identifier	taxonID	type	CVterm	format	language	title	description	additionalInformationURL	UsageTerms	rights	Owner	contributor	creator	bibliographicCitation
3A0687CBFF8BFF80E45D700B11E4B002.text	3A0687CBFF8BFF80E45D700B11E4B002.taxon	http://purl.org/dc/dcmitype/Text	http://rs.tdwg.org/ontology/voc/SPMInfoItems#GeneralDescription	text/html	en	Plasmodium falciparum	<html xmlns:mods="http://www.loc.gov/mods/v3">
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            <p> P. falciparum culture and standard membrane feeding assays (SMFA) </p>
            <p> P. falciparum (NF54) was cultured in the lab [7, 8, 17] with 4% O + - type red blood cells in a complete Roswell Park Memorial Institute (RPMI) 1640 medium with 10% human AB + serum and 12.5 μg/ml of hypoxanthine in a candle jar at 37 °C. Ten, 15-day-old cultured  P. falciparum -infected cells were collected through centrifugation (300 g for 3 min), and about 100 μl of pellet were resuspended in 1.5 ml fresh AB + - type serum and mixed with 1.4 ml O + - type red blood cells, containing about 0.2% stage V gametocytes [16]. Each candidate compound was dissolved in dimethylsulfoxide (DMSO) and diluted with DMSO to obtain different concentrations. About 2 μl of candidate solution was mixed with 298 μl of  P. falciparum -infected blood prepared above and was used to feed 60–80 3–5-day-old An. gambiae with feeders covered with parafilm at 37 °C for 30 min. Te fed mosquitoes were maintained in an insectary with 10% sugar in water for 7 days in the insectary and dissected to obtain midguts. Te midguts were stained with 0.1% mercury dibromofluorescein disodium salt in phosphate-buffered saline (PBS), and the oocysts were counted under a light microscope. </p>
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	https://treatment.plazi.org/id/3A0687CBFF8BFF80E45D700B11E4B002	Public Domain	No known copyright restrictions apply. See Agosti, D., Egloff, W., 2009. Taxonomic information exchange and copyright: the Plazi approach. BMC Research Notes 2009, 2:53 for further explanation.		Plazi	Niu, Guodong;Wang, Xiaohong;Li, Jun	Niu, Guodong, Wang, Xiaohong, Li, Jun (2024): Leucinostatins target PlaSModIUM mitochondria to block malaria transmission. Parasites & Vectors (524) 17 (1): 1-9, DOI: 10.1186/s13071-024-06608-8, URL: https://doi.org/10.1186/s13071-024-06608-8
