identifier	taxonID	type	CVterm	format	language	title	description	additionalInformationURL	UsageTerms	rights	Owner	contributor	creator	bibliographicCitation
03F087A4FFC506309C6AFCFD7759F9F7.text	03F087A4FFC506309C6AFCFD7759F9F7.taxon	http://purl.org/dc/dcmitype/Text	http://rs.tdwg.org/ontology/voc/SPMInfoItems#GeneralDescription	text/html	en	Elisa	<html xmlns:mods="http://www.loc.gov/mods/v3">
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            <p> ELISA laboratory methods </p>
            <p> NVN, UTAD, UAB and HUJ each performed an  ELISA , either an in-house or a commercial  ELISA (NVN and UTAD) .  ELISA was performed in accordance with each laboratory’s specifications [9, 13, 33]. UTAD  ELISA was performed in accordance with the Leiscan  Leishmania ELISA Test protocols [33]. NVN  ELISA was conducted in accordance with the MegaELISA LEISH (MEGA- COR Veterinary Diagnostics, Hörbranz, Austria) test protocols [34]. In summary,  Leishmania antigen was prepared using total  L. infantum antigen obtained from 3 × freeze-thawed stationary phase  L. infantum promastigotes (strains utilized are unique to each laboratory) [9, 13]. Microtitre plates were coated with  L. infantum antigen; antigen source and concentration varied by laboratory and in-house protocol [9, 13]. Plates were incubated overnight at between 2 and 8 ℃ dependent on the laboratory protocol [9, 13]. Canine serum was tested at different dilutions: 1:20 for UTAD, 1:800 for UAB, 1:100 for NVN and 1:1000 for HUJ. For HUJ and UAB protocols, plates were resolved via protein A conjugated to horseradish peroxidase for 1 h at 37 ℃ [9, 13]. Each plate was read when the positive canine reference serum’s absorbance (405 nm or 492 nm) reached a pre-determined cut-off value [9, 13]. Results were then determined based on each laboratory’s cut-off value: 0.4 for NVN, 0.8 for UTAD, 0.28 for UAB and 0.2 for HUJ [9, 13, 33]. </p>
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	https://treatment.plazi.org/id/03F087A4FFC506309C6AFCFD7759F9F7	Public Domain	No known copyright restrictions apply. See Agosti, D., Egloff, W., 2009. Taxonomic information exchange and copyright: the Plazi approach. BMC Research Notes 2009, 2:53 for further explanation.		Plazi	Mahachi, Kurayi G.;Ozanne, Marie;Bourdeau, Patrick;Sarquis, Juliana;Kontowicz, Eric;Solano-Gallego, Laia;Cardoso, Luis;Oliva, Gaetano;Baneth, Gad;Pennisi, Maria Grazia;Toepp, Angela M.;Miró, Guadalupe;Carrel, Margaret;Petersen, Christine A.	Mahachi, Kurayi G., Ozanne, Marie, Bourdeau, Patrick, Sarquis, Juliana, Kontowicz, Eric, Solano-Gallego, Laia, Cardoso, Luis, Oliva, Gaetano, Baneth, Gad, Pennisi, Maria Grazia, Toepp, Angela M., Miró, Guadalupe, Carrel, Margaret, Petersen, Christine A. (2024): Comparison of ELISA and IFAT for LEiSHMania infanTUM by European and Middle Eastern diagnostic laboratories. Parasites & Vectors (545) 17 (1): 1-11, DOI: 10.1186/s13071-024-06631-9, URL: https://doi.org/10.1186/s13071-024-06631-9
