Autochton (Autochton) dora Grishin, 2023

Zhang, Jing, Cong, Qian & Grishin, Nick V., 2023, Supplementary Materials and Appendix, Insecta Mundi 2023 (26), pp. 1-115 : 13-14

Autochton (Autochton) dora Grishin , new species

https://zoobank.org/ 2CBF81CD-A45A-40E8-B6B9-E4E64513578D

( Fig. 1 part, 23–24, 235–236)

Definition and diagnosis. Ecuadorian specimens identified (incorrectly) as Autochton neis (Geyer, 1832) (type locality in Brazil) differ prominently from it in Z chromosome genes ( Fig. 1a) and, therefore, although not differing in the COI barcode (0.15%, 1 bp), represent a new species. This new species partly keys to “ Autochton neis ” (C.16.6) in Evans (1952) and has somewhat similar male genitalia but differs from A. neis and other species of Autochton in a rounder distal end of harpe (near ventral margin), harpe with terminally smaller, rounded dorsal projection ( Fig. 235–236), two (not three) subapical forewing spots, less irregular forewing hyaline discal band with spots more aligned with each other, and the spot in M 3 -CuA 1 cell being minute, dot-like ( Fig. 23–24). Due to the cryptic nature of this species, most reliable identification is achieved by DNA and a combination of the following base pairs is diagnostic in the nuclear genome: aly159.20.1:G156A, aly159.20.1:C183A, aly214.14.1:G229T, aly525.55.2:G403T, aly275215.9.3:A42C, however COI barcodes do not differentiate this species from A. neis .

Barcode sequence of the holotype. Sample NVG-22032C11, GenBank OR837631, 658 base pairs: AACTTTATATTTTATTTTTGGAATCTGAGCAGGATTAGTAGGTACTTCTTTAAGATTACTAATTCGAACTGAACTAGGAACACCTGGATCATTAATT GGAGATGATCAAATTTATAATACTATTGTTACTGCTCATGCTTTTATTATAATTTTTTTTATAGTTATACCTATTATAATTGGAGGATTTGGAAACT GACTAGTACCCCTTATACTAGGAGCTCCAGATATAGCCTTCCCACGAATAAATAATATAAGATTCTGACTATTACCTCCATCATTAACTCTTTTAAT TTCAAGTAGTATTGTAGAAAATGGAGCAGGAACTGGATGAACTGTTTATCCTCCCCTTTCTTCTAATATTGCTCATCAAGGAGCTTCAGTAGATTTA GCAATTTTTTCTCTTCATTTAGCAGGAATTTCTTCAATTTTAGGAGCTATTAATTTTATTACAACTATTATTAATATACGAATTAATAATATATCTT TTGATCAAATACCTTTATTTGTATGAGCTGTAGGAATTACAGCTCTTCTTCTTTTACTTTCTTTACCAGTTCTAGCTGGAGCTATTACTATATTATT AACAGATCGAAATTTAAATACTTCATTCTTTGACCCAGCTGGGGGAGGAGATCCTATTTTATATCAACATTTATTT

Type material. Holotype: ♂ deposited in the National Museum of Natural History, Smithsonian Institution , Washington, DC, USA ( USNM), illustrated in Fig. 23–24, bears the following three rectangular labels, two white: [ ECUADOR Pastaza | Puyo-Napo Rd. | Km 25 - 1200m | 11 Nov. ’88 | S.S. Nicolay], [DNA sample ID: | NVG-22032C11 | c/o Nick V. Grishin], and one red [HOLOTYPE ♂ | Autochton (Autochton) | dora Grishin ] . Paratype: 1♀ NVG-19119C04, USNMENT_01602625 the same data as the holotype but collected on 10-Sep-1977.

Type locality. Ecuador: Pastaza Province, km 25 of Puyo-Napo road, elevation 1200 m.

Etymology. The name is for its distribution in [Ecua] dor + a and is a noun in apposition.

Distribution. Known only from the type locality in Ecuador.