Pantanalinema rosaneae Vaz et al. 2015

Lee, Ok-Min, 2022, Newly recorded genera and species, Pantanalinema rosaneae and Alkalinema pantanalense (Leptolyngbyaceae, Cyanobacteria) isolated in Korea, Journal of Species Research 11 (1), pp. 10-21 : 13

Pantanalinema rosaneae Vaz et al. 2015 ( Fig. 2 )

Morphology and description

The filaments are entangled and flexuous. The cells are slightly constricted, and the cross wall is translucent. The sheath is hyaline, attached to the trichome and always present. Cells are isodiametric or wider than they are long, 1.5-3.2 μm long by 1.8.-2.5 μm wide. The apical cell is cylindrical with a rounded apex. Cell content is homogeneous and brownish green. As above, morphological characteristics were consistent with the previously reported description of this species ( Vaz et al., 2015).

Ecology: This species was isolated in water samples collected from Salina Verde in the Pantanal wetlands, Mato Grosso do Sul State, Brazil ( Vaz et al., 2015). In this study, this species was isolated from the soil of a stone wall, Seoul of Korea.

Site of collection: Chungjeong-ro 3-ga, Seodaemun-gu , Seoul (37°33 ʹ 51.1 ʺ N / 126°57 ʹ 38.6 ʺ E) .

Date of collection: July 24, 2020.

Specimen Locality: FBCC-A1470, FBCC-A 1471 in the Nakdonggang National Institute of Biological Resources (NNIBR).

Gene sequences: The 16S rRNA to 23S rRNA gene sequences: GenBank accession Nos. MZ567050 , MZ56 7052.

16S rRNA gene and phylogenetic affiliation of Pantanalinema rosaneae

The region from 16S rDNA to 23S rDNA were sequenced for two strains ( MZ567050 , MZ567052 ) of Pantanalinema rosaneae . Upon DNA analysis, 16S rDNA were found to be nearly identical to that of the type strain (DNA similarity 99.9%). Phylogenetic relationships of the genus Pantanalinema and other relatives were investigated by using a ML tree constructed from their partial 16S rRNA gene sequences. The additional Bayesian tree turned out to be quite close to the ML tree ( Fig. 4 ). The strains of P. rosaneae (FBCC-A1470, FBCC-A1471) clustered with the type strain P. rosaneae (CENA 516), inferring that the strains of P. rosaneae (FBCC-A1470, FBCC-A1471) and P. rosaneae (CENA 516) were the same species.

Pantanalinema rosaneae FBCC-A1470 showed a 100% DNA similarity to P. rosaneae FBCC-A1471 and 0.00% p -distance between them. On the other hand, the strains of P. rosaneae (FBCC-A1470, FBCC-A1471) showed a 99.5 to 99.9% DNA similarity to P. rosaneae (CENA 516, 517, 521, 537) and 0.00% p -distance between them ( Table 1 ).

Figures

Fig. 2. Microphotographs of Pantanalinema rosaneae from the strain FBCC-A1471. (A-C) Colonies(mats) formed in culture, (D-F) Hormogonia, (G-I) Morphologies of apical cells, (J-L) Firm sheathes of trichomes in culture. Scale bars=(A) 100μm, (B-L) 10μm.

Fig. 4. Phylogenetic position of Pantanalinema and Alkalinema within the family Leptolyngbyaceae inferred from 16S rRNA sequences with the maximum-likelihood (ML) algorithm. 16S rRNA sequences of genera (Gloeobacter violaceus, Accession No. NR074282/ Gloeobacter kilaueenis, Accession No. NR121745) were included as the outgroup. Additionally, the probability of Bayesian analysis was incor- porated into the ML tree to support the strength of each branch. The first and second numbers at the nodes display the bootstrap proportions (BP) (>50%) in ML and posterior probabilities (PP;>0.50) in Bayesian analysis, respectively. The branch lengths are proportional to the scale given.