Dermanyssus gallinae (Entrekin & Oliver, 1982)

Qi, Xiaoxiao, Li, Huan, Liu, Xin, Wang, Bohan, Meng, Jiali, Liu, Qi, Sun, Weiwei & Pan, Baoliang, 2023, Location of olfactory organs and architecture of gustatory organs in the poultry red mite, Dermanyssus gallinae (Acari: Dermanyssidae), Zoologischer Anzeiger 303, pp. 1-9 : 3

2.3. Manipulation of D. gallinae

To confirm if the olfactory sensilla were located at the tips of the foreleg tarsi of mites, we varnished the tips of the foreleg tarsi of female mites (VTFM) with blue nail polish ( Fig. 1D ) to prevent them from perceiving volatiles from fecal extracts. The responses of VTFM to the attractant pheromone and the potential arrestment pheromone (guanine, Aladdin) were compared with those of unvarnished female mites (UM) by Y-tube olfactometer bioassays and filter paper contact bioassays, respectively. We varnished the back of the mites (VBM) with blue nail polish in control to exclude the potential negative impacts of nail polish on the mite.

Figures

Fig. 1. Content from the centrifuge tube of mite trap (A) The dead mites, eggs and feces of D. gallinae under a stereomicroscope (B) The dead mites, eggs of D. gallinae remained on the 100-mesh screen (C) Feces of D. gallinae passed through the mesh screen (D) The tips of the foreleg tarsi of D. gallinae varnished with nail polish.