuangbannaensis, and MC 22-111 have immersed ascomata visible as black dots but differ in shapes, having conical ascomata with mostly flattened bases versus globose to subglobose (Dissanayake et al. 2024; Li et al. 2024). Collection MC 22-111 has a discoid apical ring and is J + in Melzer’s reagent, similar to A. oleae, the type species introduced by Li et al. (2024) on branches of Olea europaea from China, but A. xishuangbannaensis has a J- apical ring in Melzer’s reagent. Following the similar morphologies and phylogenies, A. oleae, A. uniseptata, and A. xishuangbannaensis share close morphologies and phylogenies. However, based on the closest morphology and phylogeny, MC 22-111 is taxonomically placed in A. oleae, and this is the first report of this species associated with Prunus sp. and the first report from Thailand.</p></div>	https://treatment.plazi.org/id/3C402932B772509BB70F9C876461EEDC	Public Domain	No known copyright restrictions apply. See Agosti, D., Egloff, W., 2009. Taxonomic information exchange and copyright: the Plazi approach. BMC Research Notes 2009, 2:53 for further explanation.		Pensoft via Plazi	Samarakoon, Milan C.;Manawasinghe, Ishara S.	Samarakoon, Milan C., Manawasinghe, Ishara S. (2026): Additions to the Xylariomycetidae (Sordariomycetes) fungal flora from northern Thailand: four novel Amphisphaeria species. MycoKeys 132: 63-90, DOI: 10.3897/mycokeys.132.188390
6065795F71A858748CD263D523C530D8.text	6065795F71A858748CD263D523C530D8.taxon	http://purl.org/dc/dcmitype/Text	http://rs.tdwg.org/ontology/voc/SPMInfoItems#GeneralDescription	text/html	en	Amphisphaeria planibasalis M. C. Samar. 2026	<div><p>Amphisphaeria planibasalis M. C. Samar. sp. nov.</p><p>Fig. 3</p><p>Etymology.</p><p>The specific epithet “ planibasalis ” refers to the flat base of the ascomata.</p><p>Holotype.</p><p>MFLU 26-0119.</p><p>Description.</p><p>Saprobic on an unidentified dicotyledonous dead branch. Sexual morph: Ascomata 345–450 × 120–235 μm (x ̄ = 416 × 183 μm, n = 7), immersed, visible as raised, black dots, solitary, rarely aggregated (two ascomata), scattered, in cross-section, conical with mostly flattened base. Ostioles centric, prominent, conical, ostiolar canal short and periphysate, 1.1–1.8 μm (x ̄ = 1.4 μm, n = 15). Peridium 6.5–10 μm (x ̄ = 8.5 μm, n = 10) wide, wider at the corners up to 18 μm, multi-layered, outer layer tightly attached to host tissue, comprising reddish brown, thick-walled cells of textura angularis, inner layer composed of hyaline, thin-walled cells of textura angularis. Paraphyses 2.7–5.3 μm (x ̄ = 3.6 μm, n = 20) wide, longer than asci, cellular, septate, branched, guttulate, embedded in a gelatinous matrix. Asci 110–145 × 8.5–14 μm (x ̄ = 127 × 11.2 μm, n = 20), eight-spored, unitunicate, cylindrical, with a 3–4 × 0.6–1.1 μm (x ̄ = 3.5 × 0.9 μm, n = 10), discoid, apical ring, J + in Melzer’s reagent, apically rounded. Ascospores 13.5–19.5 × 5–7.5 μm (x ̄ = 16.2 × 5.8 μm, n = 35), L / W 2.8, uniseriate, hyaline when young, light to dark brown when mature, ellipsoid, one-septate, constricted at septum, prominent bi-guttulate when immature, smooth-walled, thin mucilaginous sheath 0.7–2 μm (x ̄ = 1.4 μm, n = 20). Asexual morph: Undetermined.</p><p>Material examined.</p><p>Thailand • Lamphun Province, Mueang District, on an unidentified dead dicotyledonous branch attached to the host, 22 October 2022, MC. Samarakoon, MC 22-014 (Holotype MFLU 26-0119; Isotype CMUB 40143) .</p><p>Additional sequence.</p><p>PX 513824 (tef 1 - α; MFLU 26-0119).</p><p>Notes.</p><p>In the combined ITS – LSU – rpb 2 – tub 2 phylogeny, collection MC 22-014 formed a distinct branch. The ITS sequence of MC 22-014 is similar to that of A. umbrina (PRA - JV 24328; 90 %, 17 / 596 gaps), A. flava (MFLU 18-0102; 90 %, 17 / 598 gaps), A. ailaoshanensis (KUNCC 23-15520; 90 %, 24 / 575 gaps), and A. fuckelii (CBS 140409; 89 %, 12 / 595 gaps), while the LSU is similar to that of A. hydei (CMUB 40016; 98 %, 1 / 896 gap), A. guttulata (MFLU 22-0078; 98 %, 2 / 892 gaps), A. mesuae (MFLU 25-0069; 97.9 %, 4 / 900 gaps), and A. thailandica (MFLU 18-0794; 97 %, 1 / 906 gap). The rpb 2 sequence shows similarity to A. fuckelii (CBS 140409; 86 %, 0 / 1055 gap), A. qujingensis (KUMCC 19-0187; 86 %, 0 / 1049 gap), and A. thailandica (MFLU 18-0794; 85 %, 5 / 781 gaps). Collection MC 22-014 is similar to other taxa by having immersed ascomata, ellipsoid ascospores, and a J + apical ring in Melzer’s reagent (the apical ring of A. hibiscicola is not described) (Sun et al. 2025). Amphisphaeria flava and A. umbrina differ from MC 22-014 by the presence of a yellow halo on the substrate around the neck area and a clypeus, respectively. Amphisphaeria conica (current name A. umbrina) was originally introduced as Sphaeropsis conica as the generic type, highlighting the conical shape of the ascomata and the flat base. The ascospores of A. flava possess a sheath similar to that of MC 22-014. However, the prominent conical ascomata of MC 22-014 differ from the globose to subglobose ascomata found in the other species. ‘ Lepteutypa hexagonalis, ’ which lacks molecular data, resembles MC 22-014 in having immersed, conical-pyramidal ascomata with a flattened base that are triangular, occurring singly or in pairs, and possessing a J +, discoid apical ring (Goh and Hyde 1997). However, it differs in having larger ascomata (700–800 × 600–700 µm) and fusiform, three-septate ascospores with 6–7 longitudinal ridges (Goh and Hyde 1997). Based on morphology and phylogeny, collection MC 22-014 is identified as a new species, A. planibasalis .</p></div>	https://treatment.plazi.org/id/6065795F71A858748CD263D523C530D8	Public Domain	No known copyright restrictions apply. See Agosti, D., Egloff, W., 2009. Taxonomic information exchange and copyright: the Plazi approach. BMC Research Notes 2009, 2:53 for further explanation.		Pensoft via Plazi	Samarakoon, Milan C.;Manawasinghe, Ishara S.	Samarakoon, Milan C., Manawasinghe, Ishara S. (2026): Additions to the Xylariomycetidae (Sordariomycetes) fungal flora from northern Thailand: four novel Amphisphaeria species. MycoKeys 132: 63-90, DOI: 10.3897/mycokeys.132.188390
47CCC492615B5342BCE51BDB1814A5EC.text	47CCC492615B5342BCE51BDB1814A5EC.taxon	http://purl.org/dc/dcmitype/Text	http://rs.tdwg.org/ontology/voc/SPMInfoItems#GeneralDescription	text/html	en	Amphisphaeria pterocarpi Z. L. Tun & K. D. Hyde	<div><p>Amphisphaeria pterocarpi Z. L. Tun &amp; K. D. Hyde, MycoKeys 125: 1–31 (2025)</p><p>Fig. 8</p><p>Description.</p><p>Saprobic on an unidentified dicotyledonous dead branch. Sexual morph: Ascomata 320–430 × 155–205 μm (x ̄ = 373 × 176 μm, n = 5), immersed, visible black dots, solitary, scattered, in cross-section, oblate or depressed-globose. Ostioles centric, prominent, wide, ostiolar canal filled with dense periphyses, 1–2.2 μm (x ̄ = 1.6 μm, n = 20), occasionally ostiolar neck depressed when mature. Peridium 13–26 μm (x ̄ = 21 μm, n = 10) wide, multi-layered, comprising reddish brown, thick-walled cells of textura angularis, inner layer thick, composed of hyaline, thin-walled cells of textura angularis. Paraphyses 2.4–5.5 μm (x ̄ = 4.1 μm, n = 20) wide, longer than asci, cellular, septate, constricted at septa, branched, guttulate, embedded in a gelatinous matrix. Asci 95–150 × 10.5–15 μm (x ̄ = 126 × 12.5 μm, n = 20), eight-spored, unitunicate, cylindrical, with a 2.5–3.5 × 0.7–1.6 μm (x ̄ = 3.1 × 1.1 μm, n = 8), discoid, apical ring, J + in Melzer’s reagent, apically rounded. Ascospores 18.5–22 × 5.5–7.5 μm (x ̄ = 20.5 × 6.7 μm, n = 35), L / W 3.1, uniseriate to overlapping biseriate, brown, ellipsoid, with 1 median, slightly constricted euseptum and two distosepta, guttulate, smooth-walled, mucilaginous sheath 1.3–2 μm (x ̄ = 1.6 μm, n = 15) when immature, thinner or lacking when mature. Asexual morph: Undetermined.</p><p>Material examined.</p><p>Thailand • Chiang Mai Province, Mae Rim District, Mae Raem, on an unidentified dicotyledonous dead branch attached to the host, 17 October 2023, MC. Samarakoon, MC 22-116 (MFLU 26-0125; CMUB 40144) .</p><p>Additional sequence.</p><p>PX 513830 (tef 1 - α; MFLU 26-0125).</p><p>Notes.</p><p>In the combined ITS – LSU – rpb 2 – tub 2 phylogeny, MC 22-116 clusters within the A. pterocarpi clade with strong support (98 % ML). The ITS sequence of MC 22-116 is similar to that of A. pterocarpi (MFLU 25-0072; 99 %, 1 / 554 gap) and A. curvaticonidia (HKAS 102288; 93 %, 28 / 578 gaps), while the LSU sequence shows close similarity to A. pterocarpi (MFLU 25-0072; 99 %, 2 / 1062 gaps), A. guizhouensis (GMBC 5901; 97 %, 7 / 1054 gaps), A. schimae (MFLU 25-0070; 94 %, 15 / 1075 gaps), and A. fuckelii (CBS 140409; 94 %, 17 / 1077 gaps). The rpb 2 sequence is most similar to A. curvaticonidia (MFLU 18-0789; 95 %, 0 / 882 gap) and A. parvispora (MFLU 18-0767; 86 %, 2 / 1050 gaps). The tub 2 sequence shows similarity to that of A. fuckelii (CBS 140409; 87 %, 33 / 1036 gaps) and A. hydeimucosa (HKAS 132411; 89 %, 13 / 839 gaps). However, the tub 2 sequence also shows 89 % similarity (13 / 839 gaps) to Byssosphaeria vaginata UESTCC: 23.0224 (acc. PP 800890), a species placed in Melanommataceae ( Pleosporales, Dothideomycetes) by Yu et al. (2025), indicating that this sequence may need re-evaluation. Amphisphaeria pterocarpi was introduced by Tun et al. (2025) from recently dead branches of Pterocarpus rotundifolius ( Fabaceae) collected in Chiang Rai Province, Thailand. Collection MC 22-116 shares the key morphological characteristics of the type specimen (MFLU 25-0073) of A. pterocarpi in having immersed, solitary ascomata; a peridium composed of textura angularis cells; eight-spored unitunicate cylindrical asci with a discoid apical ring that is J + in Melzer’s reagent; and brown ellipsoid ascospores surrounded by a mucilaginous sheath. The new collection has oblate to depressed-globose ascomata, whereas the type specimen bears globose to subglobose ascomata, likely representing a difference in maturity or host substrate. Based on morphology and phylogeny, MC 22-116 is identified as A. pterocarpi .</p></div>	https://treatment.plazi.org/id/47CCC492615B5342BCE51BDB1814A5EC	Public Domain	No known copyright restrictions apply. See Agosti, D., Egloff, W., 2009. Taxonomic information exchange and copyright: the Plazi approach. BMC Research Notes 2009, 2:53 for further explanation.		Pensoft via Plazi	Samarakoon, Milan C.;Manawasinghe, Ishara S.	Samarakoon, Milan C., Manawasinghe, Ishara S. (2026): Additions to the Xylariomycetidae (Sordariomycetes) fungal flora from northern Thailand: four novel Amphisphaeria species. MycoKeys 132: 63-90, DOI: 10.3897/mycokeys.132.188390
EC7001025E39543C8DB5686C62415505.text	EC7001025E39543C8DB5686C62415505.taxon	http://purl.org/dc/dcmitype/Text	http://rs.tdwg.org/ontology/voc/SPMInfoItems#GeneralDescription	text/html	en	Amphisphaeria sanpakiaensis M. C. Samar. 2026	<div><p>Amphisphaeria sanpakiaensis M. C. Samar. sp. nov.</p><p>Fig. 4</p><p>Etymology.</p><p>The specific epithet “ sanpakiaensis ” refers to the San Pa Kia Highland Agricultural Research Station in Chiang Mai Province, Thailand, where the holotype specimen was collected.</p><p>Holotype.</p><p>MFLU 26-0121.</p><p>Description.</p><p>Saprobic on an unidentified dicotyledonous dead branch. Sexual morph: Ascomata 200–320 × 155–245 μm (x ̄ = 262 × 196.5 μm, n = 5), immersed, visible as black dots, solitary, scattered, in cross-section, globose to sub-globose. Ostioles centric, prominent, ostiolar canal filled with white amorphous tissues. Peridium 24–36 μm (x ̄ = 30.5 μm, n = 10) wide at immature stage, multi-layered, outer layer thin, tightly attached to host tissue, comprising brown, thick-walled cells of textura epidermoidea, inner layer thick, composed of hyaline, thin-walled cells of textura epidermoidea, 12–18 μm (x ̄ = 14.5 μm, n = 10) wide at mature stage, easy to disintegrate. Paraphyses 3.5–6.5 μm (x ̄ = 4.7 μm, n = 20) wide, longer than asci, cellular, septate, branched, guttulate, embedded in a gelatinous matrix. Asci 90–120 × 7.5–10 μm (x ̄ = 104 × 9.2 μm, n = 20), eight-spored, unitunicate, cylindrical, with a 3–3.7 × 1–1.5 μm (x ̄ = 3.4 × 1.3 μm, n = 7), discoid, apical ring, J + in Melzer’s reagent, apically rounded. Ascospores 17.5–20.5 × 5.5–7.5 μm (x ̄ = 19 × 6.6 μm, n = 35), L / W 2.9, uniseriate, hyaline, aseptate with large central guttule when young, brown, ellipsoid with slightly pointed ends, one-septate, slightly constricted at septum, bi-guttulate, smooth-walled when mature, mucilaginous caps at both ends when immature. Asexual morph: Undetermined.</p><p>Material examined.</p><p>Thailand • Chiang Mai Province, Chiang Dao District, San Pa Kia Highland Agricultural Research Station, on an unidentified dicotyledonous dead branch attached to the host, 13 February 2023, MC. Samarakoon, MC 22-036 (Holotype MFLU 26-0121; Isotype CMUB 40145) .</p><p>Additional sequence.</p><p>PX 513826 (tef 1 - α; MFLU 26-0121).</p><p>Notes.</p><p>In the combined ITS – LSU – rpb 2 – tub 2 phylogeny, MC 22-036 forms an independent clade sister to A. zhaotongensis (100 % MP / 100 % ML / 1.00 PP). The ITS sequence is similar to that of A. hydeimucosa (HKAS 132411; 92 %, 11 / 574 gaps), A. ailaoshanensis (KUNCC 23-15520; 90 %, 15 / 567 gaps), A. yunnanensis (KUMCC 19-0188; 89 %, 17 / 591 gaps), and A. fuckelii (CBS 140409; 88 %, 27 / 599 gaps). The LSU sequence is similar to that of A. guttulata (MFLU 22-0078; 95 %, 18 / 1160 gaps), A. hydeimucosa (HKAS 132411; 95 %, 16 / 1147 gaps), and A. schimae (MFLU 25-0070; 95 %, 17 / 1147 gaps). The rpb 2 sequence is similar to that of A. fuckelii (CBS 140409; 87 %, 0 / 1052 gaps), A. qujingensis (KUMCC 19-0187; 86 %, 0 / 1049 gaps), and A. hydeimucosa (HKAS 132411; 85 %, 3 / 1055 gaps), while the tub 2 sequence is similar to A. hydeimucosa (HKAS 132411; 86 %, 33 / 1089 gaps) and A. hydei (MFLU 23-0412; 86 %, 35 / 1092 gaps). Collection MC 22-036 possesses immersed ascomata, cellular, septate paraphyses, eight-spored, unitunicate cylindrical asci, and septate ascospores, which are morphologically similar to those of other species in the genus. However, MC 22-036 has a textura epidermoidea peridium and ellipsoid, slightly pointed ends, one-septate, mature ascospores with mucilaginous caps at both ends, distinct from other Amphisphaeria species. The majority of the Amphisphaeria species have textura angularis cells in their peridium, and a few of them have textura intricata or textura prismatica, but none of them have textura epidermoidea. Based on its morphology and multigene phylogeny, the collection MC 22-036 is introduced as A. sanpakiaensis .</p></div>	https://treatment.plazi.org/id/EC7001025E39543C8DB5686C62415505	Public Domain	No known copyright restrictions apply. See Agosti, D., Egloff, W., 2009. Taxonomic information exchange and copyright: the Plazi approach. BMC Research Notes 2009, 2:53 for further explanation.		Pensoft via Plazi	Samarakoon, Milan C.;Manawasinghe, Ishara S.	Samarakoon, Milan C., Manawasinghe, Ishara S. (2026): Additions to the Xylariomycetidae (Sordariomycetes) fungal flora from northern Thailand: four novel Amphisphaeria species. MycoKeys 132: 63-90, DOI: 10.3897/mycokeys.132.188390
924EC0327B345BC0B2826555842C951D.text	924EC0327B345BC0B2826555842C951D.taxon	http://purl.org/dc/dcmitype/Text	http://rs.tdwg.org/ontology/voc/SPMInfoItems#GeneralDescription	text/html	en	Amphisphaeria sribuabanensis M. C. Samar. 2026	<div><p>Amphisphaeria sribuabanensis M. C. Samar. sp. nov.</p><p>Fig. 5</p><p>Etymology.</p><p>The specific epithet “ sribuabanensis ” refers to the Sri Bua Ban Sub-District in Chiang Mai Province, Thailand, where the holotype specimen was collected.</p><p>Holotype.</p><p>MFLU 26-0122.</p><p>Description.</p><p>Saprobic on an unidentified dicotyledonous dead branch. Sexual morph: Ascomata 385–525 × 410–490 μm (x ̄ = 456 × 440 μm, n = 5), immersed, visible as dark brown to black dots, solitary, scattered, in cross-section, globose to sub-globose. Ostioles centric, prominent, ostiolar canal filled with periphyses, 1.3–3 μm (x ̄ = 1.6 μm, n = 15), bright yellow at the top. Peridium 15–28 μm (x ̄ = 21 μm, n = 10) wide, multi-layered, outer layer thin, tightly attached to host tissue, comprising dark brown, thick-walled cells of textura angularis, inner layer thick, composed of light brown to hyaline, thin-walled cells of textura angularis. Paraphyses 2.3–4.5 μm (x ̄ = 3.3 μm, n = 20) wide, longer than asci, cellular, septate, constricted at septa, branched, guttulate, embedded in a gelatinous matrix. Asci 140–175 × 12.5–19 μm (x ̄ = 158 × 14.7 μm, n = 20), eight-spored, unitunicate, cylindrical, with a 4.3–5 × 1.5–2 μm (x ̄ = 4.8 × 1.8 μm, n = 8), discoid, apical ring, J + in Melzer’s reagent, apically rounded. Ascospores 19–26 × 6.5–11 μm (x ̄ = 22.2 × 8.6 μm, n = 35), L / W 2.6, uniseriate, hyaline, aseptate with large central guttule when immature, brown, ellipsoid, three-septate, constricted at median septum, guttulate, smooth-walled when mature. Asexual morph: Undetermined.</p><p>Material examined.</p><p>Thailand • Lamphun Province, Mueang District, Sri Bua Ban Sub-District, on an unidentified dicotyledonous dead branch attached to the host, 24 August 2023, MC. Samarakoon, MC 22-101 (Holotype MFLU 26-0122; Isotype CMUB 40146) .</p><p>Additional sequence.</p><p>PX 513827 (tef 1 - α; MFLU 26-0122).</p><p>Notes.</p><p>In the combined ITS – LSU – rpb 2 – tub 2 phylogeny, MC 22-101 forms a sister clade to the A. verniciae and A. falcata clade with strong statistical support (100 % MP / 100 % ML / 1.00 PP). The ITS, LSU, rpb 2, and tub 2 sequences of MC 22-101 are similar to those of A. fuckelii (CBS 140409; 89 %, 18 / 591 gaps; 93 %, 17 / 1165 gaps; 12 / 1058 gaps; and 84 %, 36 / 1016 gaps). The ITS sequence of MC 22-101 is similar to that of A. curvaticonidia (MFLU 18-0789; 90 %, 19 / 585 gaps) and A. qujingensis (KUMCC 19-0187; 89 %, 17 / 591 gaps), while the LSU sequence is similar to that of A. pterocarpi (MFLU 25-0072; 94 %, 15 / 1156 gaps), A. guizhouensis (GMBC 5901; 94 %, 11 / 1144 gaps), and A. schimae (MFLU 25-0070; 94 %, 13 / 1163 gaps). The rpb 2 sequence of MC 22-101 is similar to that of A. parvispora (MFLU 18-0767; 83 %, 4 / 1051 gaps), A. qujingensis (KUMCC 19-0187; 82 %, 12 / 1055 gaps), and A. chiangmaiensis (CMUB 40017; 82 %, 8 / 1012 gaps), while the tub 2 sequence is similar to that of A. camelliae (HKAS 107021; 85 %, 35 / 1014 gaps), A. verniciae (UESTCC: 23.0122; 89 %, 5 / 762 gaps), and A. micheliae (HKAS 107012; 89 %, 8 / 767 gaps). Collection MC 22-101 has immersed ascomata, a multi-layered peridium, cellular, septate paraphyses, eight-spored, unitunicate, cylindrical asci, and septate ascospores, which are similar to those of the genus Amphisphaeria . Collection MC 22-101 and A. verniciae share similar morphologies, having globose to subglobose, immersed ascomata; a peridium with textura angularis cells; brown, three-septate mature ascospores; and lacking mucilaginous sheaths (Li et al. 2024). However, MC 22-101 has larger ascomata (456 × 440 μm vs. 150 × 170 µm), with bright yellow at the top of the ostiolar canal, larger asci (158 × 14.7 μm vs. 130 × 11 µm), and ascospores (22.2 × 8.6 μm vs. 17 × 6.5 µm). Amphisphaeria verniciae lacks information about the apical ring in Melzer’s reagent. Amphisphaeria falcata was described only from the asexual morph, which limits detailed morphological comparison (Chang et al. 2025). Several ‘ Lepteutypa ’ species have three-septate ascospores, which are similar to collection MC 22-101. ‘ Lepteutypa sabalicola ’ and ‘ L. tropicalis ’ possess a J- apical ring in Melzer’s reagent and smaller ascomata compared to MC 22-101 (Barr 1993; Dulymamode et al. 2001). ‘ Lepteutypa hederae ’ and MC 22-101 share overlapping shapes and sizes of ascomata (500 × 400–800 μm vs. 456 × 440 μm, globose to subglobose), asci (130–190 × 15 μm vs. 140–175 × 12.5–19 μm), and ascospores (19–30 × 8–11 μm vs. 19–26 × 6.5–11 μm) and differ in having a J- apical ring in Melzer’s reagent (Rappaz 1995). ‘ Lepteutypa hexagonalis ’ has a conical-pyramidal, flattened base; triangular, larger ascomata (700–800 × 600–700 μm); textura intricata cells in the outer layer of the peridium; and fusiform ascospores with 6–7 longitudinal ridges (Goh and Hyde 1997). Based on the distinct morphology and phylogeny, collection MC 22-101 is introduced as a new species, A. sribuabanensis .</p></div>	https://treatment.plazi.org/id/924EC0327B345BC0B2826555842C951D	Public Domain	No known copyright restrictions apply. See Agosti, D., Egloff, W., 2009. Taxonomic information exchange and copyright: the Plazi approach. BMC Research Notes 2009, 2:53 for further explanation.		Pensoft via Plazi	Samarakoon, Milan C.;Manawasinghe, Ishara S.	Samarakoon, Milan C., Manawasinghe, Ishara S. (2026): Additions to the Xylariomycetidae (Sordariomycetes) fungal flora from northern Thailand: four novel Amphisphaeria species. MycoKeys 132: 63-90, DOI: 10.3897/mycokeys.132.188390
