Draconibacterium mangrovi, Hu & Guo & Lai & Dong & Huang, 2020

Hu, Yuzhong, Guo, Yu, Lai, Qiliang, Dong, Le & Huang, Zhaobin, 2020, DraCOnibaCTerium mangrOVi sp. nov., isolated from mangrove sediment, International Journal of Systematic and Evolutionary Microbiology 70 (8), pp. 4816-4821 : 4819-4820

DESCRIPTION OF DRACONIBACTERIUM MANGROVI SP. NOV.

Draconibacterium mangrovi (man.gro'vi. N.L. gen. n. mangrovi of a mangrove).

Colonies on MB agar plates are light yellow, small and round. Cells are Gram-stain-negative, facultatively anaerobic, slightly curved and long rod-shaped, 5–6.5 µm long and 0.6 µm wide, with no flagellum. Catalase activity is weak positive and oxidase activity is positive. Growth is observed at 15–40 °C (optimum, 35 °C), NaCl tolerance of 0–5% (w/v; optimum, 2%) and pH 5.0–9.0 (optimum, pH 7.0). It has no ability to degrade soluble starch, skimmed milk or carboxymethyl cellulose. Grows slowly on nitrogenfree medium.

This species shows positive results for alkaline phosphatase, esterase (C4), esterase lipase (C8), leucine arylamidase, valine arylamidase, cystine arylamidase, trypsin, α -chymotrypsin, acid phosphatase, naphthol-AS- BI-phosphohydrolase, α -galactosidase, β -galactosidase, α -glucosidase, β -glucosidase, N -acetyl- β -glucosaminidase and β -fucosidase; weak positive for lipase (C14), β -glucuronidase and α -mannosidase. Reduction of nitrate to nitrite is positive. Production of indole is weak positive. Hydrolysis of aesculin is positive. Positive for 4-nitropheny l- β -D-galactopyranoside. Hydrolysis of gelatin is negative. Fermentation of amygdalin is positive and fermentation of rhamnose, sucrose and melibiose is weakly positive.

The predominant menaquinone is MK-7. The major fatty acids are (>10%) iso-C 15:0, anteiso-C 15:0 and C 17:1 ω 6 c. The polar lipids include phosphatidylethanolamine, a phospholipid and several unidentified lipids. The draft genome size is 5.9 Mb with genomic G+C content of 40.8 mol%.

The type strain, GM2-18 T (=MCCC 1 K04382 T =KCTC 72879 T), was isolated from mangrove sediment sampled at Luoyang River estuary in Quanzhou bay, Fujian, PR China. The GenBank/EMBL/DDBJ accession number of the 16S rRNA gene sequence of strain GM2-18 T is MN908333 . The draft genome sequence has been deposited at GenBank under the accession number JAAAGB000000000 .