identifier	taxonID	type	CVterm	format	language	title	description	additionalInformationURL	UsageTerms	rights	Owner	contributor	creator	bibliographicCitation
039287F5F300D52EF389F963FCDC7BF3.text	039287F5F300D52EF389F963FCDC7BF3.taxon	http://purl.org/dc/dcmitype/Text	http://rs.tdwg.org/ontology/voc/SPMInfoItems#GeneralDescription	text/html	en	Lecanicillium gracile V. B. Ponizovskaya, A. A. Grum-Grzhim., Georgieva & Bilanenko 2020	<html xmlns:mods="http://www.loc.gov/mods/v3">
    <body>
        <div>
            <p> Lecanicillium gracile V.B. Ponizovskaya, A.A. Grum-Grzhim., Georgieva &amp; Bilanenko ,  sp. nov.</p>
            <p>MycoBank MB830764</p>
            <p>Figs. 2–3</p>
            <p>Etymology:— Latin, gracile = slender or thin; referring to slim phialides and thin mycelium.</p>
            <p>Type:— RUSSIA. Vladimir region: Kideksha village, Church of Boris and Gleb (XII century), wall area with high moisture content and precipitation of salts at the surface, loose limestone, 13/June/2013, collected by V.B. Ponizovskaya, N.L. Rebrikova, isolated by V.B. Ponizovskaya (holotype CBS H-23932), ex-type culture Kd1 (CBS 142821).</p>
            <p>......continued on the next page</p>
            <p>Culture characteristics: Colonies on PDA reaching 14–30 mm diam. in 10 days and 20–39 mm diam. in 14 days. Colonies white, cottony, centrally raised, colony surface or at least marginal parts more or less radially wrinkled, margin entire, no exudate and soluble pigments, reverse yellowish to creamish. Colonies on PCA reaching 19–24 mm diam. in 10 days and 26–31 mm diam. in 14 days. Colonies white, floccose, sometimes waxy near margin, more or less centrally raised, margin entire, submerged, no exudate and soluble pigments, reverse creamish. Colonies on MYA reaching 21–26 mm diam. in 10 days and 27–33 mm diam. in 14 days. Colonies white, cottony, more or less centrally raised, colony surface or marginal parts more or less radially wrinkled, margin entire, no exudate and soluble pigments, reverse creamish to brownish.</p>
            <p>Phialides (6–)10–23(–33) μm long (mean ± standard deviation 15.7±5.3), basal part (0.5–)0.8–1.5 (1±0.2) μm wide, tip 0.2–0.9 μm wide (0.5±0.1), slender, tapering toward the tip, produced solitary or less often in whorls of 2–3 (rarely 4–5) on prostrate conidiophores; sometimes also produced on short erect conidiophores or secondarily formed on the preceeding phialides. Conidia formed in heads in the apex of the phialides, dimorphic, macroconidia mostly with pointed ends, fusiform to slightly falcate, smooth-walled, 1-celled, 3.6–8.0 × 1.7–0.8 μm (5.4±0.9 × 1.2±0.2), microconidia ovate, obovate, ellipsoidal, sometimes fusiform and slightly curved, smooth-walled, 1-celled, 1.1–3.3 × 0.7–1.4 μm (2.3±0.45 × 1.1±0.2). No microscopic crystals observed.</p>
            <p>Sexual stage not observed.</p>
            <p>Temperature optimum for growth of Kd1 (CBS 142821) is 20–23 °C (19–33 mm diam. in 14 d), reduced growth at 28 °C (5–7 mm diam. in 14 d), no growth occurring at 30 °C (Fig. 4).</p>
            <p>Temperature optimum for growth of 57-9-2 (CBS 142816) is 23–25 °C (30–38 mm diam. in 14 d), reduced growth at 33 °C (3–7 mm diam. in 14 d), no growth occurring at 35 °C (Fig. 4).</p>
            <p>Temperature optimum for growth of G4-5 (VKPM F-1421) is 23–25 °C (26–39 mm diam. in 14 d), reduced growth at 33 °C (2–3 mm diam. in 14 d), no growth occurring at 35 °C (Fig. 4).</p>
            <p>ITS differences with the closest taxa</p>
            <p> Lecanicillium gracile (MF682448, Kd1, type) differs from  L. restrictum (LT548279, CCF 5252, type) by 22 SNPs, two 1-bp deletions, three 1-bp insertions in a total of 1034 character alignment (97.4 % similarity).  Lecanicillium gracile (MF682448, Kd1, type) differs from  L. testudineum (LT548278, CCF 5201, type) by 24 SNPs, one 1-bp deletion, three 1-bp insertions in a total of 1033 character alignment (97.3 % similarity).  Lecanicillium gracile (MF682448, Kd1, type) differs from  L. coprophilum (MH177616, CGMCC 3.18986, type) by 22 SNPs, three 1-bp deletions, one 1-bp insertion in a total of 541 character alignment (95.2 % similarity).  Lecanicillium gracile (MF682448, Kd1, type) differs from  P. album (LC092880, CBS 504.83, type) by 15 SNPs, seven deletions (five 1-bp deletions and two 4-bp deletions), one 1-bp insertion in a total of 548 character alignment (94.7 % similarity). </p>
            <p>TEF1a differences with the closest taxa</p>
            <p> Lecanicillium gracile (MN602801, Kd1, type) differs from  L. restrictum (LT626943, CCF 5252, type) by 33 SNPs in a total of 459 character alignment (92.8 % similarity).  Lecanicillium gracile (MN602801, Kd1, type) differs from  L. testudineum (LT626942, CCF 5201, type) by 27 SNPs in a total of 459 character alignment (94.1 % similarity).  Lecanicillium gracile (MN602801, Kd1, type) differs from  L. coprophilum (MH184587, CGMCC 3.18986, type) by 26 SNPs in a total of 412 character alignment (93.7 % similarity). </p>
        </div>
    </body>
</html>
	https://treatment.plazi.org/id/039287F5F300D52EF389F963FCDC7BF3	Public Domain	No known copyright restrictions apply. See Agosti, D., Egloff, W., 2009. Taxonomic information exchange and copyright: the Plazi approach. BMC Research Notes 2009, 2:53 for further explanation.		Plazi	Ponizovskaya, Valeria B.;Grum-Grzhimaylo, Alexey A.;Georgieva, Marina L.;Kokaeva, Lyudmila Y.;Bilanenko, Elena N.	Ponizovskaya, Valeria B., Grum-Grzhimaylo, Alexey A., Georgieva, Marina L., Kokaeva, Lyudmila Y., Bilanenko, Elena N. (2020): Lecanicillium gracile (Cordycipitaceae), a new species isolated from mineral building materials. Phytotaxa 443 (3): 265-278, DOI: 10.11646/phytotaxa.443.3.3, URL: http://dx.doi.org/10.11646/phytotaxa.443.3.3
