Thespieus mandal Grishin, 2023

Zhang, Jing, Cong, Qian & Grishin, Nick V., 2023, Supplementary Materials and Appendix, Insecta Mundi 2023 (26), pp. 1-115 : 45-46

Thespieus mandal Grishin , new species

https://zoobank.org/ BA7D773F-A815-4251-B153-DC7B3CAC5B13

( Fig. 4 part, 103–104, 328–329)

Definition and diagnosis. Phylogenetic analysis of a specimen from Rio de Janeiro, Brazil, identified as Thespieus dalman (Latreille, [1824]) (type locality in Brazil, lectotype sequenced as NVG-18078F01) reveals its strong genetic differentiation ( Fig. 4): e.g., COI barcode difference of 3.8% (25 bp) from T. dalman , and therefore it represents a new species. This new species keys to T. dalman (O.7.4) in Evans (1955) but differs from it by a generally narrower hyaline spot in the forewing discal cell but wider spot in the hindwing cell CuA 1 -CuA 2, which is framed by a smaller and paler brown spot at the base of this cell on the ventral side, which has no brown discal dash in cell Sc+R 1 -RS, and exhibits paler basal area in cell 1A+2A-3A ( Fig. 103–104), harpe wider, protrudes dorsad farther than ampulla, more concave along the distal margin, saccus stronger bowed ventrad ( Fig. 328–329). Due to the cryptic nature of this species, most reliable identification is achieved by DNA and a combination of the following base pairs is diagnostic in the nuclear genome: aly168.8.1:G313A, aly 2517.1.2:T285C, aly3905.3.3:A99C, aly971.11.1:A75G, aly3268.8.1:T840C, aly10226.17.4:A204A (not C), aly320.37.1:G189G (not A), aly1656.14.1:C411C (not T), aly594.20.16:C67C (not A), aly 1651.3.5:A72A (not T), and COI barcode: T50C, T133C, T340C, T352C, A379C, T640C.

Barcode sequence of the holotype. Sample NVG-18012A11, GenBank OR837670, 658 base pairs: AACTTTATATTTTATTTTTGGTATTTGAGCAGGAATATTAGGAACTTCACTAAGATTACTAATTCGTACAGAATTAGGTAATCCAGGATCTTTAATT GGAGATGATCAAATTTATAATACTATTGTTACAGCCCATGCTTTTATTATAATTTTTTTTATAGTTATACCTATTATAATTGGAGGATTTGGAAATT GATTAGTTCCATTAATATTAGGAGCTCCTGATATAGCTTTTCCTCGAATAAATAATATAAGATTTTGAATATTACCTCCCTCTTTAACATTATTAAT TTCAAGAAGAATTGTAGAAAATGGTGCAGGAACTGGATGAACAGTTTACCCCCCCTTATCCTCTAATATTGCTCATCAAGGATCTTCCGTAGATTTA GCAATTTTCTCACTTCATTTAGCTGGAATTTCATCTATTTTAGGAGCTATTAATTTTATTACAACAATTATTAATATACGAATTAAAAATTTATCAT TTGATCAAATACCTTTATTTGTATGATCTGTAGGTATTACAGCTTTATTATTACTTTTATCTTTACCTGTATTAGCTGGTGCTATTACCATATTATT AACAGATCGAAATTTAAATACTTCATTCTTTGACCCTGCAGGAGGAGGAGATCCAATCTTATATCAACATTTATTT

Type material. Holotype: ♂ currently currently deposited in the National Museum of Natural History, Smithsonian Institution, Washington, DC, USA ( USNM), illustrated in Fig. 103–104, bears the following six rectangular labels, five white: [ BRAZIL, RJ, P. N. de | Itatiaia, 800m | 22°27′S, 44°37′W | 7Apr 1995 | Diversity Project UERJ], [ Thespieus | dalman], [no transect], [DNA sample ID: | NVG-18012A11 | c/o Nick V. Grishin], [USNMENT | {QR Code} | 01450264], and one red [HOLOTYPE ♂ | Thespieus | mandal Grishin ].

Type locality. Brazil: Rio de Janeiro, Itatiaia National Park, elevation 800 m, GPS −22.450, −44.617.

Etymology. The name reverses syllables in its sister species, T. dalman . The name is a noun in apposition.

Distribution. Southeast Brazil.