Brooksia lacromae, Garić & Batistić, 2016

Garić, Rade & Batistić, Mirna, 2016, Description of Brooksia lacromae sp. nov. (Tunicata, Thaliacea) from the Adriatic Sea, European Journal of Taxonomy 196, pp. 1-13 : 5-10

publication ID

https://doi.org/ 10.5852/ejt.2016.196

publication LSID

lsid:zoobank.org:pub:38CC4797-768A-44C5-8B85-EA3DCB6678CB

DOI

https://doi.org/10.5281/zenodo.3852158

persistent identifier

https://treatment.plazi.org/id/2B9ACCBD-8B20-437D-B013-468F8E956864

taxon LSID

lsid:zoobank.org:act:2B9ACCBD-8B20-437D-B013-468F8E956864

treatment provided by

Valdenar

scientific name

Brooksia lacromae
status

sp. nov.

Brooksia lacromae View in CoL sp. nov.

urn:lsid:zoobank.org:act:2B9ACCBD-8B20-437D-B013-468F8E956864

Figs 1–4 View Fig View Fig View Fig View Fig

Diagnosis

Oozooid (solitary form; Fig. 2 View Fig )

Muscles II and III fuse dorsally before fusing with muscle I mid-dorsally. Muscles III and IV fuse briefly dorsolaterally. Muscles IV and V fuse before approaching muscles VI and VII mid-dorsally. There is one longitudinal ventral muscle which extends anteriorly into the proboscis. In addition, the proboscis contains two lateral longitudinal muscles. The ventral longitudinal muscle branches posteriorly into two branches. There are two slit-like openings in the ventral longitudinal muscle, one below the anterior end of the endostyle, and the other slightly anteriorly from the first one. Muscle I fuses with branches of the ventral longitudinal muscle at their anterior part, while muscles VI and VII fuse with them posteriorly. Muscles II, III, IV and V converge ventrally towards the junction between muscle I and the branches of the ventral longitudinal muscle. Muscle II can be slightly fused with muscle I just before its connection with branches of the ventral longitudinal muscle. Endostyle straight. Stolon emerges from the test ventrally, slightly posteriorly from the nucleus. There is one test processus just posteriorly from the stolon ( Fig. 4D View Fig ). Muscles I–VII with 52–58 muscle fibers in total, measured laterally in two examined individuals.

Blastozooid (aggregate form; Figs 3 View Fig , 4 View Fig A–C)

Sinistral individual – Four muscles on the left side, three muscles on the right side. Dorsal IM1 continues ventrally as the ventral muscle IM1, without extending to the anterior attachment processus (ap1). A branch of dorsal muscle IM1 (IM1-a) extends to the anterior attachment processus (ap1). Dorsal muscles IR, IIR and IIIR branch dichotomously. Dorsal muscle IR-a continues ventrally as ventral muscle IR. Dorsal muscle IR-b continues ventrally as muscle IIR-c. A small branch from muscle IR-b/IIR-c enters the lateral attachment processus (ap2). Dorsal muscle IIR-a continues ventrally as ventral muscle IIR-a. Muscle IIR-b ends blindly on the ventral side of the animal without entering the posterior attachment processus (ap3). Dorsal and ventral muscles IIIR enter the posterior attachment processus (ap3) without joining. Muscles IVL-a and IIIR-a extend from muscles IVL and IIIR, respectively, towards the nucleus. Endostyle curved anteriorly. Muscles IL-IIIL, IR-a,b and IIR-a,b,c with 3 muscle fibers each in all examined individuals. Muscle IVL with from 3 to 9 muscle fibers, muscle IIIR with from 5 to 7 muscle fibers.

Dextral individual

Mirror image of sinistral individual.

Etymology

Brooksia lacromae sp. nov. is named after the island of Lokrum near which it has been found. The Latin name of the island of Lokrum is Lacroma.

Material examined

Two oozooids and 19 blastozooids.

Holotype

One oozooid (14.8 mm body length, 23.9 mm total length including proboscis), collected from a 0–50 m depth layer on 3 Oct. 2014 in a 53 μm mesh plankton net. It is deposited in the Tunicata collection of the Croatian Natural History Museum under inventory number CNHM Inv. br. 44/1.

Allotype

One sinistral blastozooid (4.2 mm body length, without attachment processes), collected from a 0–50 m depth layer on 3 Oct. 2014 in a 53 μm mesh plankton net. It is deposited in the Tunicata collection of the Croatian Natural History Museum under inventory number CNHM Inv. br. 44/2

Paratypes

One oozooid (11.5 mm body length, 18.2 mm total length including proboscis) and one dextral blastozooid (4.8 mm body length, without attachment processes), collected from a 50–100 m depth layer on 3 Oct. 2014 using a 53 μm mesh plankton net, deposited in the Institute for Coastal and Marine Research (University of Dubrovnik) under inventory number IMP-002; one dextral blastozooid (4.1 mm body length, without attachment processes) collected from a 0–50 m depth layer on 3 Oct. 2014 with a 200 μm mesh plankton net, deposited in the Dubrovnik Natural History Museum under inventory number PMD 2106.

Other material

16 blastozooids deposited in the Institute for Coastal and Marine research (University of Dubrovnik) under inventory number IMP-003.

Type locality

42°37'21"N, 018°06'05"E, off Dubrovnik, South Adriatic (Mediterranean Sea; Fig. 1 View Fig ).

The temperature average in the 0–50 m layer was 19.0°C, with a range between 16.2°C and 22.2°C, while the salinity average was 38.36, with a range between 37.79 and 38.68. In the 50–90 m layer the temperature average was 15.8°C, with a range between 15.5°C and 16.1°C, while the salinity average was 38.71, with a range between 38.67 and 38.77.

Remarks

Oozooid. Brooksia rostrata and Brooksia berneri oozooids are very similar, differing only in the fact that in B. berneri oozooid muscle I, joined with the intermediate muscle (im), is separated from muscle II and discontinuous mid-dorsally. Due to this fact we will only compare the B. lacromae sp. nov. oozooid to the B. rostrata oozooid. The Brooksia lacromae sp. nov. oozooid has one ventral longitudinal muscle which extends into the proboscis, while B. rostrata has two ( Fig. 2 View Fig ). The proboscides in both collected oozooids broke off during collection, but were found in the sample ( Fig. 2c View Fig ). The proboscis of Brooksia lacromae sp. nov. seems to be thinner at the base than in Brooksia rostrata and in both species it contains two lateral longitudinal muscles. The ventral longitudinal muscle in B. lacromae sp. nov. has two small slit-like openings anteriorly, one below the endostyle and the other just anteriorly to it, partly overlapping with the anterior end of the endostyle. In Brooksia lacromae sp. nov., in contrast to B. rostrata , body muscles are not arranged in a barrel-like structure where muscles are perpendicular to the body axis dorsally, as well as ventrally, but they converge to the posterior third of the body. Only muscles I, VI and VII fuse with the branches of the ventral longitudinal muscle, while muscles II, III, IV and V converge to the connection between muscle I and branches of the ventral longitudinal muscle, without fusing with either. Only muscle II can sometimes be slightly connected with muscle I ventrally. The stolon in Brooksia lacromae sp. nov. seems to emerge from the test, slightly posteriorly from the nucleus ( Fig. 4D View Fig ), while in B. rostrata it emerges from the anterior part of the nucleus ( Thompson 1948).

Blastozooid (sinistral individual). The Brooksia lacromae sp. nov. and Brooksia rostrata blastozooids are very similar. In Brooksia lacromae sp. nov., muscle IIR-b ends blindly on the ventral side of the animal without entering the posterior attachment processus (ap3), while in Brooksia rostrata it enters the posterior attachment processus (ap3). The left intermediate muscle (IM1) in Brooksia lacromae sp. nov. is continuous dorsally and ventrally. Its branch IM1-a enters the anterior attachment processus (ap1). In Brooksia rostrata the left intermediate muscle is discontinuous. Its dorsal and ventral counterparts both enter the anterior attachment processus. Because of this, the anterior attachment processus in B. lacromae sp. nov. possesses two muscles (IM1-a and IM2), while in B. rostrata it possesses three muscles (dorsal and ventral left intermediate muscle and right intermediate muscle). According to Thompson (1948), the right intermediate muscle of the sinistral individual of B. rostrata ends blindly, while after Tokioka (1954) and Godeaux (1998) it connects to muscle I. In B. lacromae sp. nov. the right intermediate muscle (IM2) ends blindly.

Genetic analysis

There were no differences between blastozooid and oozooid 18S sequences. Between two cox1 sequences there were 7 substitutions out of 837 nucleotides, which results in 0.84% uncorrected pairwise distance. Cox1 sequences were translated using ascidian mitochondrial code and there were no differences in amino acid sequence between them. The uncorrected pairwise distance between the Brooksia rostrata 18S sequence ( HQ015403 View Materials ) and the Brooksia lacromae sp. nov. 18S sequence ( KR057223 View Materials ) was 1.5% (including gaps). Out of 26 differences in 1740 nucleotides between these two sequences, there were 21 transitions, 4 transversions and one single nucleotide gap.

CNHM

Cincinnati Museum of Natural History

Kingdom

Animalia

Phylum

Chordata

Class

Thaliacea

Order

Salpida

Family

Salpidae

Genus

Brooksia

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