Bolua balikesirensis Uluar & Çıplak, 2022

Uluar, Onur, Yahyaoğlu, Özgül & Çiplak, Battal, 2022, Anatolian endemic genus Bolua (Orthoptera: Tettigoniidae: Tettigoniinae): genetic and phenotypic data indicate inconsistent diversity and evolutionary patterns, Zootaxa 5195 (3), pp. 224-240 : 232-233

Bolua balikesirensis Uluar & Çıplak sp. n.

( Figures 4, 7, 10, 13, 16 , 19, 22, 25 , 27 , 28 , 31 ; Tables 1–5 )

LSID urn:lsid:zoobank.org:act:CD84EF90-FE17-45C0-9121-F1902C616071

Material examined: Holotype male, TURKEY, Balıkesir, Edremit, Kaz Dağları National Park, Sarıkız Rd. , 39.68975°N, 26.90948°E, 1470m, 10.08.2010 (leg. B. Çıplak, S.Kaya & Z. Boztepe) ( MEVBIL) ; Paratypes 6 males, 2 females, same data as holotype .

Description and diagnosis: As mentioned for the previous new species, members of the genus are similar in general appearance. Thus, the generic description can be applied to all species for certain features of the head, thorax and abdomen ( Figures 4, 7, 10, 13, 16 , 19, 22, 25 , 27 , 28 , 31 ; Tables 1–5 ). To avoid repetition, a detailed description is not presented here.

The new species B. balikesirensis sp. n. well differs from the other two by typical male cercus (tooth is near to middle in the first while in the beginning of apical 1/ 3 in the second; compare Figure 9 with 8 and 10). The male calling song consists of regular echemes including 10–26 (mean 18) syllables is another prominent diagnostic characteristic of B. balikesirensis sp. n. ( Figure 28 ), distinguishing it from B. bursaensis sp. n. ( B. turkiyae is unknown for the song). In addition to morphology, the new species that corresponds to PG 2 in the phylogenetic tree ( Figure 2 ) can be well distinguished from the other two by 71 unique mutations ( Figure 31 ). The high net pairwise distances and F ST values with other phylogroups also support its distinctness as a species ( Table 3 ). This species seems to be restricted to the Kazdagları Mts in Balıkesir Province of Turkey ( Figure 1 ).

Etymology: The new species Bolua balikesirensis was recorded from Kazdağları Mts located in Balıkesir Province of Turkey.

Figures

FIGURE 1. Distribution of Bolua [for detail of the localities see material examined per species; Bolu(1), Kastamonu (2,3,4, 6), Karabük (5), Çankırı (7), Balıkesir (8), Bursa (9, 10), Kütahya (11) and Kocaeli (12, 13)]

FIGURE 2. Phylogenetic tree, chronogram and taxonomic pattern obtained from the matrix constituted by concatenation of COI+NAD2+VAL+ITS sequences. The bootstrap (ML)/ posterior probability (BI) supports to the node are given above the node and the time to the most recent common ancestor (TMRCA) and the 95% HPD as million years below the node. The results from the species delimitation test are indicated by a bar line.

FIGURE 27. The PCA plots and trait contribution prepared from the metric data sets of male and female individuals belonging to Bolua

FIGURE 31. The ML tree showing unique base changes per phylogroups of Bolua, obtained by maximum parsimony analysis (the base position indicates by the position number in the concatenated matrix)

FIGURES 3–17. 3–5. Male head+pronotum+tegmina in Bolua (3—B. turkiyae, 4—B. balıkesirensis, 5—B. bursaensis; D— dorsal view, L—lateral view). 6–8. Male anal tergite in Bolua (6—B. turkiyae, 7—B. balıkesirensis, 8—B. bursaensis). 9–11. Male cercus in Bolua (9—B. turkiyae, 10—B. balıkesirensis, 11—B. bursaensis). 12–14. Male subgenital plate in Bolua (12—B. turkiyae, 13—B. balıkesirensis, 14—B. bursaensis). 15–17. Titillators in Bolua (15—B. turkiyae, 16—B. balıkesirensis, 17—B. bursaensis)

FIGURES 18–26. 18–20. Female head+pronotum+tegmina in Bolua (18—B. turkiyae, 19—B. balıkesirensis, 20—B. bursaensis; D—dorsal view, L—lateral view). 21–23. Female subgenital plate in Bolua (21—B. turkiyae, 22—B. balıkesirensis, 23—B. bursaensis; V—ventral view, L-lateral view). 24–26. Ovipositor in Bolua (24—B. turkiyae, 25—B. balıkesirensis, 26— B. bursaensis)

FIGURES 28–30. Song in Bolua (28—B. balikesirensis from Balıkesir; 29—and 30—B. bursaensis from Bursa and Kütahya, respectively; A, B and C show song at three different time scale)