Chroogomphus conacytiensis Ayala-Vásquez, Pérez-Moreno, Ramírez-Carbajal & González-Martínez, 2023

Pérez-Moreno, Jesús, Martínez-Reyes, Magdalena, Martínezgonzález, César Ramiro, Ramírez-Carbajal, Elisette, Carrera-Martínez, Anaitzi, Fuente, Javier Isaac De La, Olvera-Noriega, Joan Windhoek & Ayala-Vásquez, Olivia, 2023, Two new species of Chroogomphus (Gomphidiaceae, Boletales) with biocultural importance in the Tlahuica-Pjiekakjoo culture from Central Mexico, Phytotaxa 579 (4), pp. 289-298 : 293-294

Chroogomphus conacytiensis Ayala-Vásquez, Pérez-Moreno, Ramírez-Carbajal & González-Martínez , sp. nov. ( Fig. 2 )

Mycobank no. 845567, GenBank: OP580489 (ITS).

Diagnosis: —Basidiomata small to medium, pileus surface furfuraceous to velvety, brown, brown-yellowish, brownolivaceus, grey to greyish, sterile margin, when touch reddish-brown to vinaceous. Basidiospores 13–16(–19) × 6–7 (–8) μm, Q=2.2, (n=34), ellipsoid, golden yellow to yellowish-yellow on KOH.

Type: — MEXICO, STATE OF MEXICO: Ocuilan Municipaly, Lomas de Teocaltzingo Town , mixed QuercusPinus forests, 462878N; 2102619W, 2989 m, 9 September 2021, Martínez-Reyes M., Ayala-Vásquez O, Pérez-Moreno J. ( MEXU-HO 30431, holotype) .

Description: — Pileus 23–35 mm diameter, broadly convex somewhat umbonate when young, hemispherical towards at maturity, pale yellowish-brown (5D8–5E8), brownish-orange (5C3–5D3), grey (30D1–30E1), greyishbrown (5D3), brownish-grey (6D2–6F2) to dark brown (6F8), when touch reddish-brown (8D6–8E6) to vinaceous, humid surface, viscid when wet, smooth, shining when dry, margin enrolled when young, margin with curtain remnants at mature. Hymenophore Lamellae, subdecurrent, distant, orange-greyish (5B4–5B5) to light-brown (6D4–6E4), when touch reddish-brown (8D6–8E6) to vinaceous, lamellae 7–10 mm width, slightly serrate, irregular lamellulae common, attenuate. Context pileus 8–10mm thick, light orange (5A4), pale orange (5A5) to salmon, basal context of the stipe orange (5A6), immutable to court. Stipe 23– 70 × 6– 14 mm, cylindrical, orange-yellow (4A8), orange (5A4), paleorange (5A3–5A4), when cut it becomes greyish-vinaceous (12E7) to deep vinaceous (12F8), sometimes slightly attenuate downwards, fibrillose surface with ring ephemeral remains very close to the apex whiting to pale pink (12A4). Chemical reaction when applying KOH, all basidiomata turn vinaceous.

Basidiospores (13–)17–18– 20(–23) × 5–6 (–7) µm, (L = 17.89, W = 6.1, Q = 2.5 –2.7 (–2.9), N = 65), cylindrical to elliptical, fusiform, smooth, brown-yellowish, thick-walled. Basidia (35–) 37–55–64 (–70) × (7–) 10–14 (–15) µm, clavate, hyaline to pale brown in KOH, brown-chocolate, in Melzer’s reagent, with long sterigmata (5–) 7–8× 2–3 µm. Cystidia (100–) 120–150 (–150) × (15–) (12–)17–21(–22) µm, fusoid-capitate, subclavate to subfusiform, thickwalled (≤3), hyaline to brown in KOH, brown-yellowish in Melzer’s reagent. Lamellar trama made up of hyphae 4–6 µm in width, cylindrical, hyaline to brownish in KOH. Pileipellis formed by ixotrichoderm, with terminal cells (14–) 25–45–60 (–70) × (8–) 10–15–20 (–30) µm, clavate, fusiform, to ventricose-fusoid, brown- vinaceous, to chocolate pigment but soon dissolved in KOH, with gelatinous wall that disappears with Melzer’s reagent, amyloid. Stipitipellis composed of light amyloid, vertical hyphae (4–)5–12 µm in diameter, thick walled (≤2). With clamp connections.

Etymology: —The name is in honor of the Mexican Council of Science and Technology (or CONACYT for its abbreviation in Spanish) which is the public institution responsible for promoting the advancement of scientific research, innovation and technological development, including that of food security and mycocultural heritage, of the country during the last half-century.

Habitat, habit, and distribution: —Solitary to scattered growing under mixed forest to Quercus-Pinus forests, forming ectomycorrhizal associations with Pinus pseudostrobus and P. hartwegii , during July to September at altitudes between 2900 to 3000 m. Only known currently from Central and Southwestern Mexico.

Specimens examined: — MEXICO, STATE OF MEXICO: Ocuilan Municipaly, Lomas de Teocaltzingo Town, mixed forest, 4664720 N; 2111033 W, 3390 masl, 9 September 2021, Mycoredes (MEXU-HO 30426 paratype); OAXACA: Santa María Tlahuitoltepec Municipaly, Bridge to Zempoaltepetl Town, Pinus hartwegii forest, 462878 N; 2102619 W, 3450 masl, 15 July 2022, Ayala-Vásquez O, Martínez-Gonzalez CR., Martínez-Reyes M., (MEXU-HO-30452); STATE OF TLAXCALA: Nanacamilpa Municipaly, San Felipe Hidalgo Town, El Astillero, 2900 masl, 15 September 2021, Martínez-Reyes M., Ayala-Vásquez O, (MEXU 30451).

Figures

FIGURE 2. Chroogomphus conacytiensis (MEXU-HO 30431, holotype, MEXU-HO- 30452 paratype). A. General view of basidiomata. B. Basidioma. C. Stipe surface and partial velum. D. Basidiospores. E. Basidium and cystidium. F. Basidia. G. Lamellar trama. H–I. Two different cystidia forms. J–L Pileipellis. Scale bars: 10 mm (A–C); 10µm (D, E, F, H, I, K, L); 100 µm (G,); 25 µm (J).