Syngamus trachea

Meister, Seraina L., Wenker, Christian, Wyss, Fabia, Zühlke, Irene, In, Veiga, es Berenguer & Basso, Walter U., 2022, Syngamus trachea in free-ranging white stork (Ciconia ciconia) nestlings in Switzerland, International Journal for Parasitology: Parasites and Wildlife 18, pp. 76-81 : 77

2.5.1. Syngamus trachea identification

DNA was extracted from a total of five female S. trachea worms (one worm per infected stork). Prior to the DNA extraction, the worms were washed using isotonic NaCl solution. A piece of approximately 3 mm length was excised from the female worms’ body and dried at room temperature for at least 2 h. The DNA was then extracted from the tissue using the Spin-Column Protocol for animal tissues from the DNeasy® Blood & Tissue Kit ( QIAGEN GmbH, Hilden , Germany) according to the manufacturer’ s protocol .