Ablabesmyia tucuxi Neubern et Fusari

Fusari, Lívia Maria, Neubern, Caroline S., Hamada, Neusa & Roque, Fabio O., 2012, New species of Ablabesmyia Johannsen from the Neotropical region: first report of a sponge-dwelling Tanypodinae, Zootaxa 3239, pp. 43-50 : 44-49

Ablabesmyia tucuxi Neubern et Fusari sp. n.

( Figs 1–19 )

Type material. Holotype: ♂, BRAZIL, Roraima, Boa Vista municipality, Cauamé River, (slide mounted in Euparal), 02°52’06”N 60°44’24”W, 28–30.v.2009, L. M. Fusari & A. Fernandes ( INPA). Paratypes: 2 ♂, 2 pharate pupae, 2 pharate pupae with larval exuviae ( MZUSP); 2 ♂, 2 pharate pupae, 2 pharate pupae with larval exuviae ( INPA); 5 larvae ( MZUSP), 6 larvae ( INPA), same data as holotype. Bonfim municipality, Igarapé Arraia, 03°21’01”N 59°54’14”W, 22.iii.2001, S. R. M. Couceiro, 6 larvae ( INPA).

Diagnostic characters. Ablabesmyia tucuxi differs from other species by the combination of the following characters. Male - colored legs, tapered megaseta, grooved aedeagal blade and small dorsal lobe; Pupa - thoracic horn with respiratory atrium and with hexagonal pattern; aeropyle tube apex club-shaped; tergite VII without lateral setae. Larva - conic head and exclusive chaetotaxy; ligula with 5 small teeth on each side; A1 and maxillary palp length; palpal formula 2+0; posterior parapods apex without hooklets, 3 hooked claws and 2 small claws situated among the large claws.

Etymology. Informal name given to inhabitants of the Brazilian state of Roraima; the name is to be regarded as a noun in apposition.

Male (n = 5, unless otherwise stated)

Dimensions: Total length [3.60]– 3.74 mm. Wing length [1.50]– 1.66 mm. Total length/wing length 2.25–[2.40]. Wing length/length of profemur [2.11]–2.20.

Coloration ( Figs 1, 4, 5 ): wing with dark spots. Leg: fore leg - femur with 3 bands, tibia with 5 bands; mid leg and hind leg - femur with 3 bands, tibia with 4 bands. Length and proportion of bands on legs as in Table 1. Abdomen like as in Figure 5 .

A/B LMB/LBCB Y/Z

P I 0.65–0.75 1.21–2.25 0,37–0,43 P II 0.64–0.73 1.0–1.27 0.33–0.38 P III 0.60–0.64 0.59–0.82 0.36–0.41 Head ( Fig 2 ): antenna with 13 flagellomeres, AR [1.89] 1.80–2.0, apical flagellomere 94–[106] μm long, subapical seta [36] μm long (n=1). Temporal seta 20–[29] long. Tentorium [185]–208, μm long, stipes not measurable. Clypeus [92]–111 μm long, [110] 95–111 μm wide at widest point, bearing [21]–24 setae. Cibarial pump with anterior margin concave, [254]–280 μm long and with orifice [46]–58 from apex. Labrum [63]–86 μm long, [76] 68–77 μm wide and with 3 setae. Palpomeres1–5 lengths (μm): [45]–52; [90] 88–98; [126] 125–134; [128] 125–134; [200]; palpomere3 with sensilla capitata 34–[37] μm from base.

Thorax ( Fig 3 ): antepronotum with [17]–19 lateral setae; acrostichals and dorsocentrals not discernible; prealars [17]–18; supraalars [1]; scutelars [23]–34.

Wing ( Fig 1 ): width 0.45–[0.46] μm, width/length [0.28]–0.31. C [1.43] 1.42–1.44 mm long, not protruding beyond R4+5. Sc 0.75–0.82 mm long. VR [0.83]–0.85. MCu [437.5] 430–480 μm from arculus. Brachiolum with [3]–4 setae. Squama with 27 setae (n = 1).

Legs ( Fig 4 ): Fore leg - apex of tibia [52]–75 μm wide and with 1 apical pectinated spur [49]–56 μm long, spur ratio 0.50–[0.56]. Mid leg - apex of tibia [54]–69 μm wide and with 2 apical pectinated spurs [61.5]–71 and [40]– 48.5 μm long, spurs ratio [0.45] 0.42–0.51 and 0.42–[0.77]. Hind leg - apex of tibia [60]–70 μm wide and with 2 apical pectinated spurs [63]–70 and [43]–60 μm long, spur ratio 0.49–[0.54] and 0.45–[0.57]. Lengths and proportions of leg segments as in Table 2.

fe ti ta1 ta2 ta3 ta4

P I 712.5–756 869–944 637.5–650 394–450 300–319 206–225 P II 806–906 819–919 625–875 337.5–400 275–300 194–212.5 P III 744–825 941–1156 806–825 437.5–456 362.5–381 187.5–250 continued.

ta5 LR BV SV BR P I 125–150 0.69–0.73 2.0–2.16 2.48–2.62 3.18–6.57 P II 125–137.5 0.76–1.0 2.40–2.66 1.98–2.61 4.45–4.81 P III 125–137.5 0.81–0.88 2.17 2.0–2.16 4.80–5.22 Hypopygium ( Figs 6, 7 ): tergite IX with [3] 2–4 central setae. Anal point covers the aedeagal complex. Sternapodeme [141] 140–146 μm long. Phallapodeme [61.5]–63 μm long. Gonocoxite [154]–180 μm long and [121.5] 118.5–144.5 μm wide. Gonostylus 157–[161.5] μm long, apex with 5 teeth and tapered megaseta. HR [0.95]–1.15. HV [2.23]–2.38. Aedeagal complex: lateral arc absent, grooved aedeagal blade [38.5]–53 μm long, base [9] (n=1) μm wide, 9/10 with [7] μm (n=1) width, dorsal lobe [11]–15 μm long, lateral and basidorsal lobes present.

Female: unknown.

Pupa (n = 3, unless otherwise stated)

Dimension: male abdomen 2.5–3.7 mm long.

Coloration: exuviae yellow; wing sheath without spots ( Fig 13 ).

Cephalothorax ( Figs 8, 9 ): wing sheath 0.70–1.25 mm long and veins absent. Thoracic horn 229–290 μm long and 80–95 μm wide; without membranes; reticulation of respiratory atrium with hexagonal pattern; preapical papilla and plastron plate not discernible, aeropyle tube 37.5 (n=1) μm long and with club-shaped apex; basal lobe present. Thoracic comb with 9–11 tubercles.

Abdomen ( Figs 10, 11, 12 ): tergites II–VII with shagreen composed of small spinules or serially arranged in transverse arched rows. Tergite I with scar 72.5–132.5 μm long. Chaetotaxy on segment IV as in Figure 10 . Tergite VII without taeniae. Tergite VIII with 5 taeniae, LS1 52.5–60 μm. Anal lobe 300–315 μm long and 412–430 μm wide; with 2 anal macrosetae, LS1 72.5–100 μm, LS2 200–230 μm; outer and inner margins spinulate. Genital sac 137.5 μm long. GS/AL 0.44–0.46.

4th instar larva (n = 3, unless otherwise stated)

Coloration ( Figs 14, 17 ): head yellow, without spots; postoccipital margin brown; second antennal segment and maxillary palp pale yellow; apex of mandible brown; ligula teeth yellow; abdomen pale yellow; procercus yellow; posterior parapod with 3 brown claws.

Head ( Figs 14–16, 18, 19 ): conic; 500–560 μm long, 325–400 μm wide; posterior region with crenulations; IC 0.58–0.78. Chaetotaxy as in Figure 15 . Antenomeres1,2-5: 162.5–182.5, 37.5–44 μm long; A1 with ring organ located 63–75 μm from base; AR 3.91–4.38. Maxillary palp with 2 segments; palpomeres1–2: 7.5–12.5; 10–12.5 μm long; palpal formula 2+0; P1/P2 0.66–1.11; A1/P 1 14–23; A2/P1 2.80–4.60. Mandible 72.5–80 μm long, with 1 sensillum campaniformium 20 μm from apex; A1/MD 2.10–2.43. Dorsomental teeth not discernible. Hypopharyngeal complex: ligula 37.5–44 μm long; maximum width 25.5–25 μm, with 5 small teeth on each side and with central space; muscle attachment 12.5 μm long and 30 μm wide; bifid paraligula 14 μm long. Pecten hypopharyngis absent.

Body ( Fig. 17 ): procercus 90–100 μm long and 22.5–37.5 μm wide, with 7 anal setae 230–300 μm long. Anal tubules not measurable. Posterior parapod apex without spinulates; with 12 simple claws with border slightly serrated, with 3 hooked claws and 2 small claws situated among the large claws.

Figures

FIGURES 1 – 7. Ablabesmyia tucuxi sp. n., adult male. 1. Wing. 2. Temporal setae. 3. Antepronotal lobe. 4. Legs: foreleg, midleg, hindleg. 5. Abdomen. 6. Aedeagal complex. 7. Hypopygium: left, dorsal view; right, ventral view. Scales: Figs 1 = 2.0 cm; 2, 3, 7 = 0.1 mm; 5 = 3.2 cm.

FIGURES 8 – 13. Ablabesmyia tucuxi sp. n., pupa. 8. Wing sheath. 9. Thoracic horn. 10. Abdominal segments with chaetotaxy of AIV, anal lobe and male genital sac. 11. anal lobe and male genital sac. 12. Pharate pupae. 13. Pupa and larvae exuviae associated. Scales: Figs 8 – 11 = 0.1 mm.

FIGURES 14 – 19. Ablabesmyia tucuxi sp. n., larva. 14. Head. 15. Head with chaetotaxy, left: dorsal aspect, right: ventral aspect. 16. Ligula and paraligula. 17. Posterior parapod. 18. Antenna apex. 19. Maxillary palp. Scales: Figs 15 = 0.5 mm; 16, 17 = 0.1 mm.