Cornutheca ansata Leidy, 1879 Kosakyan, Lahr, Mulot, Meisterfeld, Mitchell and Lara

Anush Kosakyan, Daniel J. G. Lahr, Matthieu Mulot, Ralf Meisterfeld, Edward A. D. Mitchell & Enrique Lara, 2016, Phylogenetic reconstruction based on COI reshuffles the taxonomy of hyalosphenid shelled (testate) amoebae and reveals the convoluted evolution of shell plate shapes, Cladistics 32, pp. 606-623 : 622

Cornutheca ansata Leidy, 1879 Kosakyan, Lahr, Mulot, Meisterfeld, Mitchell and Lara

Description. Test pyriform in broad lateral view, laterally compressed, with a pair of hollow horns protruding, one on either side of the neck one-third of the distance from the pseudostome to the fundus. Test almost transparent or slightly yellowish – brownish, composed of small oval particles probably predated from small euglyphids. Dimensions: L = (195) 220 – 264 (270) µm, B (between the ends of the lateral horns) = (110) 130 – 150 (169) µm, B (of the main body) = 90 – 125 µm, horn length = 25 – 59 µm. Pseudostome = 40 – 55 µm, slightly curved in broad lateral view.

Type. Plate 25, Figs 1 – 8 ( Leidy, 1879).

Figures

Fig. 1. Schematic demonstration of measured axes of the test: (1) length, (2) breadth, (3) width of aperture (pseudostome) and (4) width of the shell plates (scales).

Fig. 2. Light micrographs of barcoded Quadrulella variabilis sp. nov. cells: (a) cell Q- 54; (b) cell Q- 48; (c) cell Q- 52; (d) cell Q- 101; (e) cell Q- 98; (f) cell Q- 53. Scale bars = 10 µm.

Fig. 3. Light micrographs of barcoded Quadrulella symmetrica s. s. cells: (a) cell Q- 75; (b) cell Q- 83; (c) cell Q- 81; (d) cell Q- 90; (e) cell Q- 63; (f) cell Q- 73; (g) cell Q- 51; (h) cell Q- 102; (i) cell Q- 100; (j) cell Q- 95; (k) cell Q- 82; (l) cell Q- 51. Scale bars = 10 µm.

Fig. 4. Light micrographs of two Quadrulella madibai sp. nov. barcoded cells: (a) cell Q- 11; (b) cell Q- 9. Scale bars = 10 µm.

Fig. 5. Light (a) and scanning electron (b) micrographs of Quadrulella quadrigera from Australia. Image (a) has been stacked with Combine ZP. Scale bars = 40 µm.

Fig. 6. Light micrographs of barcoded Quadrulella alata cells: (a) cell Q- 8; (b) cell Q- 7; (c) cell Q- 17; (d) cell Q- 15. Scale bars = 20 µm (in a and b) and 50 µm (in c and d).

Fig. 7. Light micrographs of three barcoded Mrabella subcarinata cells (previously known as Quadrulella subcarinata): (a) cell Q- 1; (b) cell Q- 2; (c) cell Q- 16. Scale bars = 20 µm (in a and b) and 50 µm (in c).

Fig. 8. Scanning electron micrographs illustrating morphological variations within the Quadrulella cf. symmetrica morphospecies. (a) Q. cf. symmetrica _ BG from Bulgaria; (b) Q. cf. symmetrica _ CA from Canada; (c) Q. cf. symmetrica _ CH from Switzerland; (d) Q. variabilis with a long neck (ex: Q. longicollis) from Bulgaria. Scale bars = 20 µm. Image from Kosakyan et al. (2012) with permission from the editor.