Cetrelia cetrarioides (Delise) W.L. Culb. & C.F. Culb.

Farkas, Edit, Biró, Bernadett, Varga, Nóra, Sinigla, Mónika & Lőkös, László, 2021, Analysis of lichen secondary chemistry doubled the number of Cetrelia W. L. Culb. & C. F. Culb. species (Parmeliaceae, lichenised Ascomycota) in Hungary, Cryptogamie, Mycologie 20 (1), pp. 1-16 : 5

Cetrelia cetrarioides (Delise) W.L. Culb. & C.F. Culb.

( Figs 1C ; 2 ; 5A ; 6 ; 7 ; 12 )

It is characterised by the presence of atranorin, perlatolic acid (major), ± imbricaric acid (minor) and anziaic acid. The lack of 4-O-demethylimbricaric acid is obvious and well observable already under UV 254 nm after developing the HPTLC plate ( Fig. 2 ). Its soredia are fine [25-35(-40) µm in diam. – Obermayer & Mayrhofer (2007)], 32.3 ± 3.4 µm in Hungarian samples. Not or slightly raised pseudocyphel - lae of various size (c. 30-300 µm) occur on upper cortex, smaller ones on lower cortex. Its specimens were found most frequently on oak ( Quercus sp. – 38%), beech ( Fagus sylvatica L. – 15%) and other tree species (46%) at lower elevations than in Austria ( Obermayer & Mayrhofer 2007), mostly at 200-600 m a.s.l., though in Belarus it occurs at even lower elevation ( Bely et al. 2014). This species proved to be less frequent than expected ( Figs 6 ; 7 ), and was previously confused with C. monachorum . Cetrelia cetrarioides is considered here as a critically endangered (CR) species in Hungary.

Figures

FIG. 1. — Differential morphological and chemical features in Cetrelia W.L. Culb. & C.F. Culb. species: A, large, not raised pseudocyphellae on upper cortex of C. chicitae (W.L. Culb.) W.L. Culb. & C.F. Culb.; B, small, raised pseudocyphellae on upper cortex of C. monachorum (Zahlbr.) W.L. Culb. & C.F. Culb.; C, brown lower cortex of C. cetrarioides (Delise) W.L. Culb. & C.F. Culb. without rhizines; D, C+ reaction by NaOCl on marginal soralia of Cetrelia olivetorum (Nyl.) W.L. Culb. & C.F. Culb. Scale bars: 500 µm.

FIG. 2. — A detail of the chromatographic plate HPTLC nr 74/2014 developed in solvent system C presenting all species under UV 254 nm (A), and sprayed with water (B). Specimens in positions A10-A17 are A10: C. olivetorum (Nyl.) W.L. Culb. & C.F. Culb. (BP 21529), A11: C. monachorum (Zahlbr.) W.L. Culb.& C.F. Culb. (BP 85318),A12: C. olivetorum (BP 84893), A13: C. cetrarioides (Delise) W.L. Culb. & C.F. Culb. (BP 21538),A14: C. olivetorum (BP 22787), A15: C. monachorum (BP 45013), A16: C. olivetorum (BP 21508), A17: C. chicitae (W.L. Culb.) W.L. Culb. & C.F. Culb. (BP 93416). Abbreviations of LSMs are according to Table 2.

FIG. 5. — Thalline lobes with marginal soralia of A, Cetrelia cetrarioides (Delise) W.L. Culb. & C.F. Culb.; B, C. chicitae (W.L. Culb.) W.L. Culb. & C.F. Culb.; C, C. monachorum (Zahlbr.) W.L. Culb. & C.F. Culb.; and D, C. olivetorum (Nyl.) W.L. Culb. & C.F. Culb. Scale bars: 1 mm.

FIG. 6. — Distribution of Cetrelia cetrarioides(Delise) W.L.Culb.& C.F.Culb.in Hungary before revision.

FIG. 7. — Distribution of Cetrelia cetrarioides(Delise) W.L.Culb.& C.F.Culb. in Hungary after revision.

FIG. 12. — Altitudinal distribution of Cetrelia W.L.Culb.& C.F.Culb.species in Hungary.