Phaeodactylum tricornutum, Bohlin, 1897

Nikitashina, Vera, Stettin, Daniel & Pohnert, Georg, 2022, Metabolic adaptation of diatoms to hypersalinity, Phytochemistry (113267) 201, pp. 1-13 : 4

2.7. Comparison of salinity stress responses of P. tricornutum , T.

pseudonana, and S. marinoi

We compared the amount of unique and shared metabolites regulated in response to salinity stress between the three diatoms. Surprisingly, only ca. 6% after 24 h and 4% after 96 h of the metabolites were common for a salinity adaptation in all three investigated species. This corresponds to 22 common metabolites (8 detected in GC-MS, 13 in LC-MS, and proline detected with both methods) that were significantly dysregulated in all species at least at one time point. All of them were up-regulated.

A pairwise analysis shows that P. tricornutum and T. pseudonana share more commonly dysregulated metabolites compared to S. marinoi and T. pseudonana or S. marinoi and P. tricornutum ( Figs. 4 and 5 ). The largest share of dysregulated metabolites was unique for individual algal species and the vast majority of all dysregulated metabolites were up-regulated upon salinity increase.

Figures

Fig. 4. Venn diagrams for significantly dysregulated compounds of all identification levels for P. tricornutum (blue), T. pseudonana (green), and S. marinoi (yellow) detected with GC-MS at 24 h (A) and 96 h (B) after the salinity stress. The percentage of the total number of compounds detected for all three algae is given and numbers in brackets indicate the amount of compounds. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Fig. 5. Venn diagrams for significantly dysregulated compounds of all identification levels for P. tricornutum (blue), T. pseudonana (green), and S. marinoi (yellow) detected with LC-MS at 24 h (A) and 96 h (B) after the salinity stress. The percentage of the total number of compounds detected for all three algae is given and numbers in brackets indicate the amount of compounds. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)