identifier	taxonID	type	CVterm	format	language	title	description	additionalInformationURL	UsageTerms	rights	Owner	contributor	creator	bibliographicCitation
03957532FC2EDE0CFF1784D25646F97A.text	03957532FC2EDE0CFF1784D25646F97A.taxon	http://purl.org/dc/dcmitype/Text	http://rs.tdwg.org/ontology/voc/SPMInfoItems#GeneralDescription	text/html	en	Amphisphaeria guizhouensis Q. Yang, Q. R. Li & Cheewangkoon 2025	<div><p>Amphisphaeria guizhouensis Q. Yang, Q.R. Li &amp; Cheewangkoon, sp. nov. (Fig. 2).</p><p>MycoBank number: MB859927</p><p>Etymology: —The specific epithet “ guizhouensis ” refers to the type location, Guizhou Province, China.</p><p>Description: Saprobic on dead branches of an unidentified woody plant. Sexual morph: Ascomata 265–341 × 405–547 µm (x = 295 × 484 µm, n = 5), black, scattered, with a cracked surface, immersed beneath the host epidermis, spherical to subspherical; Ostiole papillate, 129–164 µm wide (x =142 µm, n = 5), yellowish-brown centric. Peridium 26–42 µm thick, composed of layers of cortical cells, the inner layer is reddish-brown and triangular, while the outer layer consists of brownish-black to black cells of textura angularis. Paraphyses 3.7–5.5 µm wide (x = 4.4 µm, n = 20), hyaline, cellular, septate with constrictions, guttulate. Asci 142–168 × 7–9.8 µm (x = 156 × 8 µm, n = 30), 8-spored, unitunicate, cylindrical, with a short cylindrical pedicel and a distinct apical ring, J- in Melzer’s reagent. Ascospores 19–23.8 × 4.9–6.3 µm (x = 20.9 × 5.7 µm, n = 30), L/W ratio 3.6, uniseriate, ellipsoidal to fusiform, guttulate, hyaline when immature, becoming pale brown at maturity, 3-septate, with round ends. Asexual morph: Undetermined.</p><p>Culture characteristics: Colony on PDA is irregular, reaching 40 mm in diameter after 14 days at 28°C in a constant-temperature incubator. The surface is smooth, with dense, fluffy, compact, and dry mycelia. In the lateral view, the colony is raised with a well-defined edge. From above, the colony is white to cream-colored. From below, the edge is pale yellow, the center is orange-yellow, and the core is brown. Yellow pigments are produced during cultivation.</p><p>Specimen examined: CHINA, Guizhou Province, Tongren City, <a href="https://tb.plazi.org/GgServer/search?materialsCitation.longitude=108.01&amp;materialsCitation.latitude=27.292555" title="Search Plazi for locations around (long 108.01/lat 27.292555)">Foding Mountain Forest Nature Reserve</a>, 27°17′33.20″N, 108°0′36.02″E, elevation 1043 m, on dead branches of an unidentified woody plant, 7 August 2024, Qing Yang, 2024FDSYJZ77 (GMB5901 Holotype), ex-type living culture GMBC5901 ; ibid., (KUN-HKAS 146995, Isotype) .</p><p>Additional material examined (paratype): CHINA, Guizhou Province, Tongren City, <a href="https://tb.plazi.org/GgServer/search?materialsCitation.longitude=108.144615&amp;materialsCitation.latitude=27.327028" title="Search Plazi for locations around (long 108.144615/lat 27.327028)">Foding Mountain Forest Nature Reserve</a>, 27°19′37.30″N, 108°8′40.62″E, elevation 650 m, on dead branch of unidentified woody plant, 8 August 2024, Qing Yang, 2024FDSDCY72 (paratype GMB5902, exparatype GMBC5902) .</p><p>Notes: Amphisphaeria guizhouensis is placed within the genus Amphisphaeria based on the present phylogenetic analyses and morphology (Figure 1). Multi-loci phylogeny shows that A. guizhouensis forms a sister clade with A. curvaticonidia with 98% ML and 0.99 BYPP support value (Figure 1). In BLAST searches, the ITS and LSU sequence of A. guizhouensis shows 93.6% and 95.55% similarity to A. curvaticonidia . Moreover, the new species can be distinguished by its larger ascomata (265–341 × 405–547 µm vs. 320–390 × 360–410 µm), narrower asci (142–168 × 7–9.8 µm vs. 121–162 × 10.5–17.5 µm), and narrower ascospore (19–23.8 × 4.9–6.3 µm vs. 17–23 × 6–9 µm). The apical ring of asci in A. guizhouensis did not show a blue colour change with Melzer’s reagent, but A. curvaticonidia showed the color change to blue (Samarakoon et al. 2020). The ITS and LSU sequences of Amphisphaeria guizhouensis showed 93.68% and 96.26% similarity to Lepteutypa qujingensis (HMAS 290478). Morphologically, A. guizhouensis can be distinguished from L. qujingensis by its thicker peridium (26–42 vs. 10–25 µm), narrower paraphyses (3.7–5.5 vs. 6.5–12 µm), and larger asci (142–168 × 7–9.8 µm vs. 100–140 × 4.5–8.5 µm). In addition, A. guizhouensis is J- in Melzer’s reagent, whereas L. qujingensis is J+ (Dissanayake et al. 2020). The new species also differs from A. parvispora by having larger asci (142–168 × 7–9.8 µm vs. 62–105 × 5–6.5 μm) and ascospores (19–23.8 × 4.9–6.3 µm vs. 9.5–11.5 × 3–4 μm) (Samarakoon et al. 2022). Moreover, the asci of A. guizhouensis are larger than those of A. ailaoshanensis (142–168 × 7–9.8 µm vs. 70–100 × 7–10 μm) (Dissanayake et al. 2024).</p></div>	https://treatment.plazi.org/id/03957532FC2EDE0CFF1784D25646F97A	Public Domain	No known copyright restrictions apply. See Agosti, D., Egloff, W., 2009. Taxonomic information exchange and copyright: the Plazi approach. BMC Research Notes 2009, 2:53 for further explanation.		Plazi	Yang, Qing;Ren, Yu-Lin;Liu, Li-Li;Lu, Chang-Tao;Wijayawardene, Nalin N.;Haituk, Sukanya;Li, Qi-Rui;Cheewangkoon, Ratchadawan	Yang, Qing, Ren, Yu-Lin, Liu, Li-Li, Lu, Chang-Tao, Wijayawardene, Nalin N., Haituk, Sukanya, Li, Qi-Rui, Cheewangkoon, Ratchadawan (2025): Amphisphaeria guizhouensis sp. nov. (Amphisphaeriaceae, Amphisphaeriales) from Guizhou Province, China. Phytotaxa 721 (3): 218-228, DOI: 10.11646/phytotaxa.721.3.2, URL: https://doi.org/10.11646/phytotaxa.721.3.2
