Diamphipnoa fresiae, Sánchez, 2018

Sánchez, Alejandro Vera, 2018, Two new species of Diamphipnoidae (Insecta: Plecoptera) from Chile, with description of adults and eggs, Zootaxa 4527 (1), pp. 49-60 : 50-54

publication ID	https://doi.org/ 10.11646/zootaxa.4527.1.4
publication LSID	lsid:zoobank.org:pub:E0F37BA8-A163-47F8-ADCF-B4C720B2185A
DOI	https://doi.org/10.5281/zenodo.5952046
persistent identifier	https://treatment.plazi.org/id/039087D5-6106-FF9F-FF6C-FD16FE21FBBA
treatment provided by	Plazi
scientific name	Diamphipnoa fresiae
status	sp. nov.

Treatment

Diamphipnoa fresiae View in CoL sp. nov.

( Figs. 1–4 View FIGURE 1 View FIGURE 2 View FIGURE 3 View FIGURE 4 )

Type material. Holotype male: Chile, Región Metropolitana, Cordillera Province, Cajón del Maipo , La Obra , 27 XI 1999, coll. A. Vera ( MNNC) . Paratypes: 5 females, Chile, Región Metropolitana, Cordillera Province, Cajón del Maipo , El Manzano 28 XI 2004 (1), 18 XII 2005 (2), 17 VII 2006 (2), coll. A. Vera ( MNNC) .

Etymology. The patronym honors Dr. Fresia Rojas, National Museum of Natural History of Chile for supporting my initial studies in entomology.

Diagnosis. A small species for the genus. General coloration dark green marked with brown, male with reddish tibiae. Subgenital plate of the male emarginated, tergum X truncate, paraprocts long, epiproct slightly sigmoidal with flexed apices, short and thick, ¾ of the dorsal margin with pairs of teeth. Female subgenital plate slightly sclerotized, broadly emarginated, U-shaped; spermatheca with a long duct, rolled to the right around the capsule within segment IX, oval sperm capsule. Eggs with similar poles, polar disc flat and depressed, equatorial annulus as a fibrous band, follicular cells prominent on the margin of annulus.

Description. Adults habitus: Small for family; general color dark green marked with brown ( Fig. 1F View FIGURE 1 ); forewings with brown spots, apex with oval spots around transversal veins; hindwings translucent, with costal margin brown; oval spots surrounding intercalary costal veins.

Holotype male: Total length 17 mm; antennae length 22 mm; cercus length 11 mm; head width 4 mm; pronotum width 4 mm; pronotum length 2 mm; forewing length 19 mm.

Female (n=5); total length 24–32 mm; antennae length 2 4–27 mm; cercus length 10–11 mm; head width 4–5 mm; pronotum width 4–5 mm; pronotum length 3–4 mm; forewing length 32–36 mm.

Male head ( Fig. 1A View FIGURE 1 ): greenish brown, anterior ocellus small, distance between the lateral ocelli 0.5X greater than with the anterior ocellus; with dark spots around the ocelli, the region anterior to the middle ocellus and a transverse line between the eyes passing over the middle ocellus; clypeus, labrum and antennae brown; antennae with greenish-brown scape and pedicel, flagellum with>73 flagellomeres; first ¼ of the flagellum alternating dark brown and light bands; labium, maxilla and palp gray.

Thorax: Pronotum transverse, width similar to the head; medial apex of anterior margin not prominent; rounded angles, posterior angles broadly convergent towards posterior; general coloration greenish brown as in Fig.1A View FIGURE 1 ; prosternum with truncated anterior margin ( Fig. 1D View FIGURE 1 , female); mesonotum dark brown, with light middle line and metanotum light brown.

Legs: Three pairs of similar coloration, anterior femur greenish-brown with irregular depigmented spots, flexor and extensor margin with interrupted black spots; tibiae reddish, with around 6 to 7 dark bands ( Fig. 1A View FIGURE 1 ); ventral face of femur and tibiae pale-brown; tibiae with two ventral apical spurs; femur with fine setal fringes on the flexor and extensor margin; tarsi brown, first and second tarsomeres with membranous pads developed as ventral extensions; large arolium.

Wings as in Fig. 1B View FIGURE 1 ; light brown and green in the base; with reduced reticulate venation; rounded apex; both pairs with three bifurcations in RP and two in M; hindwing with two bifurcations in αAA2.

Abdomen: Terga brown-greenish; tergum X in dorsal view trapezoidal, wide at base, narrowing to apex, posterior angles rounded, with a posterior-medial V-shape, sclerotized emargination with acute margin ( Fig. 2D View FIGURE 2 ); dorsally flat ( Figs. 2A, B View FIGURE 2 ), the medial emargination continues in a ventral long toothed plate as in the Fig. 2B View FIGURE 2 . Epiproct toothed on basal ½ dorsal margin, with 17 pars of similar teeth directed anteriorly, distal part of epiproct with triangular shape in lateral view ( Fig. 2B View FIGURE 2 ); ventral lobule large and slightly sclerotized. Paraprocts ( Figs. 2E, F, G View FIGURE 2 ) cylindrical, slightly sclerotized; lateroventral sclerite curved up wards, with subapical and middle heavily sclerotized spine as in Fig. 2F, G View FIGURE 2 ; ventro-medial crown of irregular spines ( Figs. 2E, F View FIGURE 2 ); digitiform ventro-basal lobule little sclerotized; apex with membranous lobe mid-dorsal digitiform, short and rounded ( Figs. 2E, F View FIGURE 2 ); this lobe is invaginated at rest. Subgenital plate triangular with emarginated apex ( Fig. 2C View FIGURE 2 ).

Female: Similar to the male but larger in body size and length of the wings; pronotum with narrow posterior angles ( Fig. 1C View FIGURE 1 ); tibiae with 6 to 7 irregular transversal dark brown bands interspersed with greenish gray. Both pairs of wings are acuminate between ScP+RA and M; with more reticular venation and a greater number of bifurcations than male`s wing, ( Fig. 1E View FIGURE 1 ).

Abdomen ( Fig. 3 View FIGURE 3 ): dorsal brown to greenish; tergum X triangular, flat; paraproct subrectangular, apex rounded ( Fig. 3A View FIGURE 3 ). Subgenital plate ( Fig. 3A View FIGURE 3 ) small, with an M-shape, formed by two parallel sclerotized plates pointing at the sides anteriorly, bilobed posterior margin with rounded and slightly prominent edges, middle emargination broadly U-shaped; the middle band and the central region sclerotized; lateral sclerite slightly sclerotized; opaque surface with transverse striae.

Bursa copulatrix as a piriform sac; dorsal sclerites scarcely sclerotized, anterior and posteriorly curved; midline partially sclerotized at the posterior end and around the spermatic pore; anterior sclerite as a scarcely sclerotized plate; lateral sclerites with lanceolate shape, its internal face with smooth transverse wrinkles; both sclerites forming a wide camera in U-shape, hardly convergent towards the previous one; sclerotized ventral plate with six to seven transverse and parallel striae. Spermatheca united in the dorsal half of the bursa; a straight duct extends through segment IX; rolls to the left around the capsule ( Fig. 3C View FIGURE 3 ); capsule oval and longitudinally disposed; the duct is five times as long as the capsule; the capsule and the turns of the duct are located on segment IX.

Eggs: Cylindrical shape ( Fig. 4F View FIGURE 4 ), diameter 0.58– 0.55mm, length 0.38– 0.33mm; with two similar poles, flat polar disc, depressed and limited by a wide edge ( Figs. 4C, E View FIGURE 4 ); without collar; in equatorial view the margins converge towards the poles ( Fig. 4A View FIGURE 4 ), the equatorial annulus with prominent margins, undulating irregularly, distorting the symmetry of the hemispheres, annulus of width similar to the sum of the margins ( Figs. 4A, C View FIGURE 4 ).

Chorion surface with a perimeter band pattern; annulus with a fibrous band; annular margins composed of 2 to 3 rows of compact follicular cells, flat and with tiny air cells ( Figs. 4B, D View FIGURE 4 ); above the margin the follicular cells are weakly developed and progressively more demarcated towards the edge of the polar disc, which has no impressions ( Fig. 4E View FIGURE 4 ).

Numerous micropyles such as elevated perforations located in the peripheral wall ( Fig. 4B View FIGURE 4 ).

Remarks. In one of female paratype the forewings has RP with 4 bifurcations, M 2–3 bifurcations, and CuA with 2–3 bifurcations.