Fungal

Sun, Bind-Da, Zhou, Yu-Guang & Chen, Amanda-Juan, 2017, Bisifusarium tonghuanum (Nectriaceae), a novel species of Fusarium-like fungi from two desert oasis plants, Phytotaxa 317 (2), pp. 123-129 : 123

Fungal isolation and preservation

Plant material of Amaranthaceae plants, Atriplex centralasiatica Iljin and Suaeda salsa (L.) Pall. were thoroughly washed in distilled water. Then surface sterilization was performed by the following immersion sequence: 75% ethanol for 1 min, NaOCl (3% available chlorine) for 3 min, 75% ethanol for 1 min. The sterilized plant materials were then washed twice in autoclaved distill water and dried on sterilized paper before cutting into small segments or slices of 5 mm in length. After that, the sterilized segments or slices were placed on 2% malt extract agar (MEA) supplemented with chloromycetin (100 mg /L) and Rose Benga (33 mg /L) and incubated in the dark at 25 oC. Fungal colonies that developed around the plant fragments were purified on MEA plates and then transferred to new MEA slants. The dried specimen was deposited in the Herbarium Mycologicum Academiae Sinicae (HMAS), and the ex-type living strain was preserved in the China General Microbiological Culture Collection Center (CGMCC).