Mangrovivirga cuniculi, Sefrji & Michoud & Marasco & Merlino & Daffonchio, 2021

Sefrji, Fatmah O., Michoud, Grégoire, Marasco, Ramona, Merlino, Giuseppe & Daffonchio, Daniele, 2021, MaNgROVIVIRga CUNICULI gen. nov., sp. nov., a moderately halophilic bacterium isolated from bioturbated Red Sea mangrove sediment, and proposal of the novel family MaNgROVIVIRgaCeae fam. nov., International Journal of Systematic and Evolutionary Microbiology (004866) 71 (7), pp. 1-11 : 9

DESCRIPTION OF MANGROVIVIRGA CUNICULI SP. NOV.

Mangrovivirga cuniculi (cu.ni.cu′ li. L. gen. n. cuniculi of a burrow, named because the type species was isolated from a crab burrow).

The cell morphology and chemotaxonomic characteristics are given in the genus description. Cells are 0.3–0.5 µm wide and 1–1.2µm long. Its colonies are circular with a diameter of 1–2mm and feature regular edges, a smooth and shiny surface, and an orange colour caused by the production of orange carotenoids. The permissive conditions for growth are a temperature of 20–40 °C (optimum, 37°C), pH 6–10 (optimum, pH 8) and 3%–11% NaCl for salinity (optimum, 7%–9% NaCl). The carbon sources used for growth are pectin, 2-deoxy-D-ribose,Dribose, 5-keto-D-gluconic acid,L-ornithine, dihydroxyacetone, thymidine, uridine and adenosine. The genome harbours genes responsible for protection against oxidative, osmotic and salinity stresses, and includes genes encoding proteins that produce osmoprotectants and carotenoids. The cells are negative for amylase, protease, lipase, cellulase, indole, siderophore production and phosphate solubilization, and are positive for auxin (IAA) and ammonia production. The strain is unable to reduce nitrate to nitrite.

The type strain, R1DC9 T (=KCTC 72349 T =JCM 33609 T =NCCB 100698 T), was isolated from bioturbated mangrove sediment at the Ibn Sina Field Research Station and Nature Conservation Area in KAUST, Saudi Arabia. The genomic DNA G+C content and genome size of the type strain are 63.1 mol% and 4661901 bp, respectively.