identifier	taxonID	type	CVterm	format	language	title	description	additionalInformationURL	UsageTerms	rights	Owner	contributor	creator	bibliographicCitation
2B2B8798AC70D937DCAABBFFFC27F9A3.text	2B2B8798AC70D937DCAABBFFFC27F9A3.taxon	http://purl.org/dc/dcmitype/Text	http://rs.tdwg.org/ontology/voc/SPMInfoItems#GeneralDescription	text/html	en	Reofilinostoc matlalcueyense G. S. Hentschke, G. Garduño-Solórzano & M. Martínez-García	<div><p>Reofilinostoc matlalcueyense gen. et sp. nov. G. S. Hentschke, G. Garduño-Solórzano &amp; M. Martínez-García (Figures 1, 2)</p><p>The material collected in the field had a morphology resembling a mouse ear, of 0.4-1.2 cm in length (n=25) and a firm cartilaginous texture (Fig. 1C, D), colonies pale to dark green to brown with a thick peridermal layer yellowish (17-24 µm) where capturing a mass of filaments develop perpendicularly towards the interior of the thallus (Fig. 2 A, B). In solid cultures, after 3 to 4 weeks, warty colonies are enclosed in a firm mucilage formed by compacted filaments.</p><p>The filaments are moniliform, first uniseriate (Fig. 2D) and then non-seriate (Fig. 2C, I). Trichomes can reach dimensions greater than 25 isopolar cells (Fig. 2E). The terminal and intercalary vegetative cells are barrel-shaped, of 4.5-6.1 µm width and 4.0-6.0 µm length, and 3.3-3.6 µm width and 2.3-4.0 µm length, respectively. It is a population with a polymorphic life cycle. Heterocytes, both terminal and intercalary solitary, demonstrate an apoheterocytic propagation scheme (2F). Reproduction formed by akinetes, ellipsoid, 2.5-3.9 µm width and 3-0-6.0 µm length (Fig. 2G), which develop a giant vegetative cell that conceives a four-celled individual through an unequal division (Fig. 2H). It is sheath is evident (Fig. 2F).</p><p>Holotype specimen: Herbarium IZTA 1929 (IZTA), collected on May 5, 2022, from the stream of the melting snow of  Nevado de Toluca, State Mexico, Mexico.</p><p>Reference strain: F02 (OR 724089)/ Facultad de Estudios Superiores Iztacala, UNAM.</p><p>Type locality: MEXICO. Stream from the melting snow from Protected Natural Area Nevado de Toluca, Estado de Mexico.</p><p>Habitat: growing on andesite rock.</p><p>Etymology:  Reofilinostoc matlalcueyense (matlalcueyense, in nahuatl language, slope of the volcano),  Reofilinostoc,  Nostoc from stream.</p><p>Morphologically,  Reofilinostoc matlalcueyense is close to  Komarekiella atlantica,  Mojavia,  Desmonostoc,  Halotia and microscopic species of  Nostoc, although they differ in some aspects. The new genus has the aggregated filaments of microcolonies like  Mojavia and  Halotia and stages in it is life cycle resembling some microscopic  Nostoc species e.g.  N. punctiforme var. populorum . If only isolated stages of the life cycle of  Komarekiella were observed, it would be possible to incorrectly assume that those stages were one or more of these other taxa. Comparing the whole life cycle of  Komarekiella with other genera, it was observed that this taxon is indistinguishable from  Chlorogloeopsis, especially with regard to the unequal germination of the akinetes, which, until this paper, served as the diagnostic feature of the last. A complete summary of these genera illustrates the difficulty of using morphology alone to define them (Table 1).</p><p>...continued on the next page</p><p>Reference: (a) Xiao Y, Zhang X, Ji P (2015)</p><p>ND= no data</p><p>Molecular Characterization. The first tree, comprising 363 Operational Taxonomic Units (OTUs) with 752 positions analyzed (not shown), was constructed to identify the phylogenetically most related genera to  Reofilinostoc . Within this tree, the order  Nostocales forms a monophyletic group, and  Reofilinostoc matlalcueyense is situated within a clade alongside  Minunostoc and  Desikacharya ( Nodulariaceae) (ML=100). This clade is sister to another clade containing various genera from both  Nodulariaceae and  Nostocaceae families, including  Nodularia,  Aliinostoc,  Pseudoaliinostoc,  Amazonocrinis,  Atlanticothrix,  Cyanocohniella,  Halotia,  Nostoc,  Desmonostoc,  Komarekiella,  Roholtiella,  Mojavia and  Violetonostoc .</p><p>The ML phylogeny of  Nodulariaceae /  Nostocaceae (second stage analysis) (Fig. 3) was constructed using 101 OTUs and analyzing 1071 positions. At the base of the tree, the Pseudanabaenales are positioned, followed by the Chroococcidiopsidales clade, the sister group to  Nostocales . The  Nostocales group is monophyletic (ML=99), and all the genera exhibit strong bootstrap support. However, the relationships among these genera must be better established, as indicated by the low bootstrap values within the  Nostocales clade. This effect is likely attributed to the high similarity (p-distance) values shared among the  Nostocales genera, as further detailed in this paper.</p><p>In this tree, it is possible to divide the  Nostocales cluster into three major lineages. At the top of the tree, there’s the cluster containing the core of  Nodulariaceae, including the reference strain  Nodularia BCNOD 9427, along with other  Nodulariaceae genera like  Aliinostoc,  Pseudoaliinostoc,  Purpureonostoc,  Cyanocohniella,  Chrysosporum,  Anabaenopsis,  Goleter,  Halotia and  Atlanticothrix . Forming another lineage sister to this cluster, but with strong bootstrap support (ML=100), there is a lineage ( Nodulariaceae II), with two genera  Minunostoc and  Desikacharya .  Reofilinostoc is placed within this lineage, in it is clade, and is not closely related to any previously described genus. Furthermore, in proximity to  Reofilinostoc, there are other strains misidentified as  Nostoc, which must be described as new genera in the future. At the base of these two lineages, two strains are misidentified as “  Amazonocrinis ” alongside the  Nostocaceae genera  Komarekiella,  Violetonostoc,  Compactonostoc and  Dendronalium .</p><p>The third lineage is composed of the core  Nostocaceae, including the reference strain  Nostoc WY 1KK1, along with other  Nostoc species and  Desmonostoc . The type strain  Amazonocrinis nigriterrae CENA 67 is in a basal position in relation to the  Nostocaceae /  Nodulariaceae clade, rendering the genus a polyphyletic status.</p><p>According to this phylogeny,  Reofilinostoc was classified within the  Nodulariaceae family due to it is close phylogenetic relationship with  Minunostoc and  Desikacharya and with the core  Nodulariaceae clade.</p><p>The BI (Fig. 4) (Average Standard Deviation of Split Frequencies &lt;0,01) and ML (Fig. 5)  Nodulariaceae phylogenies (third stage phylogenies), with 56 OTUs and 1284 positions, confirmed the previous results and presented phylogenetic solid support at the backbone. Both trees exhibited identical topologies, consistent with the structure observed in the  Nodulariaceae /  Nostocaceae tree (second stage). Again, the  Nodulariaceae core is distinctly separated from the  Desikacharya /  Minunostoc cluster, where  Reofilinostoc is positioned. This separation of  Nodulariaceae in two lineages is strongly supported by BI posterior probabilities (BI=1), which this is also true in the ML tree. Moreover, the genera-level clades presented strong phylogenetic support in both phylogenies.</p><p>The phylogenetic relations among genera presented strong statistic support in the BI tree (Fig. 4) but not in the ML tree (Fig. 5), which is  Nodulariaceae /  Nostocaceae tree constructed. This is probably because of the high similarity (p-distance) values among genera, typical for  Nostocaceae and  Nodulariaceae .</p><p>BI and ML phylogenies confirm  Reofilinostoc as a new genus in it is clade among strains misidentified as  Nostoc . It cannot include these “  Nostoc ” strains in  Reofilinostoc because of their weak phylogenetic relation, evidenced primarily in the ML tree. The closest related genera to new genus are  Minunostoc and  Desikacharya .</p><p>The intrageneric similarity of the 16S rRNA gene (p-distance) analysis within  Nodulariaceae genera is presented in Table 2. It ranged from 97.2% within  Aliinostoc, to 100% within  Purpureonostoc and  Desikacharya . When comparing different genera, the minimum similarity value was 91%, observed between  Desikacharya and  Halotia . The highest similarity value comparing genera was 97.8%, found in  Halotia and  Goleter . This value overlapped the lowest intrageneric similarity observed (97.2%). Furthermore, this analysis revealed that some comparisons among  Nodulariaceae genera exhibited similarity values exceeding 97%, such as  Atlanticothrix vs.  Goleter and  Cyanocohniella vs.  Anabaenopsis . Based on these findings, the results of p-distance analysis also support the proposal of  Reofilinostoc, considering that it displayed a maximum similarity of 96.4%, observed when compared to  Desikacharya, which is a clearly separated clade. This value (96.4%) is lower than those cited above for other genera comparisons.</p><p>The 16S-23S rRNA ITS secondary structures analysis corroborates with the separation of  Reofilinostoc in a new cyanobacterial genus (Figs. 6, 7, 8). It was compared the D1-D1’, Box B and V3 helix of  Reofilinostoc with the closest phylogenetic-related genera, such as  Desikacharya nostocoides and  Minunostoc cylindricum . Because of the lack of both tRNAs Ile and  Ala in the sequence analyzed, it was not possible to retrieve the V2 helix from the  Reofilinostoc 16S-23S ITS region (Table 3).</p><p>The D1-D1’ helix of  Reofilinostoc (Fig. 6) exhibits a distinct nucleotide sequence and structure compared to  Desikacharya nostocoides . Both helices initially share a basal stem, commencing with 5’ GACCTACCCA 3’. However, beyond this point, the sequences of the helix diverge completely. Furthermore,  Desikacharya features two residues (5’ AC 3’) located opposite the first lateral bulge, while no residues occupy this position in  Reofilinostoc . Additionally,  D. nostocoides possesses two loops below the terminal loop, whereas  Reofilinostoc only has one. Although both genera have terminal loops with five residues, they differ significantly in sequence. The D1-D1’ helix of  Minunostoc cylindricum is remarkably different from the others, being much longer and presenting additional stems in the first lateral bulge region.</p><p>The Box B helices of  Reofilinostoc and  Desikacharya (Fig. 7) present the same basal stem, starting with the typical Box B initial sequence 5’AGCA3’, but after that they are very different in sequence and structure. In  Desikacharya the helix present three residues forming a central loop, while in  Reofilinostoc the structure present only one mismatch in this position. The terminal loops are different considering length and sequence. For  Minunostoc cylindricum, the helix is remarkably different in sequence, length and by presenting two loops below the terminal loop.</p><p>The V3 helices (Fig. 8) were the most variable among those genera. From  Desikacharya nostocoides is remarkably different, being much longer and presenting four loops below the terminal loop. While,  Reofilinostoc matlalcueyense F02 has a basal stem with a 13 bp helix followed by terminal loop consisted of four residues (AGAG). Also, the  Reofilinostoc differs from  Minunostoc cylindricum in length and structure.</p></div>	https://treatment.plazi.org/id/2B2B8798AC70D937DCAABBFFFC27F9A3	Public Domain	No known copyright restrictions apply. See Agosti, D., Egloff, W., 2009. Taxonomic information exchange and copyright: the Plazi approach. BMC Research Notes 2009, 2:53 for further explanation.		Plazi	Garduño-Solórzano, Gloria;Martínez-García, Martha;Hentschke, Guilherme Scotta;Solorza, Luis Fernando Miguel;Vasconcelos, Vitor M.	Garduño-Solórzano, Gloria, Martínez-García, Martha, Hentschke, Guilherme Scotta, Solorza, Luis Fernando Miguel, Vasconcelos, Vitor M. (2024): A phylogenetically distant clade of Nostoc-like (Cyanobacteria) taxa with the description of Reofilinostoc matlalcueyense gen. et sp. nov. from an extreme environment. Phytotaxa 655 (2): 125-143, DOI: 10.11646/phytotaxa.655.2.2, URL: http://dx.doi.org/10.11646/phytotaxa.655.2.2
